A5042140942- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cancer-related gene regulation
- Immune cells in cancer
- vaccines and immunoinformatics approaches
- Head and Neck Cancer Studies
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Ferroptosis and cancer prognosis
- Pancreatic and Hepatic Oncology Research
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- CAR-T cell therapy research
- Traditional and Medicinal Uses of Annonaceae
- Genomics and Chromatin Dynamics
- Acute Lymphoblastic Leukemia research
Instituto Nacional do Câncer
2017-2025
National Cancer Institute
2025
Universidade Estadual de Campinas (UNICAMP)
2021-2023
Centro de Excelência em Bioinformática
2022
Weatherford College
2021
Institute of Bioinformatics
2021
Universidade Federal de Minas Gerais
2013-2018
Fundação Oswaldo Cruz
2018
Center for Integrated Protein Science Munich
2015-2016
Ludwig-Maximilians-Universität München
2015-2016
A novel EGFR-tyrosine kinase inhibitor (TKI), osimertinib, has marked efficacy in patients with EGFR-mutated lung cancer. However, some show intrinsic resistance and an insufficient response to osimertinib. This study showed that osimertinib stimulated AXL by inhibiting a negative feedback loop. Activated was associated EGFR HER3 maintaining cell survival inducing the emergence of cells tolerant inhibition reduced viability cancer overexpressing were exposed The addition during either...
Abstract To decrypt the regulatory code of genome, sequence elements must be defined that determine kinetics RNA metabolism and thus gene expression. Here, we attempt such decryption in an eukaryotic model organism, fission yeast S. pombe . We first derive improved genome annotation redefines borders 36% expressed mRNA s adds 487 non‐coding (nc s). then combine labeling vivo with mathematical modeling to obtain rates synthesis degradation for 5,484 splicing 4,958 introns. identify functional...
Abstract Tumor-associated myeloid-derived cells (MDCs) significantly impact cancer prognosis and treatment responses due to their remarkable plasticity tumorigenic behaviors. Here, we integrate single-cell RNA-sequencing data from different types, identifying 29 MDC subpopulations within the tumor microenvironment. Our analysis reveals abnormally expanded across various tumors distinguishes cell states that have often been grouped together, such as TREM2+ FOLR2+ subpopulations. Using...
Annexin A2 (ANXA2) is upregulated in several malignancies, including colorectal cancer (CRC). However, there little knowledge on the molecular mechanisms involved to its upregulation. The aim of this study was identify mechanism through which ANXA2 overexpression leads CRC progression and evaluate potential prognostic value. We used human samples analyse correlation between levels tumour staging. expression increased tissues compared normal colon tissues. In addition, we observe stage IV...
Dietary restriction (DR) reduces adiposity and improves metabolism in patients with one or more symptoms of metabolic syndrome. Nonetheless, it remains elusive whether the benefits DR humans are mediated by calorie nutrient restriction. This study was conducted to determine isocaloric dietary protein is sufficient confer beneficial effects We performed a prospective, randomized controlled intervention under constant nutritional medical supervision. Twenty-one individuals diagnosed syndrome...
Abstract Background DNA methylation (DNAm) age constitutes a powerful tool to assess the molecular and overall health status of biological samples. Recently, it has been shown that tissue-specific DNAm predictors may present superior performance compared pan- or multi-tissue counterparts. The skin is largest organ in body bears important roles, such as temperature control, barrier function, protection from external insults. As consequence constant intimate interaction between environment,...
Abstract Cellular senescence is known to play a role in age-related skin function deterioration which potentially influences longevity. Here, two-step phenotypic screening was performed identify senotherapeutic peptides, leading the identification of Peptide (Pep) 14. Pep 14 effectively decreased human dermal fibroblast burden induced by Hutchinson-Gilford Progeria Syndrome (HGPS), chronological aging, ultraviolet-B radiation (UVB), and etoposide treatment, without inducing significant...
Abstract Background Approximately 3/4 of ovarian cancers are diagnosed in advanced stages, with the high‐grade epithelial carcinoma (EOC) accounting for 90% cases. EOC present high genomic instability and somatic loss‐of‐function variants genes associated homologous recombination mutational repair pathway (HR), such as BRCA1 BRCA2 , TP53 . The identification germline HR is relevant treatment platinum resistant tumors relapsed therapies based synthetic lethality PARP inhibitors. Patients may...
The world is dealing with one of the worst pandemics ever. SARS-CoV-2 etiological agent COVID-19 that has already spread to more than 200 countries. However, infectivity, severity, and mortality rates do not affect all countries equally. Here we consider 140 HLA alleles extensively investigate landscape 3,723 potential HLA-I A B restricted SARS-CoV-2-derived antigens how 37 in are predicted respond those peptides considering their distribution frequencies. clustering HLA-A HLA-B allele...
Abstract Background Interaction between malignant cells and immune that reside within the tumor microenvironment (TME) modulate different aspects of development progression. Recent works showed importance miRNA-containing extracellular vesicles in this crosstalk. Methods Interested understanding interplay melanoma immune-related TME cells, we characterized TCGA’s metastatic samples according to their profiles, HLA-I neoepitopes, transcriptome profile classified them into three groups....
THE SPLICED LEADER (SL) IS A GENE THAT GENERATES FUNCTIONAL NCRNA COMPOSED OF TWO REGIONS: an intronic region of unknown function (SLi) and exonic (SLe), which is transferred to the 5' end independent transcripts yielding mature mRNAs, in a process known as spliced leader trans-splicing (SLTS). The best described for SLTS solve polycistronic into monocistronic units, specifically Trypanosomatids. In other metazoans, it speculated that SLe addition could lead increased mRNA stability,...
Abstract Background The aging of the skin, particularly around periorbital region, is a complex process characterized by accumulation senescent cells, decreased collagen production, and reduced skin elasticity, leading to visible signs such as fine lines, wrinkles, sagging. Objective This study investigates efficacy novel topical formulation, OS‐01 EYE, containing senomorphic peptide, OS‐01, along with other active ingredients, in improving eyes. Methods A 12‐week clinical was conducted 22...
BRAF inhibitor-resistant melanomas (BRAFiR) acquire (epi)genetic and functional alterations that enable them to evade alternative treatments. Identifying these is critical advancing treatment strategies. Here, we explored the effect of Chk1 inhibition (Chk1i) on BRAFiR cells, revealing higher sensitivity compared treatment-naive cells both in vitro vivo. Using FUCCI-labeling time-lapse microscopy, show S phase progression required for Chk1i-induced cytotoxicity but not cells. Replication...
SIN3 is a critical component of the histone deacetylase complex. Utilizing whole transcriptome data from melanoma patient samples we reveal that elevated levels SIN3B are associated with poor survival outcomes in vitro studies showing increased expression BRAF-mutant metastatic cell lines. The generation isogenic knockout lines indicated disruption led to decrease pathways tumor invasion, migration, and cell-cell interactions. Moreover, pooled genome-wide CRISPR/Cas9 screens highlighted...
The human papillomavirus (HPV) is present in a significant fraction of head-and-neck squamous cell cancer (HNSCC). main goal this study was to identify distinct co-expression patterns between HPV+ and HPV- HNSCC provide insights into potential regulatory mechanisms/effects within the analyzed networks. We selected cases deposited Cancer Genome Atlas database comprising data gene expression, methylation profiles mutational patterns, addition clinical information. intersection among...
Invariant natural killer T (iNKT) cells are a distinct population of lymphocytes characterized by their reactivity to glycolipids presented CD1d. iNKT found throughout the body, and little is known about tissue-specific metabolic regulation. Here, we show that splenic hepatic metabolically comparable rely on glycolytic metabolism support activation. Deletion pyruvate kinase M2 (Pkm2) gene in impairs response specific stimulation ability mitigate acute liver injury. In contrast, adipose...
Introduction: Several cell populations from the peripheral immune system interact to create a complex immunologic status during glioblastoma growth and response therapy. The aim of this study was integrate impact different present in tumor microenvironment on overall survival. Methodology: Gene expression clinical data were generated by Cancer Genome Atlas previously reported meta-signatures representing cells used. relationship between evaluated through Pearson's correlation analyses....
Esophageal squamous cell carcinoma (ESCC) presents poor prognosis, and patients diagnosed with this tumor currently lack target treatments. Therefore, in order to identify potential targets for ESCC treatment, we carried out a transcriptome analysis paired nonmalignant surrounding mucosa samples, followed by master regulator analysis, further explored the role of identified central regulatory genes through vivo vitro assays. Among transcription factors deregulated/enriched ESCC, focused on...
Gastric carcinoma (GC) is one of the most aggressive cancers and second leading cause cancer death in world. According to Lauren classification, this adenocarcinoma divided into two subtypes, intestinal diffuse, which differ their clinical, epidemiological molecular features. Several studies have attempted delineate signature gastric develop new non-invasive screening tests that improve diagnosis lead treatment strategies. However, a consensus has not yet been identified for each condition....
To identify underlying mechanisms involved with metastasis formation in Wilms tumors (WTs), we performed comprehensive DNA methylation and gene expression analyses of matched normal kidney (NK), WT blastemal component, metastatic tissues (MT) from patients treated under SIOP 2001 protocol. A linear Bayesian framework model identified 497 differentially methylated positions (DMPs) between groups that discriminated NK WT, but MT samples were divided two groups. Accordingly, variance grouped...