A5041910989- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- Hemoglobinopathies and Related Disorders
- Research on Leishmaniasis Studies
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 detection and testing
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- IL-33, ST2, and ILC Pathways
- Carbohydrate Chemistry and Synthesis
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- Click Chemistry and Applications
- COVID-19 Clinical Research Studies
- Synthesis and Biological Evaluation
- Immune Response and Inflammation
- Stress Responses and Cortisol
- Biochemical and Molecular Research
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Hepatitis B Virus Studies
- Extracellular vesicles in disease
Universidade Estadual de Campinas (UNICAMP)
2019-2024
Brazilian Biosciences National Laboratory
2021-2023
Brazilian Center for Research in Energy and Materials
2021-2023
Universidade de São Paulo
2010-2020
Washington University in St. Louis
2014-2020
Clinics Hospital of Ribeirão Preto
2012-2016
Universidade de Ribeirão Preto
2013-2015
Mutations accrued by SARS-CoV-2 lineage P.1-first detected in Brazil early January, 2021-include amino acid changes the receptor-binding domain of viral spike protein that also are reported other variants concern, including B.1.1.7 and B.1.351. We aimed to investigate whether isolates wild-type P.1 can escape from neutralising antibodies generated a polyclonal immune response.We did an immunological study assess effects on B SARS-CoV-2, using plasma samples patients previously infected with...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak tract disease known as Coronavirus Disease 2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such lymphocytopenia cytokine storm, which are associated with severity predict mortality. mechanism by infection result in immune system dysfunction still not fully understood. Here, we show that human CD4+ T helper cells, but CD8+ present blood...
Trypanosoma cruzi trans-sialidase (TcTS) plays a key role in the recognition and invasion of host cells enabling parasite to escape human immune response. To explore this potential drug target, we have synthesized small library substrate analogues based on 1,4-disubstituted 1,2,3-triazole derivatives galactose modified at either C-1 or C-6 positions. This was achieved by coupling appropriate azido-sugars with panel 23 structurally diverse terminal alkynes using copper(I)-catalyzed...
Hyperbaric oxygen (HBO) therapy is a well-established method for improving tissue oxygenation and typically used the treatment of various inflammatory conditions, including infectious diseases. However, its effect on intestinal mucosa, microenvironment known to be physiologically hypoxic, remains unclear. Here, we demonstrated that daily with hyperbaric affects gut microbiome composition, worsening antibiotic-induced dysbiosis. Accordingly, HBO-treated mice were more susceptible...
Abstract The innate immune response to Trypanosoma cruzi infection comprises several pattern recognition receptors (PRRs), including TLR-2, -4, -7, and -9, as well the cytosolic receptor Nod1. However, there are additional PRRs that account for host responses T. cruzi. In this context, nucleotide-binding oligomerization domain–like (NLRs) activate inflammasomes candidate deserve renewed investigation. Following pathogen infection, NLRs form large molecular platforms, termed inflammasomes,...
A multi-step cascade strategy using integrated ligand- and target-based virtual screening methods was developed to select a small number of compounds from the ZINC database be evaluated for trypanocidal activity. Winnowing 23 selected compounds, 12 non-covalent binding cruzain inhibitors with affinity values (K i) in low micromolar range (3-60 µM) acting through competitive inhibition mechanism were identified. This has been confirmed by determining mode inhibitor Nequimed176 X-ray...
ABSTRACT Oropouche virus (OROV) is a member of the Orthobunyavirus genus in Bunyaviridae family and prominent cause insect-transmitted viral disease Central South America. Despite its clinical relevance, little known about OROV pathogenesis. To define host defense pathways that control infection disease, we evaluated pathogenesis immune responses primary cells mice were deficient RIG-I-like receptor signaling pathway (MDA5, RIG-I, or MAVS), downstream regulatory transcription factors (IRF-3...
Background: A new SARS-CoV-2 lineage, named P.1 (20J/501Y.V3), has recently been detected in Brazil. Mutations accrued by the lineage include amino acid changes receptor-binding domain of spike protein that also are reported variants concern United Kingdom (B.1.1.7) and South Africa (B.1.325).Methods: We isolated two P.1-containing specimens from nasopharyngeal bronchoalveolar lavage samples patients Manaus, measured neutralization virus after incubation with plasma 19 COVID-19 convalescent...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak tract disease known as disease-2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such lymphocytopenia cytokine storm, which are associated with severity predict mortality 1,2 . mechanism by infection result in immune system dysfunction still not fully understood. Here we show that human CD4 + T helper cells, but CD8 present blood...
This work describes the synthesis of a series sialylmimetic neoglycoconjugates represented by 1,4-disubstituted 1,2,3-triazole-sialic acid derivatives containing galactose modified at either C-1 or C-6 positions, glucose gulose C-3 position, and amino derivative 1,2,3-triazole fused threonine-3-O-galactose as potential TcTS inhibitors anti-trypanosomal agents. was obtained Cu(I)-catalysed azide-alkyne cycloaddition reaction ('click chemistry') between azido-functionalized sugars 1-N(3)-Gal...
Here, we investigated the ability of IFN-γ to modulate functions mouse neutrophils in vitro. Neutrophils incubated presence showed enhanced phagocytosis response zymosan, opsonized zymosan or precipitated immune complexes IgG and ovalbumin. The effect was dose-dependent with an initial at 10 U/ml a maximal 150 U/ml; 2 h incubation were required reach optimal level. These stimuli can also induce IFN-γ-pretreated release reactive oxygen species (ROS), such as superoxide anion, hydrogen...
ABSTRACT Interferon (IFN)-regulatory factor 5 (IRF-5) is a transcription that induces inflammatory responses after engagement and signaling by pattern recognition receptors. To define the role of IRF-5 during bunyavirus infection, we evaluated Oropouche virus (OROV) La Crosse (LACV) pathogenesis immune in primary cells mice with gene deletions Irf3 , Irf5 Irf7 or alone. Deletion together resulted uncontrolled viral replication liver spleen, hypercytokinemia, extensive injury, an early-death...
The parasite Trypanosoma cruzi causes Chagas disease, which remains a serious public health concern and continues to victimize thousands of people, primarily in the poorest regions Latin America. In search for new therapeutic drugs against T. cruzi, here we have evaluated both vitro vivo activity 5-hydroxy-3-methyl-5-phenyl-pyrazoline-1-(S-benzyl dithiocarbazate) (H2bdtc) as free compound or encapsulated into solid lipid nanoparticles (SLN); compared results with those achieved by using...
Coxsackievirus B (CVB) is a common cause of acute and chronic infectious myocarditis pancreatitis. Th1 cells producing IFN-γ TNF-α are important for CVB clearance, but they also associated with the pathogenesis inflammatory lesions, suggesting that modulation Th2 balance likely in controlling CVB-induced We investigated role IL-33, which an recently discovered cytokine induction Th2-associated responses, experimental CVB5 infection. found mice deficient IL-33R, T1/ST2, significantly...
Viral encephalitis is a common cause of lethal infections in humans, and several different viruses are documented to be responsible. Rocio virus flavivirus that causes severe syndrome humans also mice, providing an interesting model study the CNS compartmentalized immune response. Interleukin 33 (IL-33), member IL-1 family, immunomodulatory cytokine highly expressed CNS. However, role IL-33 on viral remains unclear. Therefore, we aimed explore how IL-33/ST2 axis regulates local response...
Oropouche virus (OROV) is a frequent cause of arboviral febrile disease in the Amazon. The present report describes studies done two patients, one them; first OROV human case acquired outside Amazon, which have revealed for time presence peripheral blood leukocytes. This novel finding raises important issues regarding pathogenesis infections and may offer new tool, rapid diagnosis this neglected infection. J. Med. Virol. 89:1108–1111, 2017 . © Wiley Periodicals, Inc.
Background Chagas disease remains a serious medical and social problem in Latin America is an emerging concern nonendemic countries as result of population movement, transfusion infected blood or organs congenital transmission. The current treatment patients unsatisfactory due to strain-specific drug resistance the side effects medications. For this reason, discovery safer more effective chemotherapy mandatory for successful future eradication disease. Methods Findings We investigated effect...
Background Chronic Chagas cardiomyopathy in humans is characterized by segmental left ventricular wall motion abnormalities ( WMA ), mainly the early stages of disease. This study aimed at investigating detection and its correlation with underlying histopathological changes a chronic model hamsters. Methods Results Female Syrian hamsters (n=34) infected 3.5×10 4 or 10 5 blood trypomastigote Trypanosoma cruzi (Y strain) forms an uninfected control group (n=7) were investigated. After 6 months...