Karina Bispo-dos-Santos
A5025209262- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Olfactory and Sensory Function Studies
- Vaccine Coverage and Hesitancy
- Respiratory viral infections research
- COVID-19 and Mental Health
- Extracellular vesicles in disease
- Infection Control and Ventilation
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- T-cell and Retrovirus Studies
- Education during COVID-19 pandemic
- Immune cells in cancer
- Influenza Virus Research Studies
- COVID-19 Impact on Reproduction
- Vector-Borne Animal Diseases
- Gut microbiota and health
- Insect Pest Control Strategies
Universidade Estadual de Campinas (UNICAMP)
2020-2025
Universidade Federal de Roraima
2021
University of Oxford
2021
Universidade de São Paulo
2021
Imperial College London
2021
Universidade Feevale
2021
Mutations accrued by SARS-CoV-2 lineage P.1-first detected in Brazil early January, 2021-include amino acid changes the receptor-binding domain of viral spike protein that also are reported other variants concern, including B.1.1.7 and B.1.351. We aimed to investigate whether isolates wild-type P.1 can escape from neutralising antibodies generated a polyclonal immune response.We did an immunological study assess effects on B SARS-CoV-2, using plasma samples patients previously infected with...
Microbiota-derived molecules called short-chain fatty acids (SCFAs) play a key role in the maintenance of intestinal barrier and regulation immune response during infectious conditions. Recent reports indicate that SARS-CoV-2 infection changes microbiota SCFAs production. However, relevance this effect is unknown. In study, we used human biopsies epithelial cells to investigate impact by SARS-CoV-2. did not change entry or replication cells. These metabolites had no on cells' permeability...
Background: A new SARS-CoV-2 lineage, named P.1 (20J/501Y.V3), has recently been detected in Brazil. Mutations accrued by the lineage include amino acid changes receptor-binding domain of spike protein that also are reported variants concern United Kingdom (B.1.1.7) and South Africa (B.1.325).Methods: We isolated two P.1-containing specimens from nasopharyngeal bronchoalveolar lavage samples patients Manaus, measured neutralization virus after incubation with plasma 19 COVID-19 convalescent...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak tract disease known as disease-2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such lymphocytopenia cytokine storm, which are associated with severity predict mortality 1,2 . mechanism by infection result in immune system dysfunction still not fully understood. Here we show that human CD4 + T helper cells, but CD8 present blood...
Background/Objectives: Rice bran oil (RBO) is rich in phytochemical compounds and has many pharmaceutical applications. This work evaluated the regenerative potential of nanofibers incorporating RBO, focusing on their efficacy tissue engineering dermatological formulations. The main objective was to investigate impact RBO SK-MEL-28 melanoma cell migration wound closure through an vitro healing assay. In addition, biocompatibility adhesion properties were examined. Methods: study employed...
We documented 4 cases of severe acute respiratory syndrome coronavirus 2 reinfection by non-variant concern strains among healthcare workers in Campinas, Brazil. isolated infectious particles from nasopharyngeal secretions during both infection episodes. Improved and continued protection measures are necessary to mitigate the risk for workers.
Oropouche orthobunyavirus (OROV) is an emerging arbovirus with a high potential of dissemination in America. Little known about the role peripheral blood mononuclear cells (PBMC) response during OROV infection humans. Thus, to evaluate human leukocytes susceptibility, permissiveness and immune infection, we applied RNA hybridization, qRT-PCR cell-based assays quantify viral antigens, genome, antigenome gene expression different cells. First, observed replication lineages as THP-1 monocytes,...
Until December 2021, the COVID-19 pandemic has caused more than 5.5 million deaths. Vaccines are being deployed worldwide to mitigate severe disease and death, but continued transmission emergence of SARS-CoV-2 variants indicate that specific treatments against still necessary. We screened 400 compounds from Medicines for Malaria Venture (MMV) Pathogen Box seeking molecules with antiviral activity by using a high-throughput screening (HTS) infection assay in Vero CCL81 cells. On resupply 15...
Interest in clean beauty is rising due to minimalism the formulation of cosmetics, rational use water, and fewer chemical additives like preservatives, colorants, surfactants, artificial fragrances. Green ingredients lead development sustainable formulations with advanced performance are less aggressive human health environment. Currently, electrospinning technique used as a simple one-step manufacturing process produce nanostructured cosmetics under mild temperature conditions. This study...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is mainly transmitted by airborne droplets generated infected individuals. Since this and many other pathogens are able to remain viable on inert surfaces for extended periods of time, contaminated play an important role in SARS-CoV-2 fomite transmission. Cosmetic products destined be applied infection-sensitive sites, such as the lips eyelids. Therefore, special biosafety precautions should incorporated into routine procedures...
SARS-CoV-2 is an emerging virus from the Coronaviridae family and responsible for ongoing COVID-19 pandemic. In this work, we explored previously reported structural membrane protein (M) interaction with human Proliferating Cell Nuclear Antigen (PCNA). The M maintaining virion shape, PCNA a marker of DNA damage which essential replication repair. We validated M-PCNA through immunoprecipitation, immunofluorescence co-localization, PLA (Proximity Ligation Assay). cells infected or transfected...
A SARS-CoV-2 B.1.1.7 variant of concern (VOC) has been associated with increased transmissibility, hospitalization, and mortality. This study aimed to explore the factors VOC infection in context vaccination. On March 2021, we detected RNA nasopharyngeal samples from 14 22 individuals vaccinated a single-dose ChAdOx1 (outbreak A, n = 26), 42 two doses CoronaVac vaccine B, 52) for breakthrough rates 63.6% 52.4% CoronaVac. The outbreaks were caused by independent clusters VOC. serum...
This is the third year of SARS-CoV-2 pandemic, and yet most children remain unvaccinated. COVID-19 in manifests as mostly mild or asymptomatic, however high viral titers strong cellular humoral responses are observed upon acute infection. It still unclear how long these persist, if they can protect from re-infection and/or disease severity. Here, we analyzed immune memory a cohort adults with COVID-19. Important differences between evident kinetics profile responses. Children develop early...
The ongoing COVID-19 pandemic caused a significant loss of human lives and worldwide decline in quality life. Treatment patients is challenging, specific treatments to reduce aggravation mortality are still necessary. Here, we describe the discovery novel class epiandrosterone steroidal compounds with cationic amphiphilic properties that present antiviral activity against SARS-CoV-2 low micromolar range. Compounds were identified screening campaigns using cytopathic effect-based assay Vero...
The COVID-19 pandemic caused by the SARS-CoV-2 virus has highlighted need for serological assays that can accurately evaluate neutralizing efficiency of antibodies produced during infection or induced vaccines. However, conventional often require manipulation live viruses on a level-three biosafety (BSL3) facility, which presents practical and safety challenges. Here, we present novel, alternative assay measures (NAbs) against in plasma using flow cytometry. This is based antibody binding to...
BACKGROUND Zika virus (ZIKV) is an emerging arbovirus associated with foetal malformations and neurological complications. The infection usually mild symptoms. comparison between the allelic frequency of polymorphic genes in symptomatic infected individuals population can clarify pathogenic mechanisms ZIKV. During ZIKV infection, cytokines are produced natural killer (NK) cells recruited, whose activation depends on signaling pathways activated by specific receptors, such as cell...
Background: Some studies have determined that the SARS-CoV-2 B.1.1.7 variant of concern (VOC) has been associated with increased transmissibility, hospitalization, and mortality. This study aimed to explore factors VOC infection in context vaccination adenovirus-vectored inactivated vaccines.Methods: We analyzed epidemiological, clinical, laboratory data from simultaneous COVID-19 outbreaks a convent (Outbreak A) long-term care facility B) individuals vaccinated single dose ChAdOx1 or two...
Abstract The COVID-19 disease caued by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has two characteristics that distinguish it from other viral infections. It affects more severely people with pre-existing comorbidities and load peaks prior to onset of symptoms. Investigating factors could contribute these characteristics, we found increased mTOR signaling suppressed genes related autophagy, lysosome, vesicle fusion in Vero E6 cells infected SARS-CoV-2. Transcriptomic...