A5071598003- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Linguistics and Education Research
- RNA Interference and Gene Delivery
- Agricultural and Food Sciences
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Receptor Mechanisms and Signaling
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Adenosine and Purinergic Signaling
- Animal Genetics and Reproduction
- Science and Education Research
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Biotechnology and Related Fields
- Signaling Pathways in Disease
- Graphene and Nanomaterials Applications
- Bone Tissue Engineering Materials
- Academic Research in Diverse Fields
- Ion Channels and Receptors
- 3D Printing in Biomedical Research
- Education Pedagogy and Practices
- Carbon Nanotubes in Composites
Universidade Federal de Minas Gerais
2014-2023
Universidade Federal Fluminense
2018
Bipar
2017
Empresa de Desenvolvimento Mineiro (Portugal)
2017
Toxicologie, Pharmacologie et Signalisation Cellulaire
2014
Universidade Federal de Sergipe
2012
Federal University of São João del-Rei
2009-2012
National Institute of Science and Technology
2012
Instituto de Ensino e Pesquisa Santa Casa
2008-2009
Universidade de São Paulo
2005-2008
Acetylcholine (ACh) has been shown to modulate neuronal differentiation during early development. Both muscarinic and nicotinic acetylcholine receptors (AChRs) regulate a wide variety of physiological responses, including apoptosis, cellular proliferation differentiation. However, the intracellular mechanisms underlying these effects AChR signaling are not fully understood. It is known that activation AChRs increase neurogenesis regulation calcium through may underlie many functions ACh....
Succinate is an intermediate of the citric acid cycle as well extracellular circulating molecule, whose receptor, G protein-coupled receptor-91 (GPR91), was recently identified and characterized in several tissues, including heart. Because some pathological conditions such ischemia increase succinate blood levels, we investigated role this metabolite during a heart ischemic event, using human rodent models. We found that causes cardiac hypertrophy GPR91 dependent manner. activation triggers...
The renin-angiotensin (Ang) system plays a pivotal role in the pathogenesis of cardiovascular disease, with Ang II being major effector this system. Multiple lines evidence have shown that Ang-(1-7) exerts cardioprotective effects heart by counterregulating actions. questions remain are how and where its effects. By using combination molecular biology, confocal microscopy, transgenic rat model increased levels circulating (TGR[A1-7]3292), we evaluated signaling pathways involved...
Heart activity and long-term function are regulated by the sympathetic parasympathetic branches of nervous system. Parasympathetic neurons have received increased attention recently because acetylcholine (ACh) has been shown to play protective roles in heart disease. However, innervation is sparse heart, raising question how cholinergic signaling regulates cardiomyocytes. We hypothesized that non-neuronal secretion ACh from cardiomyocytes plays a role regulation cardiac activity. To test...
Overwhelming evidence supports the importance of sympathetic nervous system in heart failure. In contrast, much less is known about role failing cholinergic neurotransmission cardiac disease. By using a unique genetically modified mouse line with reduced expression vesicular acetylcholine transporter (VAChT) and consequently decreased release acetylcholine, we investigated consequences altered tone for function. M-mode echocardiography, hemodynamic experiments, analysis isolated perfused...
Cholinergic control of the heart is exerted by two distinct branches; autonomic component represented parasympathetic nervous system, and recently described non-neuronal cardiomyocyte cholinergic machinery. Previous evidence has shown that reduced function leads to deleterious effects on myocardium. Yet, whether conditions increased signaling can offset pathological remodeling induced sympathetic hyperactivity, its consequences for these axes are unknown. Here, we investigated models...
Abstract Calcium (Ca 2+ ) is an essential signal transduction element involved in the regulation of several cellular activities and it required at various key stages cell cycle. Intracellular Ca crucial for orderly cycle progression plays a vital role proliferation. Recently, was demonstrated by vitro vivo studies that nucleoplasmic regulates growth. Even though mechanism which nuclear proliferation not completely understood, there are reports demonstrating activation tyrosine kinase...
The tumour mass is composed not only of heterogeneous neoplastic cells, but also a variety other components that may affect cancer cells behaviour. lack detailed knowledge about all the constituents microenvironment restricts design effective treatments. Nerves have been reported to contribute growth and maintenance numerous tissues. effects sensory innervations on remain unclear. Here, by using state-of-the-art techniques, including Cre/loxP technologies, confocal microscopy, in...
Development of RNA interference (RNAi) technology utilizing short interfering sequences (siRNA) has focused on creating methods for delivering siRNAs to cells and enhancing siRNA stability in vitro vivo. Here, we describe a novel approach cellular delivery using coiling into carboxyl-functionalized single-wall carbon nanotubes (SWCNTs). The CNT–siRNA system successfully demonstrates nonspecific toxicity transfection efficiency greater than 95%. This offers the potential different types...
Insulin's metabolic effects in the liver are widely appreciated, but insulin's ability to act as a hepatic mitogen is less well understood. Because insulin receptor (IR) can traffic nucleus, and Ca(2+) signals within nucleus regulate cell proliferation, we investigated whether mitogenic result from activation of Ca(2+)-signaling pathways by IRs nucleus. Insulin-induced increases proliferation depended upon clathrin- caveolin-dependent translocation IR formation inositol 1,4,5,-trisphosphate...