A5040593914- Mesenchymal stem cell research
- Adenosine and Purinergic Signaling
- Pituitary Gland Disorders and Treatments
- Mosquito-borne diseases and control
- Tissue Engineering and Regenerative Medicine
- Liver Disease Diagnosis and Treatment
- Parasites and Host Interactions
- Growth Hormone and Insulin-like Growth Factors
- Research on Leishmaniasis Studies
- Viral Infections and Vectors
- Proteoglycans and glycosaminoglycans research
- Liver physiology and pathology
- Wound Healing and Treatments
- Chronic Kidney Disease and Diabetes
- Acute Kidney Injury Research
- Galectins and Cancer Biology
- Neuroendocrine Tumor Research Advances
- Adipose Tissue and Metabolism
- Trypanosoma species research and implications
- Adipokines, Inflammation, and Metabolic Diseases
- Angiogenesis and VEGF in Cancer
- Respiratory Support and Mechanisms
- Ion Transport and Channel Regulation
- Peptidase Inhibition and Analysis
- Adrenal and Paraganglionic Tumors
Universidade Federal do Rio de Janeiro
2016-2025
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2013-2023
Hospital Universitário Clementino Fraga Filho
1993-2023
University of Verona
2016-2020
Zero to Three
2020
Faculdade de Medicina de Petrópolis
2020
Universidade do Estado do Rio de Janeiro
2004-2018
Instituto de Biofísica Carlos Chagas Filho
2011-2018
Czech Academy of Sciences, Institute of Biophysics
2015
Fundação Carlos Chagas
2015
The novel coronavirus SARS-CoV-2 causes COVID-19, a highly pathogenic viral infection threatening millions. majority of the individuals infected are asymptomatic or mildly symptomatic showing typical clinical signs common cold. However, approximately 20% patients can progress to acute respiratory distress syndrome (ARDS), evolving death in about 5% cases. Recently, angiotensin-converting enzyme 2 (ACE2) has been shown be functional receptor for virus entry into host target cells....
The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to stem cells or linked other components adipose tissue. This work aimed at analysis histologic and ultrastructural changes aged facial skin after injection graft in addition its stromal vascular fraction, obtained by centrifugation, compare with those expanded adipose-derived mesenchymal cells.This study was performed six consecutive patients who were...
Abstract Clearance of apoptotic exudate neutrophils (efferocytosis) induces either pro- or anti-inflammatory responses in mouse macrophages depending on host genetic background. In this study, we investigated whether neutrophil efferocytosis a stable macrophage phenotype that could be recalled by late restimulation with LPS. Bone marrow-derived previously stimulated but not expressed regulatory/M2b characterized low IL-12 and high IL-10 production following restimulation, increased...
The objective of our study was to evaluate the therapeutic potential bone marrow mesenchymal stromal cells (MSC) in a rat model severe chronic liver injury. Fourteen female Wistar rats were fed exclusively an alcoholic liquid diet and received intraperitoneal injections carbon tetrachloride every other day during 15 weeks. After this period, eight animals (MSC group) had 1 x 10(7) injected into portal vein while six (placebo vehicle. Blood analysis performed alanine aminotransferase (ALT),...
In a previous study, the authors demonstrated that treatment with expanded adipose-derived stem cells or stromal vascular fraction (SVF)-enriched fat modify pattern of dermis in human beings, representing skin rejuvenation effect. Considering require cell factor, wanted to assess similar results by replacing them platelet-rich plasma (PRP), which is easier obtain and for an empirical regenerative effect has been already described. To determine if PRP injection could replace cutaneous cells....
The effects of capsular polysaccharides, galactoxylomannan (GalXM) and glucuronoxylomannan (GXM), from acapsular (GXM negative) encapsulate strains Cryptococcus neoformans were investigated in RAW 264.7 peritoneal macrophages. Here, we demonstrate that GalXM GXM induced different cytokines profiles production TNF-α, NO iNOS expression, while predominantly TGF-β secretion. Both early morphological changes identified as autophagy late macrophages apoptosis mediated by Fas/FasL interaction, a...
Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, subendosteal niche is its cellular composition role each cell population in fate have not been established. In vivo models involve subtle species-specific differences, while bidimensional cultures do reflect 3D tissue organization. The aim this study was to investigate vitro human...
Drug resistance, a process mediated by multiple mechanisms, is critical determinant for treating lung cancer. The aim of this study to determine if oleanolic acid (OA), pentacyclic triterpene present in several plants, able circumvent the mechanisms drug resistance non-small cell cancer (NSCLC) lines and induce their death.OA decreased viability NSCLC A459 H460 despite presence active, multidrug-resistant (MDR) MRP1/ABCC1 proteins anti-apoptotic Bcl-2 survivin. These effects are due...
Silicosis is an occupational lung disease with no effective treatment. We hypothesized that dasatinib, a tyrosine kinase inhibitor, might exhibit therapeutic efficacy in silica-induced pulmonary fibrosis. was induced C57BL/6 mice by single intratracheal administration of silica particles, whereas the control group received saline. After 14 days, when already established, animals were randomly assigned to receive DMSO or dasatinib (1 mg/kg) oral gavage, twice daily, for days. On day 28,...
The therapeutic potential of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) in kidney injury has been largely reported. However, new approaches are necessary to optimize the efficacy treatment renal diseases. MSCs physiologically under a low O2 partial pressure (pO2), and culturing adipose-derived (ADMSCs) hypoxia alters their secretory paracrine properties. aim this study was evaluate whether preconditioning ADMSCs properties secreted EVs improve recovery after...
Patients with germline AIP mutations or low protein expression have large, invasive somatotroph adenomas and poor response to somatostatin analogues (SSA).To study the mechanism of 31 sporadic somatotropinomas (n = 13) high 18) were analyzed for messenger RNA (mRNA) 11 microRNAs (miRNAs) predicted bind 3'UTR AIP. Luciferase reporter assays wild-type deletion constructs AIP-3'UTR used effect selected miRNAs in GH3 cells. Endogenous mRNA levels measured after miRNA over- underexpression HEK293...
Silicosis is an occupational lung disease, characterized by irreversible and progressive fibrosis. Silica exposure leads to intense inflammation, reactive oxygen production, extracellular ATP (eATP) release macrophages. The P2X7 purinergic receptor thought be important immunomodulator that responds eATP in sites of inflammation tissue damage. present study investigates the role a murine model silicosis. To end wild-type (C57BL/6) knockout mice received intratracheal injection saline or...
<i>Background:</i> Some pituitary adenomas exhibit fast growth and invade surrounding structures. To date, there is no robust marker to predict invasiveness. <i>Aim:</i> evaluate Ki-67, p53 aryl hydrocarbon receptor-interacting protein (AIP) expression compare these between invasive noninvasive somatotropinomas nonfunctioning (NFPAs). <i>Methods:</i> Protein was determined by immunohistochemistry. Tumors were classified according percentage of...
Only one study has evaluated Ki-67 as a predictor of the response to somatostatin analog therapy in acromegaly; however, other predictors like receptor type 2 (SSTR2) and cytokeratin pattern expressions were not considered.To evaluate whether is octreotide LAR (OCT-LAR) somatotropinomas independent SSTR2 expression patterns.Protein was analyzed by immunohistochemistry. The percentage cell nuclei that immunolabeled for cells with positive staining calculated. considered high when ≥25%, cutoff...
ATP is released in response to cellular damage, and P2X7 receptors have an essential role the onset maintenance of pathological changes. Haemorrhagic cystitis (HC) a well-known adverse effect therapy with cyclophosphamide used for treatment many solid tumours autoimmune conditions. Here we evaluated model HC induced by cyclophosphamide.Effects pharmacological antagonism or genetic deletion receptor on cyclophosphamide-induced mice was assessed nociceptive inflammatory measures. In addition,...
We investigated the regenerative capacity of intravenous administration bone marrow-derived mononuclear cells (BMMCs) in a rat model bilateral renal ischemia/reperfusion (IR) injury and involvement inflammatory anti-inflammatory other biological markers this process.Rats were subjected to 1h pedicle clamping. BMMCs injected i.v after reperfusion tracked by 99mTc GFP+ BMMCs. Twenty-four hours reperfusion, function histological changes evaluated. The mRNA (real time PCR) protein (ELISA...
There are few data regarding ZAC1 expression in clinically non-functioning pituitary adenomas (NFPA). Because somatotropinomas and NFPA behave differently with respect to tumor shrinkage during somatostatin analogs (SA) therapy, we sought compare the receptor (sstr) types 1, 2, 3 5 mRNA these two adenoma subtypes normal human pituitaries.ZAC1 SSTR levels were evaluated using real-time RT-PCR (TaqMan) 20 compared 23 five pituitaries. The invasiveness was magnetic resonance imaging Hardy's...