A5043529938- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Cardiomyopathy and Myosin Studies
- Moringa oleifera research and applications
- Mesenchymal stem cell research
- Science and Education Research
- Tissue Engineering and Regenerative Medicine
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Synthesis and Biological Evaluation
- Parasites and Host Interactions
- Effects of Radiation Exposure
- Respiratory Support and Mechanisms
- Signaling Pathways in Disease
- Liver physiology and pathology
- Cardiac electrophysiology and arrhythmias
- Calcium signaling and nucleotide metabolism
- Education during COVID-19 pandemic
- Cardiovascular Function and Risk Factors
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Advanced Glycation End Products research
- Cardiac Fibrosis and Remodeling
- Parasitic Diseases Research and Treatment
- Polymer Nanocomposite Synthesis and Irradiation
- Spinal Cord Injury Research
National Institute of Science and Technology for Structural Biology and Bioimaging
2016-2025
Universidade Federal do Rio de Janeiro
2015-2025
University of Miami
2025
Hospital Universitário Clementino Fraga Filho
2014-2017
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2014-2017
Instituto de Biofísica Carlos Chagas Filho
2015-2016
Universidade Federal Fluminense
2012
Chronic chagasic cardiomyopathy (CCC) develops years after acute infection by Trypanosoma cruzi and does not improve trypanocidal therapy, despite reduction of parasite burden. During disease, the heart undergoes oxidative stress, a potential causative factor for arrhythmias contractile dysfunction. Here we tested whether antioxidants/ cardioprotective drugs could cardiac function in established Chagas disease. We chose model that resembles B1-B2 stage human CCC, treated mice with...
TGF-β involvement in Chagas disease cardiomyopathy has been clearly demonstrated. The signaling pathway is activated the cardiac tissue of chronic phase patients and associated with an increase extracellular matrix protein expression. aim this study was to investigate effect GW788388, a selective inhibitor TβR1/ALK5, on function experimental model Chagas' heart disease. To end, C57BL/6 mice were infected Trypanosoma cruzi (102 parasites from Colombian strain) treated orally 3mg/kg GW788388...
Infection with Trypanosoma cruzi induces inflammation, which limits parasite proliferation but may result in chagasic heart disease. Suppressor of cytokine signaling 2 (SOCS2) is a regulator immune responses and therefore participate the pathogenesis T. infection. SOCS2 expressed during infection, its expression partially reduced infected 5-lipoxygenase-deficient [knockout (KO)] mice. In KO mice, there was reduction both parasitemia interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α),...
This study aimed to investigate the pathophysiology of hepatic microcirculatory dysfunction in non-alcoholic fatty liver disease (NAFLD).In Wistar rats, NAFLD model was induced by 20 weeks high-fat diet (HFD) feeding. Rolling and adhesion leukocytes tissue perfusion microcirculation were examined using vivo microscopic laser speckle contrast imaging (LSCI), respectively. Oxidative stress inflamatory parameters analysed TBARs, catalase enzyme activity, RT-PCR ELISA. The participation advanced...
CCL3, a member of the CC-chemokine family, has been associated with macrophage recruitment to heart tissue and parasite control in acute infection mouse Trypanosoma cruzi, causative agent Chagas disease. We approached participation CCL3 chronic chagasic cardiomyopathy (CCC), main clinical form induced CCC C57BL/6 (ccl3+/+) CCL3-deficient (ccl3-/-) mice by Colombian strain. In chronically infected ccl3+/+ mice, high levels mRNA protein were detected tissue. Survival was not affected...
Background Chronic chagasic cardiomyopathy (CCC), the main clinical sign of Chagas disease, is associated with systemic CD8+ T-cell abnormalities and CD8-enriched myocarditis occurring in an inflammatory milieu. Pentoxifylline (PTX), a phosphodiesterase inhibitor, has immunoregulatory cardioprotective properties. Here, we tested PTX effects on cardiac alterations using model experimental Chagas' heart disease. Methodology/Principal Findings C57BL/6 mice chronically infected by Colombian...
American tegumentary leishmaniasis (ATL) and visceral (VL) are considered neglected by the World Health Organization. VL can be lethal if not treated; drugs used in treatment toxic, there cases of resistance. Preclinical tests represent a bottleneck discovering new medicines for treatment, depending on animal model, strain used, inoculum route. The golden hamster stands out its high susceptibility to subgenera Viannia Leishmania species, displaying many clinical immunopathological processes...
Abstract Immune cell separation from heterogeneous samples is crucial for various downstream research applications. Standard methods purification are laborious and require additional fluorescent or magnetic labels to identify cells of interest. Approaches contingent upon the use may interfere with testing analysis, like single sequencing oncology research. Label-free sorting by electrokinetic dielectrophoresis (DEP) has recently been used characterize separate target subpopulations complex...
This is a pilot clinical study primarily designed to assess the feasibility and safety of X-ray-guided percutaneous intraspinal injection allogeneic canine adipose tissue-derived mesenchymal stem cells in dogs with chronic spinal cord injury. Six paraplegia (≥six months) were intraparenchymally injected site lesion. Cells obtained from subcutaneous tissue healthy dog, cultured passage 3, labeled 99mTechnetium, transplanted into lesion by injection. Digital X-ray efficiently guided cell as...
Heart failure represents an important public health issue due to its high costs and growing incidence worldwide. Evidence showing the regenerative potential of postmitotic heart tissue has suggested existence endogenous cardiac stem cells in adult hearts. Cardiosphere-derived (CDC) constitute a candidate pool such cells. Previous studies using acute myocardial infarction (MI) models rodents demonstrated improvement function after cell therapy with CDC. We evaluated therapeutic CDC 60 days MI...
Chagasic chronic cardiomyopathy (CCC) is the primary clinical manifestation of Chagas disease (CD), caused by Trypanosoma cruzi. Current therapeutic options for CD are limited to benznidazole (Bz) and nifurtimox. Amiodarone (AMD) has emerged as most effective drug treating arrhythmic form CCC. To address effects Bz AMD we used a preclinical model Female C57BL/6 mice were infected with T. cruzi subjected oral treatment 30 consecutive days, either monotherapy or in combination. decreased...
Abstract Introduction The objective of this work was to evaluate the efficacy placenta-derived mesenchymal stem cell (MSC) therapy in a mouse model myocardial infarction (MI). Since MSCs can be obtained from two different regions human term placenta (chorionic plate or villi), cells both these were compared so that best candidate for could selected. Methods For vitro studies, chorionic (cp-MSCs) and villi (cv-MSCs) extensively characterized their genetic stability, clonogenic differentiation...
Background Chagas disease, caused by the protozoan Trypanosoma cruzi (T.cruzi), is a complex disease endemic in Central and South America. It has been gathering interest due to increases non-vectorial forms of transmission, especially developed countries. The objective this work was investigate if adipose tissue-derived mesenchymal stromal cells (ASC) can alter course attenuate pathology mouse model chagasic cardiomyopathy. Methodology/Principal Findings ASC were injected intraperitoneally...
This study aimed to investigate radiation-induced lesions on the skin in an experimental animal model. Methods and Materials: Cutaneous wounds were induced Wistar rats by 4 MeV energy electron beam irradiation, using a dose rate of 240 cGy/min, for 3 different doses (10 Gy, 40 60 Gy). The was observed 5, 10, 25 days (D) after ionizing radiation exposition.Infiltrate inflammatory process D5 D10, Gy groups, progressive increase transforming growth factor β1 is associated with this process. It...
Objectives: Sepsis is associated with cardiac contractile dysfunction attributed to alterations in Ca 2+ handling. We examined the subcellular mechanisms involved sarcoplasmic reticulum loss that mediate altered handling and sepsis. Design: Randomized controlled trial. Setting: Research laboratory Subjects: Male wild type transgenic mice Interventions: induced sepsis using colon ascendens stent peritonitis model. Measurements Main Results: Twenty-four hours after surgery, we observed had...
Background/Aims: Thyroid hormone (TH) signalling is critical for heart function. The expresses thyroid receptors (THRs); THRα1 and THRβ1. We aimed to investigate the regulation mechanisms of THRβ isoform, its association with gene expression changes implications cardiac Methods: experiments were performed using adult male mice expressing TRβΔ337T, which contains Δ337T mutation human THRB impairs ligand binding. Cardiac function RNA studied after hypo-or hyperthyroidism inductions. T3-induced...
Chagas disease (CD), caused by the protozoan Trypanosoma cruzi , is a neglected tropical and health problem in Latin America. Etiological treatment has limited effectiveness chronic CD; thus, new therapeutic strategies are required. The practice of physical exercises been widely advocated to improve quality life CD patients. most frequent clinical manifestation indeterminate form (CIF), effect on progression remains unknown. Here, CIF model, we aimed evaluate cardiac histological,...
Doxorubicin (Dox) is a chemotherapy drug with limited application due to cardiotoxicity that may progress heart failure. This study aims evaluate the role of cardiomyocytes derived from mouse embryonic stem cells (CM-mESCs) in treatment Dox-induced cardiomyopathy (DIC) mice. The cell (mESC) line E14TG2A was characterized by karyotype analysis, gene expression using RT-PCR and immunofluorescence. Cells were transduced luciferase 2 submitted cardiac differentiation. Total conditioned medium...
Emphysema is characterized by loss of lung tissue elasticity and destruction structures supporting alveoli capillaries. The impact mechanical ventilation strategies on ventilator-induced injury (VILI) in emphysema poorly defined. New ventilator should be developed to minimize VILI emphysema. present study was divided into two protocols: 1) characterization an elastase-induced model rats identification the time point greatest cardiorespiratory impairment, defined as a high specific elastance...
In patients with emphysema, invasive mechanical ventilation settings should be adjusted to minimize hyperinflation while reducing respiratory effort and providing adequate gas exchange. We evaluated the impact of pressure-controlled (PCV) pressure support (PSV) on pulmonary diaphragmatic damage, as well cardiac function, in experimental emphysema. Emphysema was induced by intratracheal instillation porcine pancreatic elastase Wistar rats, once weekly for 4 weeks. Control animals received...
Toxoplasmosis is a globally significant disease that poses severe threat to immunocompromised individuals, especially in Brazil, where high prevalence of virulent and atypical strains Toxoplasma gondii observed. In 1998, the EGS strain, exhibiting unique infection phenotype, was isolated adding complexity strain diversity. The P2X7 receptor critical inflammation controlling intracellular microorganisms such as T. gondii. However, its genetic variability can result dysfunction, potentially...
Background: The failure to translate preclinical results the clinical setting is rule, not exception. One reason that frequently overlooked whether animal model reproduces distinctive features of human disease. Another reproducibility method used measure treatment effects in studies. Left ventricular (LV) function improvement most common endpoint cardiovascular disease studies, while echocardiography evaluate LV function. In this work, we conducted a robust echocardiographic evaluation size...