A5044562747- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Polyomavirus and related diseases
- Bacteriophages and microbial interactions
- Autism Spectrum Disorder Research
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Renal and related cancers
- Animal Virus Infections Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Congenital heart defects research
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
Erciyes University
2021-2025
Université Côte d'Azur
2015-2024
Inserm
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Institut de Recherche sur le Cancer et le Vieillissement de Nice
2021-2024
Centre Azuréen de Cancérologie
2022
Cardiff University
2022
Shahid Beheshti University
2021
Institut de Biologie Valrose
2011-2019
La Ligue Contre le Cancer
2007-2009
Peroxisome proliferator-activated receptors (PPARs) are nuclear exerting several functions in development and metabolism. The physiological of PPARdelta remain elusive. By using a CRE-Lox recombination approach, we generated an animal model for muscle-specific overexpression to investigate the role this tissue. Muscle-specific results profound change fiber composition due hyperplasia and/or shift more oxidative and, as consequence, leads increase both enzymatic activities genes implicated...
Abstract The paternal heredity of obesity and diabetes induced by a high-fat and/or high-sugar diet (Western-like diet) has been demonstrated through epidemiological analysis human cohorts experimental analysis, but the nature hereditary vector inducing this newly acquired phenotype is not yet well defined. Here, we show that microinjection either testis or sperm RNA male mice fed Western-like into naive one-cell embryos leads to establishment diet-induced metabolic in resulting progenies,...
In mice, susceptibility to Leishmania major is associated with the early expansion of T helper 2 cells (T H 2) cells, but nothing known specificity these cells. A previously identified antigen, homolog receptors for activated C kinase (LACK), was found be focus this initial response. Mice made tolerant LACK by transgenic expression antigen in thymus exhibited both a diminished response and healing phenotype. Thus, that are reactive single play pivotal role directing immune entire parasite.
Transfer into mouse and rat embryo fibroblasts in primary culture of cloned polyoma virus genes encoding only the large T protein led to establishment flat colonies sparse subcultures at a frequency equal that transformation by wild-type virus. Cell lines could be derived from such maintained for numbers generations without entering crisis. They exhibited normal phenotype, criteria growth on plastic low saturation density anchorage dependency. However, they required lower serum concentration...
The size of the mammalian body is determined by genetic and environmental factors differentially modulating pre- postnatal growth. We now report a control growth acting in mouse from first cleavages to stages. It was evidenced hereditary epigenetic modification(paramutation) created injection miR-124 microRNA into fertilized eggs. From blastocyst adult, pups born after microinjection this miRNA showed 30% increase size. At stage, frequent duplication inner cell mass resulted twin...
RNA–mediated transmission of phenotypes is an important way to explain non-Mendelian heredity. We have previously shown that small non-coding RNAs can induce hereditary epigenetic variations in mice and act as the transgenerational signalling molecules. Two prominent examples for these paramutations include modulation Kit gene, resulting altered fur coloration, Sox9 overgrowth phenotype. now report expression Dnmt2 RNA methyltransferase required establishment maintenance both paramutations....
The recent discovery of a significant amount RNA in spermatozoa contradicted the previously held belief that paternal contribution was limited to one copy genome. Furthermore, detection sperm raised intriguing question its possible role embryonic development. possibility RNAs may serve as epigenetic determinants supported by experiments showing inheritance traits mice mediated RNA. We used high-throughput, large-scale sequencing technology analyze sequences generated were diverse terms...
ABSTRACT The Kit receptor and its ligand KL, which together constitute an essential effector at various stages of embryonic development, are both present during adult gametogenesis. In the testis, KL is expressed in Sertoli cells, germ starting premeiotic stages. A series observations indicated previously a role spermatogonia survival, without excluding possible function later We identified complex pattern expression two components murine suggestive meiotic progression spermatocytes. At...
Two distinct forms of the myc oncogene were assayed for their ability to induce, in cultured rat fibroblast cells, alterations cellular growth controls observed upon transfer gene polyoma virus encoding only large T protein (plt). Both these rearranged genes and plt had been previously shown cooperate with ras oncogenes transformation embryo fibroblasts (REF) thought induce same early step ("immortalization") tumoral pathway. We now report that two different elicite response following...
Autism spectrum disorder (ASD) is a group of developmental pathologies that impair social communication and cause repetitive behaviors. The suggested roles noncoding RNAs in pathology led us to perform comparative analysis the microRNAs expressed serum human ASD patients. cohort 45 children with revealed six (miR-19a-3p, miR-361-5p, miR-3613-3p, miR-150-5p, miR-126-3p, miR-499a-5p) were at low very levels compared those healthy controls. A similar but less pronounced decrease was registered...
Site-specific methylation of cytosines is a key epigenetic mark vertebrate DNA. While majority the methylated residues are in symmetrical (meC)pG:Gp(meC) configuration, smaller, but significant fraction found CpA, CpT and CpC asymmetric (non-CpG) dinucleotides. CpG reproducibly maintained by activity DNA methyltransferase 1 (Dnmt1) on newly replicated hemimethylated substrates (meC)pG:GpC. On other hand, establishment hereditary maintenance non-CpG patterns have not been analyzed detail. We...
Abstract Purpose Traumatic Brain Injury (TBI) poses a significant global health burden, with Mild TBI (mTBI) being the most prevalent form. triggers activation of hypothalamic–pituitary–adrenal (HPA) axis, which in turn affects hypothalamic-pituitary–gonadal (HPG) axis regulating oogenesis and spermatogenesis. In this study, we investigated impact mTBI on sperm genome integrity using repetitive (r-mTBI) mouse model. Methods We assessed telomere length (TL), free TERRA (fTERRA), DNA/RNA...
Telomeric repeat-containing RNAs (TERRAs) are expressed from subtelomeric regions, as long non-coding responsible for maintaining telomere length and stabilizing the genome. After TERRA is transcribed (f-TERRA) subtelomere it hybridizes (h-TERRA) into telomeres intergenic regions to maintain genome stability. Here, we separately determine changes in two fractions, f-TERRA h-TERRA levels relation (TL) first time meningioma patients with preoperative postoperative comparisons control group....