A5059375939- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Insect symbiosis and bacterial influences
- COVID-19 and healthcare impacts
- Autoimmune and Inflammatory Disorders Research
- HIV Research and Treatment
- Global Maternal and Child Health
- Hemoglobinopathies and Related Disorders
- Vector-borne infectious diseases
- COVID-19 and Mental Health
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Youth, Drugs, and Violence
- Sepsis Diagnosis and Treatment
- Lipid Membrane Structure and Behavior
- Maternal and Neonatal Healthcare
- Inflammasome and immune disorders
Fundação Oswaldo Cruz
2016-2025
University of Oxford
2021
Brigham and Women's Hospital
2014-2017
Harvard University
2017
Universidade de São Paulo
2008-2016
Universidade Federal de Pernambuco
2016
Universidade de Ribeirão Preto
2010-2014
Attack rate in Manaus Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) incidence peaked Manaus, Brazil, May 2020 with a devastating toll on the city's inhabitants, leaving its health services shattered and cemeteries overwhelmed. Buss et al. collected data from blood donors São Paulo, noted when transmission began to fall, estimated final attack rates October (see Perspective by Sridhar Gurdasani). Heterogeneities immune protection, population structure, poverty, modes of public...
To provide an initial description of the congenital syndrome presumably associated with infection by Zika virus compared other syndromes including infections established etiologies.We overview a published case series 35 cases, clinical 104 and unpublished reports laboratory findings describing cases diagnosed since beginning epidemic microcephaly in Brazil.About 60% to 70% mothers report rash during pregnancy; mainly first trimester. Principal features are microcephaly, facial...
The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus Flaviviridae family into major public health concern. Little is currently known about evolution and biology ZIKV factors that contribute to associated pathogenesis. Determining genomic sequences clinical viral isolates characterization elements within these are an important prerequisite advance our understanding replicative processes virus-host interactions.We obtained...
Zika virus (ZIKV) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. The primarily transmitted by mosquito bite, although routes infection have implicated in some cases. Aedes aegypti considered to be the main vector humans worldwide; however, there evidence species, including Culex quinquefasciatus, transmit virus. To test potential Cx. quinquefasciatus ZIKV, we experimentally compared competence...
Anti-Flavivirus antibodies are highly cross-reactive and may facilitate Zika virus (ZIKV) infection through the antibody-dependent enhancement (ADE) mechanism. We demonstrate that dengue-specific enhance of a primary Brazilian ZIKV isolate in FcγRII-expressing K562 cell line. In addition, we serum samples from dengue-immune pregnant women enhanced infection. These findings highlight need for epidemiological studies animal models to further confirm role ADE development congenital neurological...
Rheumatoid arthritis (RA) is an inflammatory autoimmune disease characterized by joint destruction and severe morbidity. Methotrexate (MTX) the standard first-line therapy of RA. However, about 40% RA patients are unresponsive to MTX treatment. Regulatory T cells (Tregs, CD4(+)CD25(+)FoxP3(+)) thought play important role in attenuating To investigate Tregs resistance, we recruited 122 (53 responsive, R-MTX; 69 unresponsive, UR-MTX) 33 healthy controls. Three months after treatment, R-MTX but...
Chikungunya virus (CHIKV) is a re-emerging arbovirus, in which infection causes febrile illness also commonly associated with severe joint pain and myalgia. Although the immune response to CHIKV has been studied, better understanding of virus-host interaction mechanisms may lead more effective therapeutic interventions. In this context, neutrophil extracellular traps (NETs) have described as key mediator involved control many pathogens, including several bacteria viruses, but no reports...
BackgroundSince 2015, the arthropod-borne viruses (arboviruses) Zika and chikungunya have spread across Americas causing outbreaks, accompanied by increases in immune-mediated infectious neurological disease. The spectrum of manifestations linked to these viruses, importance dual infection, are not known fully. We aimed investigate whether presentations differed according infecting arbovirus, patients with infection had a different disease or severity.MethodsWe report prospective...
Zika virus (ZIKV) infection during pregnancy is associated with microcephaly, a congenital malformation resulting from neuroinflammation and direct effects of replication on the developing central nervous system (CNS). However, exact changes in affected CNS remain unknown. Here, we show by transcriptome analysis (at 48 hours post-infection) multiplex immune profiling that human induced-neuroprogenitor stem cells (hiNPCs) respond to ZIKV strong induction type I interferons (IFNs) several IFNs...
After the Zika virus (ZIKV) epidemic in Americas 2016, both and dengue incidence declined to record lows many countries 2017-2018, but 2019 resurged Brazil, causing ~2.1 million cases. In this study we use epidemiological, climatological genomic data investigate dynamics recent years Brazil. First, estimate force of infection (FOI) model mosquito-borne transmission suitability since early 2000s. Our estimates reveal that DENV was low despite conditions being suitable for viral spread. also...
Brain infections pose substantial challenges in diagnosis and management carry high mortality morbidity, especially low-income middle-income countries. We aimed to improve the early of patients admitted hospital (adults aged 16 years older children >28 days) with suspected acute brain at 13 hospitals Brazil, India, Malawi. With stakeholders, policy makers, patient public representatives, we co-designed a multifaceted clinical laboratory intervention, informed by an evaluation routine...
The recent global outbreak of Zika virus (ZIKV) infection has been linked to severe neurological disorders affecting the peripheral and central nervous systems (PNS CNS, respectively). pathobiology underlying these diverse clinical phenotypes are subject intense research; however, even principal neural cell types vulnerable productive remain poorly characterised. Here we used CNS PNS myelinating cultures from wild type Ifnar1 knockout mice examine neuronal glial tropism short-term...
Zika virus (ZIKV) has been identified in several body fluids of infected individuals. In most cases, it remained detected blood from few days to 1 week after the onset symptoms, and can persist longer urine semen. ZIKV infection have dramatic consequences such as microcephaly Guillain-Barré syndrome. sexual transmission documented. A better understanding presence persistence across biologic compartments is needed devise rational measures prevent its transmission.This observational cohort...
Abstract The herd immunity threshold is the proportion of a population that must be immune to an infectious disease, either by natural infection or vaccination such that, in absence additional preventative measures, new cases decline and effective reproduction number falls below unity 1 . This fundamental epidemiological parameter still unknown for recently-emerged COVID-19, mathematical models have predicted very divergent results 2,3 Population studies using antibody testing infer total...
Abstract We generated 238 Zika virus (ZIKV) genomes from 135 persons in Brazil who had samples collected over 1 year to evaluate persistence. Phylogenetic inference clustered the together with previously reported ZIKV strains northern Brazil, showing that has been remained relatively stable time. Temporal phylogenetic analysis revealed limited within-host diversity among most ZIKV-persistent infected associated samples. However, we detected unusual temporal >5 persons, uncovering existence...
Intracellular pattern recognition receptors such as the nucleotide-binding oligomerization domain (NOD)-like family members are key for innate immune of microbial infection and may play important roles in development inflammatory diseases, including rheumatic diseases. In this study, we evaluated role NOD1 NOD2 on experimental arthritis. Ag-induced arthritis was generated wild-type, NOD1(-/-), NOD2(-/-), or receptor-interacting serine-threonine kinase 2(-/-) (RIPK2(-/-)) immunized mice...
B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using prediction tools. Mapping these onto the surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% between ZIKV four dengue (DENV) serotypes, were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for dramatically divergent electrostatic potential on regions DENV2 serotypes. These...
Defining cases of Zika virus (ZIKV) infection is a critical challenge for epidemiological research. Due to ZIKV’s overlapping clinical features and potential immunologic cross-reactivity with other flaviviruses the current lack an optimal ZIKV-specific diagnostic assay, varying approaches identifying ZIKV infections have been employed date. This paper presents laboratory results criteria developed by Microcephaly Epidemic Research Group defining maternal in cohort pregnant women rash (N =...
Viral encephalitis is a common cause of lethal infections in humans, and several different viruses are documented to be responsible. Rocio virus flavivirus that causes severe syndrome humans also mice, providing an interesting model study the CNS compartmentalized immune response. Interleukin 33 (IL-33), member IL-1 family, immunomodulatory cytokine highly expressed CNS. However, role IL-33 on viral remains unclear. Therefore, we aimed explore how IL-33/ST2 axis regulates local response...
ObjectiveTo determine the clinical phenotype of Guillain-Barré syndrome (GBS) after Zika virus (ZIKV) infection, anti-glycolipid antibody signature, and role other circulating arthropod-borne viruses, we describe a cohort GBS patients identified during ZIKV chikungunya (CHIKV) outbreaks in Northeast Brazil.MethodsWe prospectively recruited from regional neurology center Brazil between December 2014 February 2017. Serum CSF were tested for ZIKV, CHIKV, dengue (DENV), by RT-PCR antibodies,...