A5049131306- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Trypanosoma species research and implications
- Electrochemical Analysis and Applications
- Cardiomyopathy and Myosin Studies
- Receptor Mechanisms and Signaling
- Atrial Fibrillation Management and Outcomes
- Pesticide Exposure and Toxicity
- Pulmonary Hypertension Research and Treatments
- Neuroscience and Neuropharmacology Research
- Research on Leishmaniasis Studies
- Cardiac Arrhythmias and Treatments
- Coenzyme Q10 studies and effects
- Insect and Pesticide Research
- Cardiac Ischemia and Reperfusion
- Alcoholism and Thiamine Deficiency
- Neuroscience and Neural Engineering
- Cardiovascular Function and Risk Factors
- Adipose Tissue and Metabolism
- Vitamin C and Antioxidants Research
- Cardiovascular Effects of Exercise
- Heart Rate Variability and Autonomic Control
- Neurological and metabolic disorders
- Cancer Treatment and Pharmacology
- Ion Channels and Receptors
Universidade Federal de São Paulo
2015-2025
Brazilian Synchrotron Light Laboratory
2023
Universidade de São Paulo
2014-2022
San Antonio College
2020
Sociedade Brasileira de Cardiologia
2020
CSL (Switzerland)
2020
Hospitais da Universidade de Coimbra
2020
Universidade de Sorocaba
2019
New York Stem Cell Foundation
2017
Universidade Federal de Minas Gerais
2008-2016
Pulmonary arterial hypertension is a devastating disease with high mortality. Familial cases of pulmonary are usually characterized by autosomal dominant transmission reduced penetrance, and some familial have unknown genetic causes.We studied family in which multiple members had without identifiable mutations any the genes known to be associated disease, including BMPR2, ALK1, ENG, SMAD9, CAV1. Three were whole-exome sequencing. Additional patients or idiopathic screened for gene that was...
Morphine is one of the most prescribed and effective drugs used for treatment acute chronic pain conditions. In addition to its central effects, morphine can also produce peripheral analgesia. However, mechanisms underlying this action have not yet been fully elucidated. Here, we show that antinociceptive effect lost in neuronal nitric-oxide synthase null mice induces production nitric oxide primary nociceptive neurons. The activation pathway by was dependent on an initial stimulation...
Overwhelming evidence supports the importance of sympathetic nervous system in heart failure. In contrast, much less is known about role failing cholinergic neurotransmission cardiac disease. By using a unique genetically modified mouse line with reduced expression vesicular acetylcholine transporter (VAChT) and consequently decreased release acetylcholine, we investigated consequences altered tone for function. M-mode echocardiography, hemodynamic experiments, analysis isolated perfused...
Infection with Trypanosoma cruzi induces inflammation, which limits parasite proliferation but may result in chagasic heart disease. Suppressor of cytokine signaling 2 (SOCS2) is a regulator immune responses and therefore participate the pathogenesis T. infection. SOCS2 expressed during infection, its expression partially reduced infected 5-lipoxygenase-deficient [knockout (KO)] mice. In KO mice, there was reduction both parasitemia interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α),...
Heterozygous loss of function mutations in the KCNK3 gene cause hereditary pulmonary arterial hypertension (PAH). encodes an acid-sensitive potassium channel, which contributes to resting potential human artery smooth muscle cells. is widely expressed body, and dimerizes with other subunits, or closely related, KCNK9 channel.We engineered homomeric heterodimeric mutant nonmutant channels associated PAH. Using whole-cell patch-clamp electrophysiology COS7 cell lines, we determined that...
Chagas cardiomyopathy caused by infection with the intracellular parasite
This study was undertaken to elucidate the effect of essential oil from Alpinia speciosa (EOAs) on cardiac contractility and underlying mechanisms. The obtained leaves flowers analyzed by GC-MS method. Chemical analysis revealed presence at least 18 components. Terpinen-4-ol 1,8-cineole corresponded 38% 18% crude oil, respectively. experiments were conducted spontaneously-beating right atria electrically stimulated left isolated adult rats. EOAs isometric contractions frequency in vitro...
Abstract Long QT syndrome (LQTS) is characterized by ventricular arrhythmias and sudden cardiac death. Purkinje cells (PC) within the specialized conduction system have unique electrophysiological properties that we hypothesize may produce primary sources of arrhythmia in heritable LQTS. LQTS type 3 (LQT3) transgenic mice harboring ΔKPQ +/− mutation were crossed with Contactin2-EGFP BAC mice, which express a fluorescent reporter gene fiber network. Isolated myocytes (VMs) (EGFP − ) PCs +...
Chagas Disease (CD) is one of the leading causes heart failure and sudden death in Latin America. Treatments with antioxidants have provided promising alternatives to ameliorate CD. However, specific roles major reactive oxygen species (ROS) sources, including NADPH-oxidase 2 (NOX2), mitochondrial-derived ROS nitric oxide (NO) progression or resolution CD are yet be elucidated. We used C57BL/6 (WT) a gp91PHOX knockout mice (PHOX-/-), lacking functional NOX2, investigate effects ablation...
High serum levels of aldosterone have been linked to the development cardiac disease. In contrast, angiotensin (Ang)-(1-7) was extensively shown possess cardioprotective effects, including attenuation dysfunction induced by excessive mineralocorticoid activation in vivo, suggesting possible interactions between these 2 molecules. Here, we investigated whether there is cross-talk and Ang-(1-7) its functional consequences for calcium (Ca(2+)) signaling ventricular myocytes. Short-term effects...
Loxosceles spider bites cause many human injuries worldwide. Injections in mice of whole (L.) intermedia venom or a recombinant toxin (rLiD1) produce systemic symptoms similar to those detected envenomed humans. This animal model was used characterize the effects cardiac tissues. L. antigens were by ELISA kidney, heart, lung and liver experimentally mice. In addition, rLiD1 binding cardiomyocytes demonstrated immunofluorescence confocal microscopy. Furthermore, isolated perfused heart...
Chagas disease, caused by the triatominae Trypanosoma cruzi, is one of leading causes heart malfunctioning in Latin America. The cardiac phenotype observed 20-30% infected people 10-40 years after their primary infection. complications during disease range from arrhythmias to failure, with important involvement right ventricle. Interestingly, no studies have evaluated electrical properties ventricle myocytes and correlated them parasite persistence. Taking advantage a murine model we studied...
Atrial arrhythmias are a hallmark of heart diseases. The antiarrhythmic drug ranolazine with multichannel blocker properties is promising agent to treat atrial arrhythmias. We therefore used the rat model monocrotaline-induced pulmonary-hypertension assess whether can reduce incidence ex vivo in isolated right atrium. Four-week-old Wistar rats were injected 50 mg/kg monocrotaline, and atrium function was studied 14 days later. developed ventricular hypertrophy, ECG showed an increased P wave...