A5091621849- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Virus-based gene therapy research
- Enzyme function and inhibition
- Hematopoietic Stem Cell Transplantation
- Glycosylation and Glycoproteins Research
- Cancer Cells and Metastasis
- Synthesis and Catalytic Reactions
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Herpesvirus Infections and Treatments
- Biotin and Related Studies
- HIV Research and Treatment
- Pharmacological Effects and Toxicity Studies
- Hedgehog Signaling Pathway Studies
- Chemical Reactions and Isotopes
- Cancer-related gene regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer Research and Treatments
- Polyamine Metabolism and Applications
- Erythrocyte Function and Pathophysiology
- Amino Acid Enzymes and Metabolism
- Blood disorders and treatments
Helmholtz Zentrum München
2009-2019
Ludwig-Maximilians-Universität München
2013
University of Oxford
2013
Institute for Environment and Human Security
1997-2011
Center for Environmental Health
2008
Sine Institute
2007
Universitätsklinikum Tübingen
2005
University of Tübingen
2005
Institute of Bioinformatics and Systems Biology
2005
Institute of Molecular Biotechnology
2005
Abstract Human mesenchymal stem cells (hMSC) aid in tissue maintenance and repair by differentiating into specialized cell types. Due to this ability, hMSC are currently being evaluated for cell-based therapies of injury degenerative diseases. However, extensive expansion ex vivo is a prerequisite obtain the numbers required human therapy protocols. Recent studies indicate that may contribute cancer development progression either acting as cancer-initiating or through interactions with...
Bispecific antibodies (bsAb) are considered as promising tools for the elimination of disseminated tumour cells in a minimal residual disease situation. The bsAb-mediated recruitment an immune effector cell close vicinity is thought to induce antitumoural response. However, classical bispecific molecules activate only single class that may not yield optimal responses. We therefore constructed intact antibody, BiUII (anti-CD3 × anti-EpCAM), recognizes and T lymphocytes with its two binding...
Glutathione peroxidase 4 (GPX4) is unique as it the only enzyme that can prevent detrimental lipid peroxidation in vivo by reducing peroxides to respective alcohols thereby stabilizing oxidation products of unsaturated fatty acids. During reticulocyte maturation, mediated 15-lipoxygenase humans and rabbits 12/15-lipoxygenase (ALOX15) mice was considered initiating event for elimination mitochondria but now known occur through mitophagy. Yet, genetic ablation Alox15 gene failed provide...
T-cell subsets were studied by fluorescence-activated cell sorter analysis in 57 feline immunodeficiency virus (FIV)-seropositive cats with naturally acquired FIV infection to see whether CD4(+)-CD8+ alterations comparable those observed human virus-infected patients. CD4+ values decreased and CD8+ increased. The CD4+/CD8+ ratio was reduced 1.6, compared 3.3 33 FIV-seronegative control cats. Variance of data showed a significant influence seropositivity, sex, spaying female on values....
Tumor side population (SP) cells display stem-like properties that can be modulated by treatment with the calcium channel blocker verapamil. Verapamil enhance cytotoxic effects of chemotherapeutic drugs and multidrug resistance targeting transport function P-glycoprotein (P-gp). This study focused on therapeutic potential verapamil SP tumor cells, further investigated its chemosensitizing using L3.6pl AsPC-1 pancreatic carcinoma models. As compared to parental (0.9±0.22%),...
Carbonic anhydrase XII (CA XII) is a membrane-tethered cell surface enzyme that highly expressed on many human tumor cells. members in this class of exofacial molecules facilitate metabolism by facilitating CO2 venting and intracellular pH regulation. Accordingly, inhibition CAs has been proposed as general therapeutic strategy to target cancer. The recent characterization 6A10, the first CA XII-specific inhibitory monoclonal antibody, offered an opportunity evaluate with regard XII-mediated...
Dye-effluxing side population (SP) cells can be resistant to chemotherapy and are thought resemble cancer stem cells. We characterized the relevance of SP subpopulation in esophageal cell lines their relation resistance metastasis. The was detected using Hoechst 33342 staining five OE19, OE21, OE33, PT1590, LN1590. CTx-resistant were developed after long-term exposure 5-fluorouracil (5-FU) cisplatin validated by analysis markers, thymidylate synthase ERCC1. While neither LN1590 nor PT1590...
A major goal of tumor immunotherapy is the induction long-lasting systemic T-cell immunity. Bispecific antibodies (bsAbs) that lack immunoglobulin Fc region confer T-cell-mediated killing cells but do not induce long-term memory. In contrast, trifunctional bsAbs comprise an appropriate and, therefore, only recruit T also accessory bear activating Fcγ receptors (FcγR), providing additional T-cell-activating signals and securing presentation tumor-derived antigens to cells. this study, we show...
Mesenchymal stromal cells (MSCs) are released into the airways of preterm infants following lung injury. These display a proinflammatory phenotype and associated with development severe bronchopulmonary dysplasia (BPD). We aimed to characterize functional properties MSCs obtained from tracheal aspirates 50 who required invasive ventilation. Samples were separated by disease severity. The increased proliferative capacity was longer duration mechanical ventilation higher severity BPD....
KREMMER, ELISABETH; THIERFELDER, STEFAN; KUMMER, UDO; LEDERER, RAIMUND; MYSLIWIETZ, JOSEF Author Information
Abstract Background Dendritic cells (DC) pulsed with tumor-derived antigenic material have widely been used in antitumor vaccination protocols. However, the optimal strategy of DC loading has not yet established. Our aim was to define requirements vaccines terms vivo protection a murine B-cell lymphoma model. Methods We compare various reagents including whole parental and modified tumor single tumor-specific antigen, namely idiotype (Id). Bone marrow-derived were vitro for therapy...
Early onset infection (EOI) in preterm infants <32 weeks gestational age (GA) is associated with a high mortality rate and the development of severe acute long-term complications. The pathophysiology EOI not fully understood clinical laboratory signs early infections this patient cohort are often conclusive. Thus, aim study was to identify signatures characterizing by using genome-wide gene expression (GWGE) analyses from umbilical arterial blood infants. This prospective conducted GA. GWGE...
Abstract The current studies were designed to determine the relevance of T cell antigen density, besides antibody isotype, with regard success serotherapy. We compared immunosuppressive effects two rat IgG 2b monoclonal anti‐Thy‐1 antibodies, RmT1 and 30‐H12, distinct binding sites in a graft‐ vs .‐host disease (GVHD) model fully H‐2 I‐A regionmismatched bone marrow transplantation, making use difference Thy‐1.2 density between homozygous (BALB/c) heterozygous (BALB/c×AKR/J)F 1...
A chimeric HLA-DR4-H2-E (DR4) homozygous transgenic mouse line spontaneously develops diverse hematological malignancies with high frequency (70%). The majority of were distributed equally between T and B cell neoplasms included lymphoblastic lymphoma (LTCL), (LBCL), diffuse large (DLBCL), the histiocyte/T rich variant DLBCL (DLBCL-HA/T DLBCL), splenic marginal zone (SMZL), follicular (FBL) plasmacytoma (PCT). Most these highly similar to human diseases. Also, some non-lymphoid such as acute...