A5007040642- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Cytomegalovirus and herpesvirus research
- Rabies epidemiology and control
- Research on Leishmaniasis Studies
- Macrophage Migration Inhibitory Factor
- Trypanosoma species research and implications
- Viral Infections and Vectors
- Venomous Animal Envenomation and Studies
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Heme Oxygenase-1 and Carbon Monoxide
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Vector-borne infectious diseases
- Cardiovascular Issues in Pregnancy
- Immune Cell Function and Interaction
- Coccidia and coccidiosis research
- Healthcare and Venom Research
- Bacteriophages and microbial interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Impact on Reproduction
- Neuropeptides and Animal Physiology
- Antimicrobial Peptides and Activities
Universidade Federal de Uberlândia
2016-2025
AC Camargo Hospital
2024
University of California, Los Angeles
2022-2023
Universidade de Ribeirão Preto
2013
Universidade de São Paulo
2009-2013
Universidade Estadual Paulista (Unesp)
2004-2011
Universidade Brasil
2009
Núcleo de Pesquisas Aplicadas (Brazil)
2004
ABSTRACT Considering that little is known about the epidemiology of Neospora caninum infection in humans, particularly populations with high Toxoplasma gondii rates, present study aimed to investigate presence antibodies N. T. -seropositive and -seronegative individuals. A total 256 serum samples divided into four groups (61 from human immunodeficiency virus [HIV]-positive patients, 50 patients neurological disorders, 91 newborns, 54 healthy subjects) were assessed for serologies by indirect...
Abstract An effective innate immune recognition of the intracellular protozoan parasite Trypanosoma cruzi is critical for host resistance against Chagas disease, a severe and chronic illness that affects millions people in Latin America. In this study, we evaluated participation nucleotide-binding oligomerization domain (Nod)-like receptor proteins response to T. infection found Nod1-dependent, but not Nod2-dependent, responses are required infection. Bone marrow-derived macrophages from...
Abstract Protozoan parasites of the genus Leishmania are causative agents Leishmaniasis, a disease that can be lethal and affects 12 million people worldwide. replicates intracellularly in macrophages, process is essential for progression. Although production reactive oxygen species (ROS) accounts restriction parasite replication, known to induce ROS upon macrophage infection. We have recently demonstrated NLRP3 inflammasome activation infected important outcome However, molecular mechanisms...
Toxoplasma gondii is an intracellular parasite that causes severe disease when the infection occurs during pregnancy. Trophoblast cells constitute important maternal-fetal barrier, with monocytes concentrating around them. Thus, interactions between trophoblasts and are for maintaining a successful pregnancy, especially in cases of infection. This study aimed to evaluate role trophoblast (BeWo line) on monocyte (THP-1 activity presence or absence T. infection.THP-1 were stimulated...
Depressive disorder during pregnancy is a common condition, affecting approximately 10–15% of pregnant women, and associated with adverse outcomes such as inadequate prenatal care, substance abuse, fetal growth restriction. Beyond neurotransmitter disturbances, increasing evidence suggests that infectious agents may play role in the pathophysiology depression through immune system modulation. Toxoplasma gondii infection has been linked to various mental disorders general population,...
Toxoplasmosis and neosporosis have been recognized as economically important diseases with considerable impact on the livestock industry. Considering scarce information occurrence of Toxoplasma gondii Neospora caninum infections in sheep from Uberlândia, Minas Gerais State, Brazil, this study aimed to investigate frequency antibodies against these parasites sera region by using different serological methods. A total 155 serum samples were analyzed indirect fluorescence antibody test (IFAT)...
Neospora caninum is an apicomplexan parasite responsible for major economic losses due to abortions in cattle. Toll‐like receptors (TLRs) sense specific microbial products and direct downstream signaling pathways immune cells, linking innate, adaptive immunity. Here, we analyze the role of TLR2 on innate responses during N. infection. Inflammatory peritoneal macrophages bone marrow‐derived dendritic cells exposed ‐soluble antigens presented upregulated expression TLR2. Increased receptor was...
Background Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used different approaches, including diagnosis the study host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as necessity animal bleeding, low yield, interference with rheumatoid factor, complement activation affinity to Fc receptors. IgY avoid those limitations; therefore they...
Neospora caninum is a protozoan parasite that presents worldwide distribution and mainly implicated as responsible for bovine abortion. Although the presence of birds in cattle-raising properties positively correlated to higher infection rates, very little has been described about role these animals parasite's life cycle. In sense, this work aimed investigate serological histological positivity different avian species sampled its natural habitat or captivity. No was observed 294 tested serum...
The combination of sulfadiazine and pyrimethamine plus folinic acid is the conventional treatment for congenital toxoplasmosis. However, this classical presents teratogenic effects bone marrow suppression. In sense, new therapeutic strategies are necessary to reduce these improve control infection. context, biogenic silver nanoparticles (AgNp-Bio) appear as a promising alternative since they have antimicrobial, antiviral, antiparasitic activity. purpose study investigate action AgNp-Bio in...
SUMMARY Neospora caninum causes economical impact in cattle-raising farms since it is implicated as the major cause of bovine abortions. Although infection by parasite has been widely described mammals, role birds its life-cycle still obscure. Therefore, this work aimed to evaluate N. different chicken models. Experimental infections were conducted 7-day-old chicks, laying hens and embryonated eggs, where samples analysed for burden, IgG antibodies lesions promoted. Chickens demonstrated an...
CpG oligodeoxynucleotides (ODN) have shown to be potent immunoadjuvants for several pathogens, but there is limited information concerning their use in immunization protocols against neosporosis. This study aimed evaluate the potential of CpG-ODN combined with Neospora lysate antigen (NLA) or excreted-secreted (NcESA) induce protective immune response caninum infection mice. C57BL/6 mice were vaccinated subcutaneously three times at 2-week intervals NLA, NLA + CpG, NcESA, NcESA (adjuvant...
Neospora caninum is an intracellular parasite that causes major economic impact on cattle raising farms, and infects a wide range of warm-blooded hosts worldwide.Innate immune mechanisms lead to protection against this are still unknown.In order investigate whether myeloid differentiation factor 88 (MyD88) required for resistance N. caninum, genetically deficient mice (MyD88 À/À ) wild type littermates were infected with live tachyzoites the infection was evaluated.We found sub-lethal...
ArtinM and Jacalin (JAC) are lectins from the jackfruit (Artocarpus integrifolia) that have important role in modulation of immune responses to pathogens. Neospora caninum is an Apicomplexa parasite causes neuromuscular disease dogs reproductive disorders cattle, with economic impact on livestock industry. Hence, we evaluated adjuvant effect JAC immunization mice against neosporosis. Six C57BL/6 mouse groups were subcutaneously immunized three times at 2-week intervals lysate antigen (NLA)...
Considering that the current immunoassays are not able to distinguish infective forms cause Toxoplasma gondii infection, present study was carried out evaluate reactivity of two recombinant proteins (CCp5A and OWP1) from oocyst/sporozoite, in order differentiate infections occurring by ingestion oocysts or tissue cysts. The assessed against panels serum samples animals (chickens, pigs mice) were naturally experimentally infected different stages parasite. Also, we tested sera humans who have...
Due to the high prevalence and economic impact of neosporosis, development safe effective vaccines therapies against this parasite has been a priority in field is crucial limit horizontal vertical transmission natural hosts. Limited data available regarding factors that regulate immune response such knowledge essential order understand Neospora caninum induced pathogenesis. Mitogen-activated protein kinases (MAPKs) govern diverse cellular processes, including growth, differentiation,...