The phenolic composition, antioxidant activity, and antimicrobial activity of extracts from vine leaves six grape varieties collected in May, August, September was studied. potential the dependent on variety picking-time. Extracts were richest total phenols, flavonoids, flavonols, stilbenes. properties determined by ferric reducing power 2,2-diphenyl-1-picrylhydrazyl assays, against Stapylococcus aureus, Bacillus cereus, Campylobacter jejuni, Escherichia coli, Salmonella Infantis good...

The aim of the study was to investigate mode action (-)-α-pinene in terms its modulation antibiotic resistance Campylobacter jejuni. Broth microdilution and ethidium bromide accumulation assays were used evaluate antimicrobial activity, resistance, inhibition efflux. target efflux systems identified using an insertion mutagenesis approach, C. jejuni adaptation evaluated DNA microarrays. Knock-out mutants key up-regulated transcriptional regulators hspR hrcA constructed their roles several...

To define anti-Campylobacter jejuni activity of an extract from waste skins and seeds Pinot noir grapes (GSS), resveratrol possible resistance mechanisms, the influence these on Camp. morphology.Using gene-specific knock-out mutants efflux pump inhibitor, we showed CmeABC as most active for extrusion across outer membrane GSS resveratrol. Using polystyrene surface pig small intestine epithelial (PSI) human foetal (H4) cell lines, shows efficient inhibition adhesion to abiotic biotic...

Abstract Biofilms provide a protective environment for pathogens such as Campylobacter jejuni , the most prevalent foodborne pathogen, and biofilm formation can enhance bacterial survival in hostile environments. Adhesion of bacteria to different materials industrial surfaces is first step formation. Modulation adhesion thus represent important targets alternative control strategies reduction food-processing With high prevalence C. lack effective measures, new are needed block on food...

Campylobacter is a major foodborne pathogen and alternative antimicrobials are needed to prevent or decrease contamination in foods food producing animals. The objectives of this study define the anti-Campylobacter activities natural phenolic compounds plant origin determine roles bacterial drug efflux systems resistance these phenolics jejuni.Anti-Campylobacter were evaluated by an MIC assay using microdilution coupled with ATP measurement. Mutants cmeB cmeF genes cmeR transcriptional...

Thermophilic campylobacters are a major cause of bacterial food-borne diarrhoeal disease. Adherence and biofilm formation key elements Campylobacter jejuni persistence in unfavourable environmental conditions. The phytochemical analysis Euodia ruticarpa fruit ethanol solution extract (EREE) indicated that the compounds were evodiamine (1), rutaecarpine (2) evocarpine (9). E. extract, 1 2 as well mixture quinolinone alkaloids with 41.7% 9 tested for antibacterial, antibiofilm antiquorum...

Pseudomonas are part of the indigenous microbiota different foods, where they gradually cause spoilage. In fish meat, fragi and psychrophila have been identified as important spoilers. The initial aim this study was to investigate physiological characteristics, adhesion, biofilm P. under temperatures related fish-processing industry. further define problem increased growth pathogenic bacteria in presence spoilage vitro meat.Temperature dependence on observed. Hydrophobicity autoaggregation...

Biofilms are the predominant bacterial lifestyle and can protect microorganisms from environmental stresses. Multispecies biofilms affect survival of enteric pathogens that contaminate food products, thus, investigating underlying mechanisms multispecies is essential for safety human health. In this study, we investigated ability natural isolate Bacillus subtilis PS-216 to restrain Campylobacter jejuni biofilm formation adhesion abiotic surfaces as well disrupt preestablished C. biofilms....

Salmonella spp. are a commonly identified cause of outbreaks food-borne diseases. Despite much research, there remains the need to find new antimicrobial and anti-biofilm agents against Salmonella. For this, it is necessary distinguish between these two aspects. Agents that influence biofilm formation should not affect bacterial growth, thus avoid further promotion development resistance. In this study, we present use growth curves Infantis simultaneously determine activities, for screening...

Abstract Interactions between bacterial cells and contact materials play an important role in food safety technology. As strains become ever more resistant to antibiotics, the aim of this study was analyse adhesion selected foodborne on polystyrene surface evaluate effects natural antimicrobials cell hydrophobicity, adhesion, zeta potential as strategies prevention. The results showed strain-specific rate polystyrene. lowest highest were found for two B. cereus lines. Natural ferulic...

In order to survive in food-processing environments and cause disease, Campylobacter jejuni requires specific survival mechanisms, such as biofilms, which contribute its transmission through the food chain human host present a critical form of resistance wide variety antimicrobials.Phytochemical analysis thyme ethanolic extract (TE), post-hydrodistillation residue (TE-R), olive leaf (OE) using high-performance liquid chromatography with photodiode array indicates that major compounds TE TE-R...

Attachment of pathogenic bacteria to food contact surfaces and the subsequent biofilm formation represent a serious threat for industry, since these are more resistant antimicrobials or possess virulence factors. The main aim this study was investigate correlation between antibiotic resistance against 13 antibiotics, distribution 10 factors in 105 Escherichia coli strains according their origin. high prevalence that we have found wildlife isolates could be acquired by horizontal transfer...

During winemaking, grape polyphenols are only partly extracted, and consequently unexploited. The main aim was to characterize the phenolic content of freeze-dried skin seed (FDSS) extracts obtained from Slovenian international varieties evaluate their antioxidant, antimicrobial anti-adhesive activities.FDSS six Vitis vinifera L. grapevine cultivars Vipava Valley region (Slovenia) underwent extraction sonification under different conditions. Flavonols were predominant white 'Zelen'...

Campylobacter jejuni, a widespread pathogen found in birds and mammals, poses significant risk for zoonosis worldwide despite its susceptibility to environmental food-processing stressors. One of main survival mechanisms is the formation biofilms that can withstand various stressors, which why efficient methods assessing are crucial. Existing methods, including classical culture-based plate counting method, biomass-staining (e.g., crystal violet safranin), DNA-staining those use metabolic...

Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination meat products during processing. For infection, must adhere to epithelial cells the mucus layer, survive conditions gastrointestinal tract, and colonise intestine host. Addition probiotic bacteria might promote competitive adhesion cells, consequently reducing jejuni colonisation. Effect Lactobacillus (PCS20, PCS22, PCS25, LGG, PCK9) on C. adhesion, invasion translocation...

The food-borne pathogen Campylobacter jejuni produces autoinducer-2 (AI-2) as an interspecies signalling molecule. AI-2 can trigger enhanced colonisation and biofilm formation, this poses a serious risk to public health. To date, communication system of C. is only partially understood, detection quantification such autoinducer molecules in complex media hard achieve. We have developed whole-cell Vibrioharveyi-based biosensor assay accurately quantify follow production by 81-176 defined...

Campylobacter jejuni is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source infection. To reduce risk associated with C. transmission via meat, effective interventions during production are needed, use probiotics promising approach. In this study, 15 Bacillus subtilis strains were initially screened for their anti-Campylobacter activities. B. PS-216 strain demonstrated best activity against isolates when examined...

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