A5107226686- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Immune Response and Inflammation
- Organoselenium and organotellurium chemistry
- Dermatology and Skin Diseases
- Cytokine Signaling Pathways and Interactions
- Adenosine and Purinergic Signaling
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammatory mediators and NSAID effects
- Stress Responses and Cortisol
- NF-κB Signaling Pathways
- Pharmacological Effects of Natural Compounds
- Toxin Mechanisms and Immunotoxins
- Antimicrobial Peptides and Activities
- Autoimmune and Inflammatory Disorders Research
- Plant Toxicity and Pharmacological Properties
- Connective tissue disorders research
- Complement system in diseases
- Urticaria and Related Conditions
- IL-33, ST2, and ILC Pathways
- Chemokine receptors and signaling
- Systemic Lupus Erythematosus Research
- Ion Channels and Receptors
King's College London
2016-2024
Hospital de Santa Maria
2024
University of Lisbon
2024
Administração Regional de Saúde de Lisboa e Vale do Tejo
2024
Universidade Federal de Alagoas
2024
Universidade de São Paulo
2010-2019
Universidade de Ribeirão Preto
2019
Clinics Hospital of Ribeirão Preto
2016
Universidade Federal de Santa Maria
2006-2007
IL-17 is an important cytokine in the physiopathology of rheumatoid arthritis (RA). However, its participation genesis nociception during RA remains undetermined. In this study, we evaluated role articular a model antigen (mBSA)-induced arthritis. We found that mBSA challenge femur-tibial joint immunized mice induced dose- and time-dependent mechanical hypernociception. The local concentration within mBSA-injected joints increased significantly over time. Moreover, co-treatment challenged...
Neuropathic pain from injury to the peripheral and CNS represents a major health care issue. We have investigated role of IL-33/IL-33 receptor (ST2) signaling in experimental models neuropathic mice. Chronic constriction (CCI) sciatic nerve induced IL-33 production spinal cord. IL-33/citrine reporter mice revealed that oligodendrocytes are main cells expressing within cord together with minor expression by neurons, microglia. astrocytes. CCI-induced mechanical hyperalgesia was reduced IL-33R...
Rheumatoid arthritis (RA) is an inflammatory autoimmune disease characterized by joint destruction and severe morbidity. Methotrexate (MTX) the standard first-line therapy of RA. However, about 40% RA patients are unresponsive to MTX treatment. Regulatory T cells (Tregs, CD4(+)CD25(+)FoxP3(+)) thought play important role in attenuating To investigate Tregs resistance, we recruited 122 (53 responsive, R-MTX; 69 unresponsive, UR-MTX) 33 healthy controls. Three months after treatment, R-MTX but...
Objective Infiltration of neutrophils into the joints plays an important role in bone erosion and articular destruction rheumatoid arthritis (RA). Neutrophil trafficking during inflammation is a process that involves activation chemotactic receptors. Recent findings suggest changes receptor patterns could occur under certain inflammatory conditions. The aim this study was to evaluate gain responsiveness CCL2 RA patients assess driving neutrophil infiltration joints. Methods Neutrophils were...
IL-33 signals through ST2 receptors and induces adaptive innate inflammation. IL-33/ST2 is involved in inflammation-induced pain. Here, we have investigated the contribution of IL-33/ST2-triggered mechanisms to carrageenin-induced inflammation.Carrageenin- IL-33-induced inflammatory responses were assessed BALB/c- (WT) ST2-deficient ((-/-) ) mice as follows: oedema (plethysmometer), myeloperoxidase activity (colorimetric assay), mechanical hyperalgesia (electronic version von Frey...
Significance Persistent pain in inflammatory and neuropathic conditions is often refractory to conventional analgesic therapy, with most patients suffering unrelieved serious treatment-related side effects. There still a tremendous need identify novel therapeutics for control innovative biological mechanisms minimal In this paper we challenge the hypothesis that conserved structural motif across G protein-coupled receptor family plays regulatory role negative modulation of activation use...
Neutrophil migration to inflamed sites is crucial for both the initiation of inflammation and resolution infection, yet these cells are involved in perpetuation different chronic inflammatory diseases. Gastrin-releasing peptide (GRP) a neuropeptide that acts through G protein coupled receptors (GPCRs) signal transmission central peripheral nervous systems. Its receptor, gastrin-releasing receptor (GRPR), expressed by various cell types, it overexpressed cancer cells. RC-3095 selective GRPR...
Abstract Fructose 1,6-bisphosphate (FBP) is an endogenous intermediate of the glycolytic pathway. Exogenous administration FBP has been shown to exert protective effects in a variety ischemic injury models, which are attributed its ability sustain glycolysis and increase ATP production. Here, we demonstrated that single treatment with markedly attenuated arthritis, assessed by reduction articular hyperalgesia, joint swelling, neutrophil infiltration production inflammatory cytokines, TNF...
Abstract The assessment of articular nociception in experimental animals is a challenge because available methods are limited and subject to investigator influence. In an attempt solve this problem, the purpose study was establish use dynamic weight bearing (DWB) as new device for evaluating joint model antigen-induced arthritis (AIA) mice. AIA induced Balb/c C57BL/6 mice evaluated by DWB. Western Blotting real-time PCR were used determine protein mRNA expression, respectively. DWB detected...
Epidemiologic studies have highlighted the association of environmental factors with development and progression autoimmune chronic inflammatory diseases. Among factors, smoking has been associated increased susceptibility poor prognosis in rheumatoid arthritis (RA). However, immune molecular mechanism smoking-induced aggravation remains unclear. The transcription factor aryl hydrocarbon receptor (AHR) regulates generation Th17 cells, CD4 T cells linked AHR is activated by organic compounds...
Intracellular pattern recognition receptors such as the nucleotide-binding oligomerization domain (NOD)-like family members are key for innate immune of microbial infection and may play important roles in development inflammatory diseases, including rheumatic diseases. In this study, we evaluated role NOD1 NOD2 on experimental arthritis. Ag-induced arthritis was generated wild-type, NOD1(-/-), NOD2(-/-), or receptor-interacting serine-threonine kinase 2(-/-) (RIPK2(-/-)) immunized mice...
Background: Caspase-1 is a cysteine protease responsible for the processing and secretion of IL-1β IL-18, which are closely related to induction inflammation. However, limited evidence addresses participation caspase-1 in inflammatory pain. Here, we investigated role hypernociception (a decrease nociceptive threshold) using deficient mice (casp1−/−). Results: Mechanical was evaluated an electronic version von Frey test. The production cytokines, PGE 2 neutrophil migration were by ELISA,...
To evaluate the antiinflammatory effects of RC-3095 in 2 experimental models arthritis, collagen-induced arthritis (CIA) and antigen-induced (AIA), to determine mechanisms action involved.RC-3095 was administered daily mice with CIA AIA, after induction disease methylated bovine serum albumin. Disease incidence severity were assessed using a clinical index evaluation histologic features, respectively. In CIA, gastrin-releasing peptide receptor (GRPR) detected by immunohistochemical analysis,...
Objectives: The aim of this study was to analyse the expression and function nucleotide-binding oligomerization domain (NOD)1 NOD2 in isolated cells patients with rheumatoid arthritis (RA).Method: mRNA levels NOD1, NOD2, receptor-interacting serine/threonine kinase 2 (RIPK2) genes were determined by quantitative polymerase chain reaction (qPCR) peripheral blood mononuclear (PBMCs) synovial fluid T (SFTCs) from RA osteoarthritis (OA) patients. Cytokines measured enzyme-linked immunosorbent...
The cholinergic anti-inflammatory pathway (CAP) is a complex neuroimmune mechanism triggered by the central nervous system to regulate peripheral inflammatory responses. Understanding role of CAP in pathogenesis rheumatoid arthritis (RA) could help develop new therapeutic strategies for this disease. Therefore, we investigated participation on progression experimental arthritis. Using antigen-induced (AIA) model, mice effects vagotomy or pharmacological treatments with hexamethonium...
The prostaglandin, 15-deoxy<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">Δ</mml:mi></mml:mrow><mml:mrow><mml:mn mathvariant="normal">12,14</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math>-prostaglandin J 2 (15d-PGJ ), is a lipid mediator that plays an important role in the control of chronic inflammatory disease. However, prostanoid rheumatoid arthritis (RA) not well determined. We demonstrated therapeutic...
Prolonged exposure to opioids causes an enhanced sensitivity painful stimuli (opioid-induced hyperalgesia, OIH) and a need for increased opioid doses maintain analgesia tolerance, OIT), but the mechanisms underlying both processes remain obscure. We found that pharmacological block or genetic deletion of HCN2 ion channels in primary nociceptive neurons male mice completely abolished OIH had no effect on OIT. Conversely, inhibition central HCN alleviated OIT OIH. Expression C-FOS, marker...
Endothelin mediates neutrophil recruitment during innate inflammation. Herein we address whether endothelin-1 (ET-1) is involved in adaptive inflammation mice, and its mechanisms. Pharmacological treatments were used to determine the role of endothelin peritoneal cavity mice challenged with antigen (ovalbumin) or ET-1. Levels ET-1, tumour necrosis factor α (TNFα), CXC chemokine ligand 1 (CXCL1) determined by enzyme-linked immunosorbent assay. Neutrophil migration flow cytometry analyses...
Paclitaxel (PCX) is the first-line choice for treatment of several types cancer, including breast, ovarian, and lung cancers. However, patients who receive even a single dose with PCX commonly develop mechanical cold allodynia, symptom known as PCX-associated acute pain syndrome (P-APS). Here, we assessed possible involvement kinin-kallikrein renin-angiotensin systems in P-APS mice.Male mice C57Bl/6 wild type (WT) knockouts bradykinin receptors, B1 (B1-/- ) B2 (B2-/- ), were used. Mechanical...