A5075076570- Adenosine and Purinergic Signaling
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Neuroscience and Neuropharmacology Research
- Histone Deacetylase Inhibitors Research
- Vagus Nerve Stimulation Research
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Genetic Neurodegenerative Diseases
- Epigenetics and DNA Methylation
- COVID-19 Impact on Reproduction
- Mesenchymal stem cell research
- Long-Term Effects of COVID-19
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Bipolar Disorder and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Organ and Tissue Transplantation Research
- Neuroblastoma Research and Treatments
- Immune cells in cancer
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Developmental Biology and Gene Regulation
- Neuroendocrine Tumor Research Advances
Universidade de São Paulo
2015-2024
Rutgers, The State University of New Jersey
2024
Instituto de Botânica
2012
Background Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) including proliferation stimulation and neural differentiation. Mouse embryonic stem cells (ESC), induced with retinoic acid differentiation, closely assemble processes occurring during neuroectodermal development of early embryo. Principal Findings P2X7R expression together pluripotency marker Oct-4 was highest in undifferentiated ESC. In cells, agonist Bz-ATP accelerated cell cycle entry, which...
Purinergic receptors participate, in almost every cell type, controlling metabolic activities and many physiological functions including signal transmission, proliferation differentiation. While most of P2Y induce transient elevations intracellular calcium concentration by activation pools forward these signals as waves which can also be transmitted into neighboring cells, P2X produce spikes include voltage-operating channels. transients that activate transcription factors responsible for...
One of the challenging problems current radio-chemotherapy is recurrence and metastasis cancer cells that survive initial treatment. We propose one unwanted effects radiochemotherapy release from damaged ("leaky") nucleotides such as ATP UTP exert pro-metastatic functions can directly stimulate chemotaxis cells. To address this problem in a model human lung (LC), we employed several complementary vitro vivo approaches to demonstrate role extracellular (EXNs) LC cell line tumor progression....
Hybrid scaffolds made of xanthan and magnetite nanoparticles (XCA/mag) were prepared by dipping membranes (XCA) into dispersions magnetic for different periods time. The resulting hybrid presented magnetization values ranging from 0.25 emu g−1 to 1.80 at 70 kOe corresponding iron contents 0.25% 2.3%, respectively. They applied as matrices in vitro embryoid body adhesion neuronal differentiation embryonic stem cells; comparison, neat XCA commercial plastic plates also used. Adhesion rates...
Bone marrow metastasis occurs in approximately 350,000 patients that annually die the U.S. alone. In view of importance tumor cell migration into bone marrow, we have here investigated effects various concentrations stromal cell-derived factor-1 (SDF-1), bradykinin- and ATP on metastasis. We show for first time bradykinin augmented chemotactic responsiveness neuroblastoma cells to SDF-1 concentrations, encountered under physiological conditions. Bradykinin upregulated VEGF expression,...
Pharmacological mobilization of hematopoietic stem progenitor cells (HSPCs) from bone marrow (BM) into peripheral blood (PB) is a result mobilizing agent-induced “sterile inflammation” in the BM microenvironment due to complement cascade (ComC) activation. Here we provide evidence that ATP, as an extracellular nucleotide secreted pannexin-1-dependent manner cells, triggers activation ComC and initiates process. This process augmented P2X7 receptor-dependent manner, P2X7-KO mice are poor...
Ectonucleotidases modulate inflammatory responses by balancing extracellular ATP and adenosine (ADO) might be involved in COVID-19 immunopathogenesis. Here, we explored the contribution of nucleotide metabolism to severity mild severe cases disease. We verified that gene expression ectonucleotidases is reduced whole blood patients with negatively correlated levels CRP, an marker disease severity. In line these findings, present higher plasma ADO when compared healthy controls. Cell...
Drug resistance is a major challenge for all oncological treatments that involve the use of cytotoxic agents. Recent therapeutic alternatives cannot circumvent ability cancer cells to adapt or alter natural selection resistant cells, so problem persists. In neuroblastoma, recurrence can occur in up 50% high-risk patients. Therefore, identification novel targets capable modulating survival death following classical antitumor interventions crucial address this problem. study, we investigated...
Introduction Physical exercise has beneficial effects by providing neuroprotective and anti-inflammatory responses to AD. Most studies, however, have been conducted with aerobic exercises, few investigated the of other modalities that also show positive on AD, such as resistance (RE). In addition its benefits in developing muscle strength, balance muscular endurance favoring improvements quality life elderly, RE reduces amyloid load local inflammation, promotes memory cognitive improvements,...
Medulloblastoma (MB) is the most common malignant brain tumor in children and occurs mainly cerebellum. Important intracellular signaling molecules, such those present Sonic Hedgehog Wnt pathways, are involved its development can also be employed to determine grade prognosis. Ectonucleotidases, particularly ecto-5′NT/CD73, important enzymes process of different types regulating extracellular ATP adenosine levels. Here, we investigated activity ectonucleotidases three human cell lines: Daoy...
An unprecedented global health crisis has been caused by a new virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We performed experiments to test if hypertonic saline solution was capable of inhibiting replication. Our data show that 1.2% NaCl inhibited replication 90%, achieving 100% inhibition at 1.5% in the nonhuman primate kidney cell line Vero, and 1.1% sufficient inhibit 88% human epithelial lung Calu-3. Furthermore, our results indicate is due an intracellular...
ABSTRACT During brain development, cells proliferate, migrate and differentiate in highly accurate patterns. In this context, published results indicate that bradykinin functions neural fate determination, favoring neurogenesis migration. However, mechanisms underlying function are yet to be explored. Our findings a previously unidentified role for action inducing neuron-generating division vitro vivo, given lengthened the G1-phase of progenitor (NPC) cycle increased TIS21 (also known as PC3...
Abstract Embryonic carcinoma cells are widely used models for studying the mechanisms of proliferation and differentiation occurring during early embryogenesis. We have now investigated how down‐regulation P2X2 P2X7 receptor expression by RNA interference (RNAi) affects neural phenotype specification P19 embryonal cells. Wild‐type or stably expressing shRNAs targeting were induced to differentiate into neurons glial in presence retinoic acid. Silencing along promoted cell an increase...
The most aggressive subtype of brain tumors is glioma WHO grade IV, the glioblastoma (GBM). present work aims to elucidate role kinin receptors in interactions between GBM cells and mesenchymal stem (MSC). cell line U87-MG was stably transfected express dsRed protein, single cloned, expanded, cultured with MSC, both direct co-cultures (DC) indirect (IC) at equal number ratio for 72 h. Up- down-regulation matrix metalloproteases (MMP)-9 expression MSC cells, respectively, co-culture points...