A5056776784- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Blood Pressure and Hypertension Studies
- Ion channel regulation and function
- Electron Spin Resonance Studies
- Ion Transport and Channel Regulation
- Cardiovascular Health and Disease Prevention
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Protease and Inhibitor Mechanisms
- Sodium Intake and Health
- Caveolin-1 and cellular processes
- Renal and Vascular Pathologies
- Cardiac Ischemia and Reperfusion
- Lanthanide and Transition Metal Complexes
- Magnetism in coordination complexes
- Cardiac electrophysiology and arrhythmias
- Cerebrovascular and Carotid Artery Diseases
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Aortic Disease and Treatment Approaches
Universidade de São Paulo
2015-2024
Universidade de Ribeirão Preto
2014-2023
SUPERA Park of Innovation and Technology of Ribeirão Preto
2004-2017
JP Group (Brazil)
2012
De La Salle-College of Saint Benilde
2011
Clinics Hospital of Ribeirão Preto
2004-2010
University of California, Santa Barbara
2007
Rede de Química e Tecnologia
2006
Universidade Brasil
2003
University of Michigan–Ann Arbor
1995
Mounting evidence indicates that structural and functional vascular changes associated with two-kidney, one-clip (2K-1C) hypertension result, at least in part, from altered activity of matrix metalloproteinases (MMPs). Because MMPs are upregulated by increased formation reactive oxygen species (ROS), we hypothesized antioxidant approaches could attenuate the increases MMP-2 expression/activity dysfunction remodeling 2K-1C hypertension. Sham-operated or hypertensive rats were treated tempol...
Background Myocardium damage during Chagas' disease results from the immunological imbalance between pro- and production of anti-inflammatory cytokines has been explained based on Th1–Th2 dichotomy regulatory T cell activity. Recently, we demonstrated that IL-17 produced experimental T. cruzi infection regulates Th1 cells differentiation parasite induced myocarditis. Here, investigated role human disease. Methodology/Principal Findings First, observed CD4+IL-17+ in culture peripheral blood...
Endothelial dysfunction is the hallmark of hypertension, which a multifactorial disorder. In cardiovascular system reactive oxygen species play pivotal role in controlling endothelial function and vascular tone. Physiologically, endothelium-derived relaxing factors (EDRFs) contractile (EDCFs) that have functions on smooth muscle cells. The relaxation induced by EDRFs nitric oxide (NO), prostacyclin, hyperpolarization factor (EDHF) could be impaired hypertension. ability cells to release NO...
Vascular dysfunction associated with two-kidney, one-clip (2K-1C) hypertension may result from both altered matrix metalloproteinase (MMP) activity and higher concentrations of reactive oxygen species (ROS). Doxycycline is considering the most potent MMP inhibitor tetracyclines attenuates 2K-1C hypertension-induced high blood pressure chronic vascular remodeling. might also act as a ROS scavenger this contribute to amelioration some cardiovascular diseases increased ROS. We hypothesized that...
Nitric oxide (NO) plays an important role in the control of vascular tone and most often employed NO donors have limitations due to their harmful side-effects. In this context, new been prepared, order minimize such undesirable effects. cis-[Ru(bpy)2(py)NO2](PF6) (RuBPY) is a nitrite complex synthesized our laboratory that releases presence tissue only. work vasorelaxation induced by donor has studied compared obtained with well known SNP. The relaxation RuBPY concentration-dependent denuded...
We investigated the involvement of nitric oxide (NO) and reactive oxygen species (ROS) on autonomic cardiovascular parameters, vascular reactivity, endothelial cells isolated from aorta monosodium glutamate (MSG) obese rats. Obesity was induced by administration 4 mg/g body wt MSG or equimolar saline [control (CTR)] to newborn At 60th day, treatment started with N(G)-nitro-L-arginine methyl ester (L-NAME, 20 mg/kg) 0.9% saline. 90th after artery catheterization, mean arterial pressure (MAP)...
The hypothesis that signal transduction mediated by platelet-derived growth factor (PDGF) and angiotensin II (Ang II) is altered in vascular smooth muscle (VSM) cells from the spontaneously hypertensive rat (SHR) was tested measuring changes cytosolic free calcium concentration ([Ca2+]i). [Ca2+]i measured cultured aortic SHRs Wistar-Kyoto (WKY) normotensive rats using fura-2 as a indicator microscopic digital image analysis system. Activation of with Ang resulted prompt though transient rise...
Drugs that release nitric oxide (NO) usually have limitations due to their harmful effects. Sodium nitroprusside (SNP) induces a rapid hypotension leads reflex tachycardia, which could be an undesirable effect in patients with heart disease, common feature of hypertension. The nitrosyl ruthenium complex [Ru(terpy)(bdq)NO+]3+ (TERPY) is NO donor less potent than SNP denuded aortic rings. This study evaluated the and vasorelaxation induced by this Wistar (W) spontaneously hypertensive rats...
Background and purpose: Benznidazole (Bz) is the therapy currently available for clinical treatment of Chagas' disease. However, many strains Trypanosoma cruzi parasites are naturally resistant. Nitric oxide (NO) produced by activated macrophages crucial to intracellular killing parasites. Here, we investigate in vitro vivo activities against T. , NO donor, trans‐[RuCl([15]aneN 4 )NO] 2+ . Experimental approach: Trans‐ [RuCl([15]aneN was incubated with a partially drug‐resistant Y strain...
We investigated the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The response to HCl-induced (7.4 6.5) was measured aortic rings pre-contracted with phenylephrine (Phe, 10− 6 M) or KCl (45 mM). vascular reactivity experiments were performed endothelium-intact and denuded rings, presence absence of indomethacin (10− 5 M), L-NAME 4 apamin glibenclamide M). effect acidosis (pH 7.0 on nitric oxide (NO) production evaluated isolated endothelial cells...
Aims It has been known for more than a century that pH changes can alter vascular tone. However, there is no consensus about the effects of on response. In this study, we investigated extracellular (pHo) intracellular (pHi) and nitric oxide concentration ([NO]i) in freshly isolated endothelial cells cross sections from rat aorta. Main Methods The HCl was used to reduce pHo 7.4 7.0 6.5; NaOH increase 8.0 8.5. fluorescent dyes 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate (SNARF-1)...