A5048635362- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Toxoplasma gondii Research Studies
- Microtubule and mitosis dynamics
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Protist diversity and phylogeny
- Cellular transport and secretion
- Trypanosoma species research and implications
- Polysaccharides and Plant Cell Walls
- Endoplasmic Reticulum Stress and Disease
- Biocrusts and Microbial Ecology
- Slime Mold and Myxomycetes Research
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Parasitic Infections and Diagnostics
- Heme Oxygenase-1 and Carbon Monoxide
- Monoclonal and Polyclonal Antibodies Research
- Force Microscopy Techniques and Applications
- Muscle Physiology and Disorders
- Lysosomal Storage Disorders Research
- Skin and Cellular Biology Research
- Herpesvirus Infections and Treatments
University of Georgia
2015-2025
University of Kansas Medical Center
2024
University of California, Merced
2024
University of Oklahoma Health Sciences Center
2009-2022
University of Strathclyde
2021
University of Oklahoma
2009-2014
University of Nebraska–Lincoln
2010
University of Oklahoma Medical Center
2006
Florida College
1983-2004
University of Florida
1994-2004
Abstract Background The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores stalk cells. There four groups dictyostelids, the most derived being contains model species Dictyostelium discoideum . Results We have produced draft genome sequence another dictyostelid, purpureum , compare it to D. genome. assembly (8.41 × coverage) comprises 799 scaffolds...
In chronic lymphocytic leukemia (CLL), the lymph node (LN) microenvironment delivers critical survival signals by inducing expression of anti-apoptotic Bcl-2 members Bcl-XL, Bfl-1, and Mcl-1, resulting in apoptosis blockade. We determined previously that resistance against various drugs, among which is clinically applied BH3 mimetic venetoclax, dominated upregulation regulator Bcl-XL. Direct clinical targeting Bcl-XL by, e.g., Navitoclax however not desirable due to induction...
Toxoplasma bradyzoites reside in tissue cysts that undergo cycles of expansion, rupture, and release to foster chronic infection. The glycosylated cyst wall acts as a protective barrier, although the processes responsible for formation, remodeling, turnover are not understood. Herein, we identify noncanonical chitinase-like enzyme TgCLP1 localizes micronemes is targeted after secretion. Genetic deletion resulted thickened decreased turnover, blocked export virulence effectors into host...
ABSTRACT Cellular adaptations to change often involve post-translational modifications of nuclear and cytoplasmic proteins. An example found in protists plants is the modification serine threonine residues dozens hundreds nucleocytoplasmic proteins with a single fucose (O-fucose). A O-fucosyltransferase occurs pathogen Toxoplasma gondii , social amoeba Dictyostelium higher plants, where it called Spy because mutants have spindly appearance. O-fucosylation, which required for optimal...
Duplication of the single Golgi apparatus in protozoan parasite Trypanosoma brucei has been followed by tagging a putative enzyme and matrix protein with variants GFP. Video microscopy shows that new appears de novo, near to old Golgi, about two hours into cell cycle grows over two-hour period until it is same size as Golgi. endoplasmic reticulum (ER) export site follows exactly time course. Photobleaching experiments show not exclusive product ER site. Rather, supplied, at least part,...
Determining the correct enzymatic activity of putative glycosyltransferases (GTs) can be challenging as these enzymes utilize multiple donor and acceptor substrates. Upon initial determination donor-sugar nucleotide(s), a GT utilizes various molecules that then tested. Here, we describe quick method to screen sugar-nucleotide specificities GTs utilizing sensitive, nonradioactive, commercially available bioluminescent uridine diphosphate detection kit. This in vitro allowed us validate...
Calmodulin purified from Dictyostelium discoideum is selectively degraded by rabbit reticulocyte extracts in the presence of ubiquitin and ATP. This protein forms a 1:1 covalent conjugate with ubiquitin. Analyses cyanogen bromide fragments indicate that lysine 115 on calmodulin conjugation site. Bovine brain which contains trimethyllysine residue at this position not substrate for ubiquitin, its degradation rate affected ATP These results suggest mammalian may function protecting ATP,...
Multiple lines of evidence suggest that Bordetella species have a significant life stage outside the mammalian respiratory tract has yet to be defined. The virulence gene (BvgAS) two-component system, paradigm for global regulon, controls expression many "virulence factors" expressed in Bvg positive (Bvg+) phase are necessary successful infection. A similarly large set highly conserved genes under negative (Bvg-) growth conditions; however, these appear primarily host and thus hypothesized...
Toxoplasma gondii is a protist parasite of warm-blooded animals that causes disease by proliferating intracellularly in muscle and the central nervous system. Previous studies showed prolyl 4-hydroxylase related to animal HIFα hydroxylases required for optimal proliferation, especially at low O2. We also observed Pro-154 Skp1, subunit Skp1/Cullin-1/F-box protein (SCF)-class E3-ubiquitin ligases, natural substrate this enzyme. In an unrelated protist, Dictyostelium discoideum, Skp1...
SKP1 is involved in the ubiquitination of certain cell cycle and nutritional regulatory proteins for rapid turnover. from Dictyostelium has been known to be modified by an oligosaccharide containing Fuc Gal, which unusual a cytoplasmic or nuclear protein. To establish how it glycosylated, labeled with [3H]Fuc was purified homogeneity digested endo-Lys-C. A single radioactive peptide found after two-dimensional high performance liquid chromatography. Analysis quadrupole time-of-flight mass...
Development in multicellular organisms is subject to both environmental and internal signals. In Dictyostelium, starvation induces amoebae form migratory slugs that translocate from subterranean areas exposed sites, where they culminate sessile fruiting bodies. Culmination, thought be regulated by anterior tip cells, selectively suppressed mild hypoxia a mechanism can partially overridden another signal, overhead light, or genetic activation of protein kinase A. Dictyostelium expresses, all...
Two techniques have been modified to provide simple means for the identification of molecules which bind concanavalin A (Con A). Crossed immunoelectrophoresis was altered by replacing antibody with Con A, and receptors were identified precipitin arcs they produced. tagged fluorescein isothiocyanate, also diffused into prefixed sodium dodecyl sulfate (SDS)-polyacrylamide gels, additional fluorescence. More than 35 in plasma membranes cellular slime mold Dictyostelium discoideum these...
We hypothesized that the peptidoglycan component of B. anthracis may play a critical role in morbidity and mortality associated with inhalation anthrax. To explore this issue, we purified bacterial cell wall studied response human peripheral blood cells. The was free non-covalently bound protein but contained complex set amino acids probably arising from stem peptide. polysaccharide removed by mild acid treatment, biological activity remained not polysaccharide. sensitive to lysozyme other...
The cell surface glycoprotein γ-glutamyl transpeptidase (GGT) was isolated from healthy human kidney and liver to characterize its glycosylation in normal tissue vivo. GGT is expressed by a single type the kidney. spectrum of N-glycans released constituted subset identified renal membrane glycoproteins. Recent advances mass spectrometry enabled us identify microheterogeneity relative abundance glycans on specific glycopeptides revealed broader than observed among enzymatically GGT. A total...
During advanced stages of inhalation anthrax, Bacillus anthracis accumulates at high levels in the bloodstream infected host. This bacteremia leads to sepsis during late-stage anthrax; however, mechanisms through which B. anthracis-derived factors contribute pathology hosts are poorly defined. Peptidoglycan, a major component cell wall Gram-positive bacteria, can provoke symptoms animal models. We have previously shown that peptidoglycan induce production proinflammatory cytokines by cells...
Specific protein glycoforms may be uniquely informative about the pathological state of a cyst and serve as accurate biomarkers. Here we tested that hypothesis using antibody-lectin sandwich arrays in broad screens targeted studies candidate markers. We profiled 16 different proteins captured by 72 antibodies fluid from mucinous nonmucinous cysts (n = 22), then three-marker panel 22 addition samples blinded samples. Glycan alterations were not widespread among mainly confined to MUC5AC...