A5037703326- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Liver Disease Diagnosis and Treatment
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- HIV/AIDS drug development and treatment
- Research on Leishmaniasis Studies
- Blood donation and transfusion practices
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Synthesis and Biological Evaluation
- Syphilis Diagnosis and Treatment
- Sex work and related issues
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- HIV, Drug Use, Sexual Risk
- Virus-based gene therapy research
- Eosinophilic Disorders and Syndromes
- Parvovirus B19 Infection Studies
- Chronic Lymphocytic Leukemia Research
- Plant Virus Research Studies
Fundação Pró-Sangue Hemocentro de São Paulo
2015-2024
Universidade de São Paulo
1999-2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2018-2023
Universidade Federal de São Paulo
2013-2015
Attack rate in Manaus Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) incidence peaked Manaus, Brazil, May 2020 with a devastating toll on the city's inhabitants, leaving its health services shattered and cemeteries overwhelmed. Buss et al. collected data from blood donors São Paulo, noted when transmission began to fall, estimated final attack rates October (see Perspective by Sridhar Gurdasani). Heterogeneities immune protection, population structure, poverty, modes of public...
Background HIV-infected individuals have deficient responses to Yellow Fever vaccine (YFV) and may be at higher risk for adverse events (AE). Chronic immune activation–characterized by low CD4/CD8 ratio or high indoleamine 2,3-dioxygenase-1 (IDO) activity—may influence response in this population. Methods We prospectively assessed AE, viremia the YFV virus YF-specific neutralizing antibodies (NAb) (CD4>350) -uninfected adults through 1 year after vaccination. The effect of HIV status on...
Background Dengue virus transmission by blood transfusion is a rarely reported event. Case Report During dengue outbreak in S ão P aulo city, regular plateletpheresis donor informed the bank of being diagnosed few days after donation. The recipient was hospitalized and displayed symptoms laboratory evidence transfusion. Results immunoglobulin ( Ig ) G , IgM polymerase chain reaction nonreactive on index sample, seroconverting 20 later. platelet units were transfused into two patients. One...
Background: Many clinical studies have suggested a beneficial effect of GB virus type C (GBV-C) on the course HIV-1 infection, but mechanisms involved in such amelioration are not clear. As recent evidence has implicated cellular activation pathogenesis, we investigated GBV-C viremia T-cell early infection. Methods: Forty-eight recently infected patients (23 viremic) were evaluated for counts, expanded immunophenotyping RNA detection, and viral load. Nonparametric univariate multivariate...
Abstract The herd immunity threshold is the proportion of a population that must be immune to an infectious disease, either by natural infection or vaccination such that, in absence additional preventative measures, new cases decline and effective reproduction number falls below unity 1 . This fundamental epidemiological parameter still unknown for recently-emerged COVID-19, mathematical models have predicted very divergent results 2,3 Population studies using antibody testing infer total...
Background The results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency type 1 (HIV-1) due to similar transmission routes both viruses. aim this study was determine prevalence, incidence density and genotypic characteristics GBV-C population. Methodology/Principal Findings population included 233 from a cohort primarily comprised homosexual men recently HIV-1 São Paulo, Brazil. presence RNA...
Background Hepatitis C virus (HCV) infection is a global health problem estimated to affect almost 200 million people worldwide. The aim of this study analyze the subtypes and existence variants resistant protease inhibitors their association with potential HCV risk factors among blood donors in Brazil. Methods Repeat anti-HCV reactive are systematically asked return for retest, notification, counseling which they interviewed transfusion-transmitted diseases. We analyzed 202 who returned...
Abstract Background Chagas' disease ( CD ) is caused by infection with the protozoan T rypanosoma cruzi . The can affect heart and/or gastrointestinal GI tract, but around 70% of infected individuals remain asymptomatic in chronic form. Organ transplantation from ‐infected donors often avoided because risk transmission, previously reported after heart, kidney, or liver transplantation. Methods We investigated histology, immunohistochemistry, and polymerase chain reaction PCR persistence...
GB virus C (GBV-C) is an enveloped positive-sense ssRNA belonging to the Flaviviridae family. Studies on genetic variability of GBV-C reveals existence six genotypes: genotype 1 predominates in West Africa, 2 Europe and America, 3 Asia, 4 Southwest 5 South Africa 6 Indonesia. The aim this study was determine frequency genotypic distribution Colombian population. Two groups were analyzed: i) 408 blood donors infected with HCV (n = 250) HBV 158) from Bogotá ii) 99 indigenous people infection...
Entry inhibitor is a new class of drugs that target the viral envelope protein. This region variable; hence resistance to these may be present before treatment. The aim this study was analyze frequency patients failing treatment with transcriptase reverse and protease inhibitors would respond entry Enfuvirtide, Maraviroc, BMS-806. included 100 HIV-1 positive from one outpatient clinic in city Sao Paulo, for whom genotype test requested due failure. Proviral DNA amplified sequenced regions...
Previous studies have demonstrated that coinfection with HPgV is a protective factor for human immunodeficiency virus (HIV)-infected patients, leading to slower disease progression, and longer survival after established disease. The present study sought estimate the prevalence of infection associated risk factors in patients harboring C or non-C HIV-1 subtypes followed-up at HU-FURG, southern Brazil. Samples from 347 HIV-1-infected subjects were subjected plasma RNA extraction, cDNA...
Bacterial and aseptic meningitis after neurosurgery can present similar clinical signs symptoms. The aims of this study were to develop test a molecular method diagnose bacterial (BM) neurosurgery.A 16S ribosomal RNA gene PCR-based strategy was developed using artificially inoculated cerebrospinal fluid (CSF) followed by sequencing. tested CSF samples from 43 patients who had undergone suspected suffer meningitis, 8 without or meningitis. Patients classified into five groups, confirmed BM,...
We compared the cost-benefit of two algorithms, recently proposed by Centers for Disease Control and Prevention, USA, with conventional one, most appropriate diagnosis hepatitis C virus (HCV) infection in Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio ELISA anti-HCV that show s/co ³95%...
The objective of the present study was to evaluate serum viral load in chronically infected Hepatitis B virus (HBV) patients and investigate distribution HBV genotypes São Paulo city. Quantitative HBV-DNA assays genotyping have gained importance for predicting disease progression, been employed assessing infectivity, treatment monitoring detecting emergence drug resistance. Twenty-nine Brazilian with suspected chronic hepatitis were studied, using real time PCR determination direct DNA...
Background The participation of immune/inflammatory mechanisms in the pathogenesis tropical endomyocardial fibrosis (EMF) has been suggested by finding early blood and myocardial eosinophilia. However, inflammatory activation status late-stage EMF patients is still unknown. Methodology/Principal findings We evaluated pro- anti-inflammatory cytokine levels plasma samples from late stage patients. Cytokine Tumor Necrosis Factor (TNF)-α, Interferon (IFN)-γ, Interleukin (IL)-2, IL-4, IL-6, IL-10...
Abstract Background Chagas disease reactivation (CDR) after heart transplantation is characterized by relapse of the infectious with proliferation and dissemination Trypanosoma cruzi parasites. Serial blood PCR testing consensually recommended for CDR monitoring, but there uncertainty about incremental value in performing molecular tests endomyocardial biopsies (EMB). Methods We compared qualitative quantitative results T DNA 62 pairs EMB collected a maximum time interval 7 days, from 34...
The objective of this study was to characterize the prevalence viral encephalitis due arbovirus infection Togaviridae and Flaviviridae families in São Paulo, Brazil. A total 500 cerebrospinal fluid (CSF) samples collected between August 2012 January 2013, from patients with symptoms acute were analyzed. Findings suggestive encephalitis—elevations cell concentration, glucose protein—were observed 234 (46.8%) samples, designated as Group 1. remaining 266 comprised 2. All tested for...
It is recognized that hepatitis C virus subtypes (1a, 1b, 2a, 2b, 2c and 3a) originated in Africa Asia spread worldwide exponentially during the Second World War (1940) through transfusion of contaminated blood products, invasive medical dental procedures, intravenous drug use. The entry into different regions occurred at distinct times, presenting exponential growth rates larger or smaller spread. Our study estimated most prevalent currently circulating São Paulo. A total 465 non-structural...
Abstract Background The increasing incidence of syphilis worldwide has called attention to the risk transmission by transfusion. Aims To determine prevalence active in blood donors and characterise serological profile syphilis‐positive donors. Methods Samples positive for Treponema pallidum using chemiluminescent microparticle immunoassay (CMIA) during donor screening from 2017 2018 were tested Venereal Disease Research Laboratory (VDRL) non‐treponemal test anti‐ T. IgM ELISA (Immunoassay...
The present study determined the HBV antigen, antibody, and DNA status in blood donations deemed to be positive. Individuals with an occult infection (OBI), defined as being positive for but negative surface antigen (HBsAg), well those active (HBsAg-positive), were identified characterized.From a total pool if 198,363 donations, we evaluated cross-sectional study, 1106 samples that screening tests antibody core (HBcAb), HBsAg, and/or by nucleic acid testing (NAT-HBV). presence of genetic...