A5025494289- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Parasites and Host Interactions
- Malaria Research and Control
- Synthesis and Biological Evaluation
- Parasitic Infections and Diagnostics
- Helminth infection and control
- Vector-borne infectious diseases
- Whipple's Disease and Interleukins
- Parasitic Diseases Research and Treatment
- Extracellular vesicles in disease
- Insect Pest Control Strategies
- Virology and Viral Diseases
- Immune cells in cancer
- Insect and Pesticide Research
- Reproductive System and Pregnancy
- Click Chemistry and Applications
- Liver Disease Diagnosis and Treatment
- Insect symbiosis and bacterial influences
- Microbial Community Ecology and Physiology
- Lysosomal Storage Disorders Research
- Adenosine and Purinergic Signaling
Universidade Federal do Rio Grande do Norte
2016-2025
Universidade de São Paulo
2008-2014
Clinics Hospital of Ribeirão Preto
2010-2012
Universidade de Ribeirão Preto
2010
Universidade Federal de Ouro Preto
2002-2009
Universidade Federal de Minas Gerais
2008
Fundação Oswaldo Cruz
1998
Universidade Federal do Triângulo Mineiro
1998
Universidade Federal do Rio de Janeiro
1998
Secretaria Municipal de Saúde
1998
Background Chagas disease is a neglected caused by the intracellular parasite Trypanosoma cruzi. Around 30% of infected patients develop chronic cardiomyopathy or megasyndromes, which are high-cost morbid conditions. Immune response against myocardial self-antigens and exacerbated Th1 cytokine production has been associated with pathogenesis disease. As IL-17 involved in several autoimmune, inflammatory infectious diseases, we investigated its role during infection T. Methodology/Principal...
Trypanosoma cruzi trans-sialidase (TcTS) plays a key role in the recognition and invasion of host cells enabling parasite to escape human immune response. To explore this potential drug target, we have synthesized small library substrate analogues based on 1,4-disubstituted 1,2,3-triazole derivatives galactose modified at either C-1 or C-6 positions. This was achieved by coupling appropriate azido-sugars with panel 23 structurally diverse terminal alkynes using copper(I)-catalyzed...
Aim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups twenty-one each, given following treatments for 10 days: (1) no ligature + water, (2) (3) 50 mg/kg Met, (4) 100 (5) 200 Met. Water or Met was administered orally. Maxillae fixed scanned using Micro-computed Tomography (μCT) to quantitate linear volume/tissue volume...
Background Myocardium damage during Chagas' disease results from the immunological imbalance between pro- and production of anti-inflammatory cytokines has been explained based on Th1–Th2 dichotomy regulatory T cell activity. Recently, we demonstrated that IL-17 produced experimental T. cruzi infection regulates Th1 cells differentiation parasite induced myocarditis. Here, investigated role human disease. Methodology/Principal Findings First, observed CD4+IL-17+ in culture peripheral blood...
Abstract An effective innate immune recognition of the intracellular protozoan parasite Trypanosoma cruzi is critical for host resistance against Chagas disease, a severe and chronic illness that affects millions people in Latin America. In this study, we evaluated participation nucleotide-binding oligomerization domain (Nod)-like receptor proteins response to T. infection found Nod1-dependent, but not Nod2-dependent, responses are required infection. Bone marrow-derived macrophages from...
ABSTRACT In this work, we investigated the in vivo activity of ravuconazole against Y and Berenice-78 Trypanosoma cruzi strains using acutely infected dogs as hosts. Ravuconazole was well tolerated, no significant side effects were observed during treatment 6.0 mg/kg twice a day (12 mg/kg/day) for up to 90 days. all treated animals, parasitemia permanently suppressed by first treatment, independently parasite strain. Cultures blood obtained posttreatment negative 90% confirming that drug...
Abstract The innate immune response to Trypanosoma cruzi infection comprises several pattern recognition receptors (PRRs), including TLR-2, -4, -7, and -9, as well the cytosolic receptor Nod1. However, there are additional PRRs that account for host responses T. cruzi. In this context, nucleotide-binding oligomerization domain–like (NLRs) activate inflammasomes candidate deserve renewed investigation. Following pathogen infection, NLRs form large molecular platforms, termed inflammasomes,...
Aim To evaluate the anti-inflammatory, anti-oxidant and antifibrotic effects of carvedilol (CARV) in rats with ethanol-induced liver injury. Methods Liver injury was induced by gavage administration alcohol (7 g/kg) for 28 consecutive days. Eighty Wistar were pretreated oral CARV at 1, 3, or 5 mg/kg saline 1 h before exposure to alcohol. homogenates assayed interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α level as well myeloperoxidase (MPO) activity malonyldialdehyde (MDA)...
Colorectal cancer is still unmanageable despite advances in target therapy. However, extracellular vesicles (EVs) have shown potential nanomedicine as drug delivery systems, especially for modulating the immune cells tumor microenvironment (TME). In this study, M1 Macrophage EVs (M1EVs) were used nanocarriers of oxaliplatin (M1EV1) associated with retinoic acid (M1EV2) and Libidibia ferrea (M1EV3), alone or combination (M1EV4) to evaluate their antiproliferative immunomodulatory on CT-26...
The discovery of the pharmacological functions nitric oxide has led to development NO donor compounds as therapeutic agents. A new generation ruthenium donors, cis-[Ru(NO)(bpy)(2)L]X(n), been developed, and our aim was show that these complexes are able lyse Trypanosoma cruzi in vitro vivo.NO donors were incubated with T. their anti-T. activities evaluated percentage lysed parasites compared negative control. In vivo, trypanocidal activity by observing levels parasitaemia, survival rate...
Background The protozoan Trypanosoma cruzi is the causative agent of Chagas disease. There are no vaccines or effective treatment, especially in chronic phase when most patients diagnosed. a clear necessity to develop new drugs and strategies for control treatment Recent papers have suggested ecto-nucleotidases (from CD39 family) from pathogenic agents as important virulence factors. In this study we evaluated influence Ecto-Nucleoside-Triphosphate-Diphosphohydrolase (Ecto-NTPDase) activity...
Albaconazole is an experimental triazole derivative with potent and broad-spectrum antifungal activity a remarkably long half-life in dogs, monkeys, humans. In the present work, we investigated vivo of this compound against two strains protozoan parasite Trypanosoma (Schizotrypanum) cruzi, causative agent Chagas' disease, using dogs as hosts. The T. cruzi used study were previously characterized (murine model) susceptible (strain Berenice-78) partially resistant Y) to drugs currently...
ABSTRACT Trypanosoma cruzi infection causes intense myocarditis, leading to cardiomyopathy and severe cardiac dysfunction. Protective adaptive immunity depends on balanced signaling through a T cell receptor coreceptors expressed the surface. Such can trigger stimulatory or inhibitory signals after binding their ligands in antigen-presenting cells (APC). T. modulates expression of lymphocytes infection. Deregulated inflammation may be due unbalanced these molecules. Programmed death 1 (PD-1)...
Caulerpin (CLP), an alkaloid from algae of the genus Caulerpa, has shown anti-inflammatory activity. Therefore, this study aimed to analyze effect CLP in murine model peritonitis and ulcerative colitis. Firstly, mice were submitted evaluate which dose (40, 4, or 0.4 mg/kg) could decrease inflammatory infiltration peritoneum. The most effective doses 40 4 mg/kg. Then, C57BL/6 colitis development with 3% dextran sulfate sodium (DSS) treated at disease was analyzed through activity index (DAI);...
Objective The aim of this study was to evaluate the effects gliclazide on oxidative stress, inflammation, and bone loss in an experimental periodontal disease model. Material Methods Male albino Wistar rats were divided into no ligature, ligature with 1, 5, 10 mg/kg groups. Maxillae fixed scanned using micro-computed tomography quantify linear volume/tissue volume (BV/TV) volumetric loss. Histopathological, immunohistochemical immunofluorescence analyses conducted examine matrix...
One of the main reasons for cancer's low clinical response to chemotherapeutics is highly immunosuppressive tumor microenvironment (TME). Tumor-ass ociated M2 macrophages (M2-TAMs) orchestrate immunosuppression, which favors progression. Extracellular vesicles (EVs) have shown great potential targeted therapies as, depending on their biological origin, they can present different therapeutic properties, such as enhanced accumulation in target tissue or modulation immune system. In current...
Determining esophageal and colon involvement in patients with Chagas disease occurs through invasive uncomfortable examinations, which most cases are not performed. The objective of this study was to assess the anti-M2-pyruvate kinase (M2-PK) autoantibodies development digestive alterations and/or diagnosis form human disease. total IgG isotype (IgG1, IgG2, IgG3, IgG4) production quantified using antigen Trypanosoma cruzi M2-PK recombinant protein via ELISA technique. tests were conducted...
Dogs are the main domestic reservoir host of parasite Trypanosoma cruzi, causative agent Chagas disease, and they considered sentinel animals for detection new cases human infection. Canis familiaris is also a well-established experimental T. cruzi infection model, but little known about progression disease in naturally-infected dogs, especially during acute phase these hosts. Triatomine species infected with discrete typing units (DTUs) I, II III have been previously found many...