A5078024039- Pain Mechanisms and Treatments
- Pharmacological Effects of Natural Compounds
- Immune Response and Inflammation
- Skin Protection and Aging
- Neuropeptides and Animal Physiology
- Research on Leishmaniasis Studies
- IL-33, ST2, and ILC Pathways
- Natural product bioactivities and synthesis
- Stress Responses and Cortisol
- Bee Products Chemical Analysis
- Phytochemicals and Antioxidant Activities
- Inflammasome and immune disorders
- Tryptophan and brain disorders
- Exercise and Physiological Responses
- Ion Channels and Receptors
- Fatty Acid Research and Health
- Nitric Oxide and Endothelin Effects
- Trypanosoma species research and implications
- Genomics, phytochemicals, and oxidative stress
- Dermatology and Skin Diseases
- Inflammatory mediators and NSAID effects
- Essential Oils and Antimicrobial Activity
- melanin and skin pigmentation
- Bioactive Compounds and Antitumor Agents
- Fibromyalgia and Chronic Fatigue Syndrome Research
Universidade Estadual de Londrina
2016-2025
Universidad de la República
2017
Fundação Carlos Chagas
2017
Fundação Oswaldo Cruz
2017
Universidade de São Paulo
2004-2015
Universidade Federal de Minas Gerais
2015
Universidade de Ribeirão Preto
2004-2015
Universidade Estadual de Maringá
2012
Clinics Hospital of Ribeirão Preto
2006-2008
Zero to Three
2007
The hypernociceptive effects of cytokines [TNF-alpha, keratinocyte-derived chemokine (KC), and IL-1beta] their participation in carrageenan (Cg)-induced inflammatory hypernociception mice were investigated. Nociceptor sensitization (hypernociception) was quantified with an electronic version the von Frey filament test WT TNF receptor type 1 knockout (TNF-R1-/-). TNF-alpha-induced abolished TNF-R1-/- mice, partially inhibited by pretreatment IL-1 antagonist (IL-1ra) or indomethacin unaffected...
The aim of the present investigation was to describe and validate an electronic mechanical test for quantification intensity inflammatory nociception in mice. pressure-meter consists inducing animal hindpaw flexion reflex by poking plantar region with a polypropylene pipette tip adapted hand-held force transducer. This method compared classical von Frey filaments which pressure is automatically recorded after nociceptive reflex. were used detect time versus treatment interactions...
Abstract Neutrophil migration is responsible for tissue damage observed in inflammatory diseases. Neutrophils are also implicated nociception, but mechanisms of their participation have not been elucidated. In the present study, we addressed these carrageenan-induced mechanical hypernociception, which was determined using a modification Randall-Sellito test rats. accumulation into plantar by contents myeloperoxidase activity, whereas cytokines and PGE2 levels were measured ELISA...
The flavonoid vitexin (1) is a flavone C-glycoside (apigenin-8-C-β-d-glucopyranoside) present in several medicinal and other plants. Plant extracts containing 1 are reported to possess antinociceptive, anti-inflammatory, antioxidant activities. However, the only evidence that exhibits antinociceptive activity was demonstrated acetic acid-induced writhing model. Therefore, analgesic effects mechanisms of were evaluated. In investigation, intraperitoneal treatment with dose-dependently...
Vanillic acid (1) is a flavoring agent found in edible plants and fruits. It an oxidized form of vanillin. Phenolic compounds substantial part plant foods used as antioxidants with beneficial biological activities. These have received considerable attention because their role preventing human diseases. Especially, 1 presents antibacterial, antimicrobial, chemopreventive effects. However, the mechanisms by which exerts its anti-inflammatory effects vivo are incompletely understood. Thus,...
Although cellular senescence may be a protective mechanism in modulating proliferative capacity, fibroblast is now recognized as key pathogenic idiopathic pulmonary fibrosis (IPF). In aged mice, abundance and persistence of apoptosis-resistant senescent fibroblasts play central role nonresolving lung after bleomycin challenge. Therefore, we investigated whether quercetin can restore the susceptibility IPF to proapoptotic stimuli mitigate bleomycin-induced mice. Unlike normal fibroblasts,...
<h3>Objectives</h3> Interleukin 33 (IL-33) is a new member of the IL-1 family cytokines which signals via its receptor, ST2 (IL-33R), and has an important role in Th2 mast cell responses. This study shows that IL-33 orchestrates neutrophil migration arthritis. <h3>Methods results</h3> Methylated bovine serum albumin (mBSA) challenge knee joint mBSA-immunised mice induced local accompanied by increased IL-33R mRNA expression. Cell was inhibited systemic treatments with soluble (s)IL-33R,...
The objective of the present investigation was to compare sensitivity an electronic nociceptive mechanical paw test with classical tests quantify intensity variation inflammatory nociception. pressure-meter consists inducing hindpaw flexion reflex by poking plantar region a polypropylene pipette tip adapted hand-held force transducer. This method compared von Frey filaments and rat constant pressure test, modification Randall Selitto developed our group. When comparing three methods, but not...
Morphine is one of the most prescribed and effective drugs used for treatment acute chronic pain conditions. In addition to its central effects, morphine can also produce peripheral analgesia. However, mechanisms underlying this action have not yet been fully elucidated. Here, we show that antinociceptive effect lost in neuronal nitric-oxide synthase null mice induces production nitric oxide primary nociceptive neurons. The activation pathway by was dependent on an initial stimulation...
IL-17 is an important cytokine in the physiopathology of rheumatoid arthritis (RA). However, its participation genesis nociception during RA remains undetermined. In this study, we evaluated role articular a model antigen (mBSA)-induced arthritis. We found that mBSA challenge femur-tibial joint immunized mice induced dose- and time-dependent mechanical hypernociception. The local concentration within mBSA-injected joints increased significantly over time. Moreover, co-treatment challenged...
IL-33, a new member of the IL-1 family, signals through its receptor ST2 and induces T helper 2 (Th2) cytokine synthesis mediates inflammatory response. We have investigated role IL-33 in antigen-induced hypernociception. Recombinant induced cutaneous articular mechanical hypernociception time- dose-dependent manner. The was inhibited by soluble (s) (a decoy IL-33), antagonist (IL-1ra), bosentan [a dual endothelin (ET)(A)/ET(B) antagonist], clazosentan (an ET(A) antagonist), or indomethacin...
Quercetin (1) is an anti-inflammatory and antioxidant flavonoid. However, the oral administration of 1 did not lead to beneficial effects in experimental animal colitis models, which involve cytokines oxidative stress. A possible explanation that absorption profile prevents its activity. Therefore, it was reasoned controlled release would improve therapeutic effect. Thus, effect mechanisms 1-loaded microcapsules acetic acid-induced mice were evaluated. Microcapsules prepared using...
Background and purpose: Chemokines orchestrate neutrophil recruitment to inflammatory foci. In the present study, we evaluated participation of three chemokines, KC/CXCL1, MIP‐2/CXCL2 LIX/CXCL5, which are ligands for chemokine receptor 2 (CXCR2), in mediating immune inflammation induced by antigen immunized mice. Experimental approach: Neutrophil was assessed mice challenged with methylated bovine serum albumin, LIX/CXCL5 or tumour necrosis factor (TNF)‐α. Cytokine levels were determined...
Quercetin (1) is known to have both antioxidant and antinociceptive effects. However, the mechanism involved in its effect not fully elucidated. Cytokines reactive oxygen species been implicated cascade of events resulting inflammatory pain. Therefore, we evaluated 1 focusing on role cytokines oxidative stress. Intraperitoneal oral treatments with dose-dependently inhibited nociception induced by acetic acid phenyl-p-benzoquinone also second phase formalin- carrageenin-induced mechanical...
Ultraviolet B (UVB) irradiation may cause inflammation- and oxidative-stress-dependent skin cancer premature aging. Naringenin (1) has been reported to have anti-inflammatory antioxidant properties, but its effects mechanisms on UVB irradiation-induced inflammation oxidative stress are still not known. Thus, the present study aimed investigate potential of naringenin mitigate damage in hairless mice. Skin edema, myeloperoxidase (neutrophil marker) matrix metalloproteinase-9 (MMP-9) activity,...