A5055981133- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
- Research on Leishmaniasis Studies
- Fungal Infections and Studies
- Drug Transport and Resistance Mechanisms
- Antifungal resistance and susceptibility
- Carbohydrate Chemistry and Synthesis
- Piperaceae Chemical and Biological Studies
- COVID-19 Clinical Research Studies
- Galectins and Cancer Biology
- Synthesis and Biological Evaluation
- Nail Diseases and Treatments
- Biochemical and Molecular Research
- Toxin Mechanisms and Immunotoxins
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Inflammasome and immune disorders
- RNA modifications and cancer
- Insect Pest Control Strategies
- Long-Term Effects of COVID-19
- Cancer therapeutics and mechanisms
- Cell Adhesion Molecules Research
- Traditional and Medicinal Uses of Annonaceae
- Cancer Research and Treatments
- Synthesis and biological activity
Universidade Federal do Rio de Janeiro
2016-2025
Instituto de Biofísica Carlos Chagas Filho
2004-2023
Fundação Oswaldo Cruz
2023
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2012-2021
University of Washington
2009-2012
Pacific Northwest Diabetes Research Institute
2009-2012
Fundação Carlos Chagas
2007-2010
Universidade Federal Rural do Rio de Janeiro
2004
The novel coronavirus SARS-CoV-2 causes COVID-19, a highly pathogenic viral infection threatening millions. majority of the individuals infected are asymptomatic or mildly symptomatic showing typical clinical signs common cold. However, approximately 20% patients can progress to acute respiratory distress syndrome (ARDS), evolving death in about 5% cases. Recently, angiotensin-converting enzyme 2 (ACE2) has been shown be functional receptor for virus entry into host target cells....
The process termed “epithelial–mesenchymal transition” (EMT) was originally discovered in ontogenic development, and has been shown to be one of the key steps tumor cell progression metastasis. Recently, we showed that expression some glycosphingolipids (GSLs) is down-regulated during EMT human mouse lines. Here, demonstrate involvement GalNAc-type (or mucin-type) O-glycosylation process, induced with transforming growth factor β (TGF-β) prostate epithelial We found that: ( i ) TGF-β...
Growing evidences indicate that aberrant glycosylation can modulate tumor cell invasion and metastasis. The process termed "epithelial-mesenchymal transition" (EMT) provides a basic experimental model to shed light on this complex process. EMT involves striking decline in epithelial markers, accompanied by enhanced expression of mesenchymal culminating morphology change increased motility. Few recent studies have established the participation during EMT. Studies now come into knowledge...
Multidrug resistance (MDR) in cancer arises from cross-resistance to structurally- and functionally-divergent chemotherapeutic drugs. In particular, MDR is characterized by increased expression activity of ATP-binding cassette (ABC) superfamily transporters. Sphingolipids are substrates ABC proteins cell signaling, membrane biosynthesis, inflammation, for example, their products can favor progression. Glucosylceramide (GlcCer) a ubiquitous glycosphingolipid (GSL) generated glucosylceramide...
Upon activation, cytotoxic CD8(+) T lymphocytes are desialylated exposing beta-galactose residues in a physiological change that enhances their effector activity and can be monitored on the basis of increased binding lectin peanut agglutinin. Herein, we investigated impact sialylation mediated by trans-sialidase, specific unique Trypanosoma transglycosylase for sialic acid, cell response mice infected with T. cruzi. Our data demonstrate cruzi uses its trans-sialidase enzyme to resialylate...
The work reported herein describes the synthesis and assessment of trypanocidal activity thirteen new 1,2,4-triazole-3-thiones obtained from natural piperine, main constituent dry fruits Piper nigrum. It is part a research program aiming to use abundant easily available products as starting materials for design molecules potentially useful antiparasitic drugs. variously substituted triazole derivatives were synthesized amide in four steps with microwave irradiation on overall yields ranging...
Chagas cardiomyopathy is the most severe clinical manifestation of chronic disease. The disease affects Latin American countries, being considered one leading causes morbidity and death in continent. pathogenesis very complex, with mechanisms involving parasite-dependent cytopathy, immune-mediated myocardial damage neurogenic disturbances. These pathological changes eventually result cardiac myocyte hypertrophy, arrhythmias, congestive heart failure stroke during infection phase. Herein, we...
Commonly found at the outermost ends of complex carbohydrates in extracellular medium or on outer cell membranes, sialic acids play important roles a myriad biological processes. Mammals synthesize acid through pathway, but Trypanosoma cruzi, agent Chagas' disease, evolved to obtain from its host trans-sialidase (TcTS) reaction. Studies parasite surface architecture and biochemistry indicate that unique system comprising sialoglycoproteins sialyl-binding proteins assists several functions...
Background: Piperine, an amide extracted from the Piper spices, exhibits strong anti-tumor properties. However, its effect on epithelial–mesenchymal transition (EMT) process has never been investigated. Herein, we evaluate toxic of piperine lung adenocarcinoma (A549), breast (MDA-MB-231) and hepatocellular carcinoma (HepG2) cell lines, as well ability to inhibit EMT-related events induced by TGF-β1 treatment. Methods: The viability was investigated MTT assay. Protein expression evaluated...
Abstract Background Coronavirus disease 2019 (COVID-19) can progress to severe pneumonia with respiratory failure and is aggravated by the deregulation of immune system causing an excessive inflammation including cytokine storm. Methods In this study, we report that acutely infected patients have high levels both type-1 type-2 cytokines. Results Our results show abnormal upon T-cell stimulation, in a nonpolarized profile. Furthermore, our findings indicate hyperactive response associated...
Cryptococcosis is a disease caused by the pathogenic fungi Cryptococcus neoformans and gattii, both environmental that cause severe pneumonia may even lead to cryptococcal meningoencephalitis. Although C. affects more fragile individuals, such as immunocompromised hosts through opportunistic infections, gattii causes serious indiscriminate primary infection in immunocompetent individuals. Typically seen tropical subtropical environments, has increased its endemic area over recent years,...
Multidrug resistance (MDR) and induction of metastasis are some the puzzles encountered during cancer chemotherapy. The MDR phenotype is associated with overexpression ABC transporters, involved in drug efflux. Metastasis originates from epithelial-mesenchymal transition (EMT), which cells acquire a migratory phenotype, invading new tissues. transporters' role EMT still elusive, though undergoing exhibit enhanced ABCB1 expression. We demonstrated increased expression but no change activity...
Cryptococcus neoformans is a lethal fungus that primarily affects the respiratory system and central nervous system. One of main virulence factors capsule, constituted by polysaccharides glucuronoxylomannan (GXM) glucuronoxylomanogalactan (GXMGal). Polysaccharides are immunomodulators. target cell populations for modulation macrophages, which part first line defense important innate adaptive immunity. It has been reported macrophages can be modulated to act as “Trojan horse,” taking...
The protozoan responsible for Chagas' disease, Trypanosoma cruzi, expresses on its surface an unusual trans-sialidase enzyme thought to play important role in host–parasite interactions. Trans-sialidase is the product of a multigene family encoding both active and inactive proteins. We have demonstrated that despite lacking enzymatic activity due single mutation, Tyr342-His, displays sialic acid binding activity, with identical specificity analogue. In this work we demonstrate recombinant...