A5059344700- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Fungal Infections and Studies
- Polysaccharides and Plant Cell Walls
- Piperaceae Chemical and Biological Studies
- Osteoarthritis Treatment and Mechanisms
- Synthesis and Biological Evaluation
- Sphingolipid Metabolism and Signaling
- Proteoglycans and glycosaminoglycans research
- Antifungal resistance and susceptibility
- Enzyme Catalysis and Immobilization
- Traditional and Medicinal Uses of Annonaceae
- Biofuel production and bioconversion
- HIV/AIDS drug development and treatment
- Studies on Chitinases and Chitosanases
- Adenosine and Purinergic Signaling
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- Insect symbiosis and bacterial influences
- Toxoplasma gondii Research Studies
- Macrophage Migration Inhibitory Factor
Universidade Federal do Rio de Janeiro
2005-2024
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2018
Fundação Carlos Chagas
2002-2017
Instituto de Biofísica Carlos Chagas Filho
2002-2017
University of Oklahoma Health Sciences Center
2009
Universidade Federal Rural do Rio de Janeiro
2004
Universidade Federal de Santa Catarina
2003
de Duve Institute
1999-2000
UCLouvain
1999-2000
KU Leuven
1997
In the present study, we characterized in vitro modulation of NETs (neutrophil extracellular traps) induced human neutrophils by opportunistic fungus Cryptococcus neoformans, evaluating participation capsular polysaccharides glucuronoxylomanan (GXM) and glucuronoxylomannogalactan (GXMGal) this phenomenon. The mutant acapsular strain CAP67 polysaccharide GXMGal NET production. contrast, wild-type major GXM did not induce release. addition, C. neoformans inhibited PMA-induced Additionally,...
Abstract Hyperglycemia is a common feature of diabetes mellitus, considered as risk factor for cancer. However, its direct effects in cancer cell behavior are relatively unexplored. Herein we show that high glucose concentration induces aberrant glycosylation, increased proliferation, invasion and tumor progression colon By modulating the activity rate-limiting enzyme, glutamine-fructose-6-phosphate amidotransferase (GFAT), demonstrate hexosamine biosynthetic pathway (HBP) involved those...
We investigated early cellular responses induced by infection with Leishmania major in macrophages from resistant C57/BL6 mice. Infection increased production of reactive oxygen species resident, but not inflammatory peritoneal macrophages. In addition, activation stress-activated protein kinases/c-Jun N-terminal kinases (SAPK/JNK) also expression membrane and soluble FasL, infected remained viable after 48 h. secretion cytokines/chemokines TNF-α, IL-6, TIMP-1, IL-1RA, G-CSF, TREM, KC,...
ABSTRACT The increased resistance of the protozoan parasite Trypanosoma cruzi to nitro derivatives is one major problems for successful treatment Chagas' disease. In present study, we have tested effects 1- O -hexadecylphosphocholine (miltefosine) against strains T. that are partially resistant (strain Y) and highly Colombiana) drugs in clinical use. As expected, epimastigotes strain Colombiana showed higher levels benznidazole than those Y. However, level miltefosine was same both strains....
The survival of Trypanosoma cruzi, the causative agent Chagas' disease, depends vitally on proteins and glycoconjugates that mediate parasite/host interaction. Since most these molecules are attached to membrane by glycosylphosphatidylinositol (GPI), alternative means chemotherapeutic intervention might emerge from GPI biosynthesis studies. structure major 1G7 antigen has been fully characterized us (Güther, M. L. S., Cardoso de Almeida, L., Yoshida, N., Ferguson, A. J.(1992) J. Biol. Chem....
Background The yolk of insect eggs is a cellular domain specialized in the storage reserve components for embryo development. macromolecules are stored different organelles and their interactions with cells mostly unknown. Acidocalcisomes lysosome-related characterized by acidic nature, high electron density large content polyphosphate bound to several cations. In this work, we report presence acidocalcisome-like vector Rhodnius prolixus. Methodology/Principal findings Characterization...
Complex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. and an acapsular mutant (Cap67). The structures the GIPCs were determined by combination tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass chemical degradation. main GIPC WT had structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl...
IPC (inositol phosphorylceramide) synthase is an enzyme essential for fungal viability, and it the target of potent antifungal compounds such as rustmicin aureobasidin A. Similar to fungi some other lower eukaryotes, protozoan parasite Trypanosoma cruzi capable synthesizing free or protein-linked glycoinositolphospholipids containing IPC. As a first step towards understanding importance mechanism synthesis in T. cruzi, we investigated effects A on proliferation different life-cycle stages...
This review highlights the chemical structures of complex glycoconjugates from protozoan and helminth parasites, etiologic agents major world-wide infections. Several studies on parasitic diseases indicate that glycan portions linked to proteins or lipids, expressed cell surface secreted by protozoa Trypanosoma, Leishmania, trematodes nematodes are virulence determinants responsible for host-parasite interaction immunomodulation in infected animals humans. Also, unique carbohydrate moieties...
The structure of an exopolysaccharide (EPS) produced by Burkholderia brasiliensis , a diazotrophic endophytic organism originally isolated from rice roots, has been determined. bacterium was grown in synthetic medium, containing mannitol and glutamate, which favours the expression two anionic EPSs, were separated anion‐exchange chromatography. repeat unit EPS A, eluted at higher ionic strength, determined combination methylation analysis, partial hydrolysis, chemical degradations, NMR...
Trypanosoma cruzi, the causative agent of Chagas disease, is surrounded by a mucin coat that plays important functions in parasite survival/invasion and extensively O-glycosylated Golgi cell surface glycosyltransferases. The addition first sugar, α-N-acetylglucosamine (GlcNAc) linked to Threonine (Thr), catalyzed polypeptide α-GlcNAc-transferase (pp-αGlcNAcT) which unstable purification. Here, comparison genomes T. cruzi Dictyostelium discoideum, an amoebazoan also forms this linkage,...
The protozoan parasite Trypanosoma cruzi is the causative agent of human Chagas disease, for which there currently no cure. life cycle T. complex, including an extracellular phase in triatomine insect vector and obligatory intracellular stage inside vertebrate host. These phases depend on a variety surface glycosylphosphatidylinositol-(GPI-) anchored glycoconjugates that are synthesized by parasite. Therefore, expression GPI-anchored components biosynthetic pathways GPI anchors attractive...
Previous work from our group showed that tamoxifen, an oral drug has been in use for the treatment of breast cancer over 40 years, is active both vitro and vivo against several species Leishmania, etiological agent leishmaniasis. Using a combination metabolic labeling with [3H]-sphingosine myo-[3H]-inositol, alkaline hydrolysis, HPTLC fractionations mass spectrometry analyses, we observed perturbation metabolism inositolphosphorylceramides (IPCs) phosphatidylinositols (PIs) after L....
Novel structures of glycoinositolphosphorylceramide (GIPC) from the infective yeast form Sporothrix schenckii were determined by methylation analysis, mass spectrometry and NMR spectroscopy. The lipid portion was characterized as a ceramide composed C‐18 phytosphingosine N‐acylated either 2‐hydroxylignoceric acid (80%), lignoceric (15%) or 2,3‐dihydroxylignoceric acids (5%). linked through phosphodiester to myo ‐inositol (Ins) which is substituted on position O‐6 an oligomannose chain....