A5087933781- Air Quality and Health Impacts
- Energy and Environment Impacts
- Climate Change and Health Impacts
- Asthma and respiratory diseases
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- COVID-19 Clinical Research Studies
- Birth, Development, and Health
- Vascular Malformations and Hemangiomas
- Cannabis and Cannabinoid Research
- Adipose Tissue and Metabolism
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Breast Lesions and Carcinomas
- SARS-CoV-2 and COVID-19 Research
- Infection Control and Ventilation
- Healthcare during COVID-19 Pandemic
- Prenatal Substance Exposure Effects
- Pregnancy and preeclampsia studies
- Voice and Speech Disorders
- Long-Term Effects of COVID-19
- Breast Implant and Reconstruction
- Eosinophilic Disorders and Syndromes
- Environmental and Sediment Control
- Inhalation and Respiratory Drug Delivery
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2019-2024
Universidade de São Paulo
2014-2024
Universidade Estadual Paulista (Unesp)
2024
Universidade Federal do Sul da Bahia
2022
Universidade Federal do Paraná
2020
Centro Universitário das Faculdades Metropolitanas Unidas
2014-2017
Air pollution represents a considerable threat to health worldwide. The São Paulo Metropolitan area, in Brazil, has unique composition of atmospheric pollutants with population nearly 20 million people and 9 passenger cars. It is long known that exposure particulate matter less than 2.5 µm (PM2.5) can cause various effects such as DNA damage. One the most versatile defense mechanisms against accumulation damage nucleotide excision repair (NER), which includes XPC protein. However, by NER...
The prevalence of marijuana use among pregnant women is high. However, the effects on gestation and fetal development are not well known. Epidemiological experimental studies present conflicting results because route administration, dose, time exposure, species used, how Cannabis toxicity tested (prepared extracts, specific components, or by pyrolysis). In this study, we experimentally investigated maternal inhalation sativa smoke representing as nearly possible real world conditions human...
Acute respiratory distress syndrome (ARDS) has a high mortality rate of 35-46% depending on its severity. Animal models are crucial to better understand the pathophysiology diseases, including ARDS. This study presents feasible animal model acute lung injury (ALI) using nebulized lipopolysaccharide (LPS) in non-invasive approach, focusing short and long-term effects.Mice received LPS or vehicle only (control group). Blood, BALF tissue were collected 24 hours (LPS 24h) 5 weeks 5w) after...
Chitotriosidase (CHIT1) is an enzyme produced by macrophages that regulates their differentiation and polarization. Lung have been implicated in asthma development; therefore, we asked whether pharmacological inhibition of macrophage-specific CHIT1 would beneficial effects asthma, as it has shown previously other lung disorders. expression was evaluated the tissues deceased individuals with severe, uncontrolled, steroid-naïve asthma. OATD-01, a chitinase inhibitor, tested 7-week-long house...
BackgroundAirway remodeling is a prominent feature of asthma, which involves increased airway smooth muscle mass and altered extracellular matrix composition. Bronchial thermoplasty (BT), bronchoscopic treatment for severe targets remodeling.ObjectiveWe sought to investigate the effect BT on composition its association with clinical outcomes.MethodsThis substudy TASMA trial. Thirty patients asthma were BT-treated, whom 13 treated 6 months standard therapy (control group) before BT....
Abstract Background Lymphangioleiomyomatosis (LAM) is a rare neoplastic and cystic pulmonary disease characterized by abnormal proliferation of the so-called LAM cells. Despite functional obstructive pattern observed in most patients, few studies investigated morphological changes small airways, them patients with severe advanced undergoing lung transplantation. Understanding airways that may occur early can help us understand pathophysiology progression rationale for possible therapeutic...
Abstract Background Acute respiratory distress syndrome (ARDS) is a common cause of failure in critically ill patients, and diffuse alveolar damage (DAD) considered its histological hallmark. Sepsis one the most aetiology ARDS with highest case-fatality rate. Identifying patients differentiate them from other causes acute remains challenge. To address this, many studies have focused on identifying biomarkers that can help assess lung epithelial injury. However, there scarce information...
Abstract Background Lung fibrosis is a major concern in severe COVID-19 patients undergoing mechanical ventilation (MV). frequency post-COVID syndrome highly variable and even if the risk proportionally small, many could be affected. However, there still no data on lung extracellular matrix (ECM) composition whether it different from other aetiologies of ARDS. Methods We have quantified ECM elements TGF-β expression tissue 28 fatal cases compared to 27 that died causes ARDS, divided...
Abstract Evidence regarding the impact of air pollution on acute respiratory distress syndrome (ARDS) is limited, and most studies focus ARDS onset. Our study aimed to evaluate whether exposure fine particulate matter interferes with lung recovery remodeling in a murine model injury. Forty-eight mice received nebulized LPS or vehicle (controls). Blood, BALF, lungs spleen were collected after 5 weeks either PM 2.5 (PM + group) filtered (control LPS5w groups). Inflammatory cells cytokines...
Abstract Background The increasing incidence of syphilis worldwide has called attention to the risk transmission by transfusion. Aims To determine prevalence active in blood donors and characterise serological profile syphilis‐positive donors. Methods Samples positive for Treponema pallidum using chemiluminescent microparticle immunoassay (CMIA) during donor screening from 2017 2018 were tested Venereal Disease Research Laboratory (VDRL) non‐treponemal test anti‐ T. IgM ELISA (Immunoassay...
Abstract Objectives To evaluate the occurrence of systemic and renal abnormalities in offspring Wistar rats exposed to tenofovir disoproxil fumarate (DF) during pregnancy. Methods Female received a standard diet, with or without addition DF (100 mg/kg diet), 1 week before mating Offspring from group were placed an untreated foster mother breastfeeding compared maintained on diet pregnancy (control). Control followed up at 3 6 months age. Monthly body weight systolic blood pressure (SBP),...
Abstract Background Studies in adult severe treatment-resistant asthma (STRA) have demonstrated heterogeneous pathophysiology. the pediatric age group are still scarce, and few include bronchial tissue analysis. Objective We investigated 6–18-year-old patients diagnosed with STRA Sao Paulo, Brazil, by characterizing different lung compartments their correlations control function. Methods Inflammatory profiles of 13 a confirmed diagnosis were analyzed using blood, induced sputum,...
Tracheal replacement with a bioengineered tracheal substitute has been developed for long-segment diseases. The decellularized scaffold is an alternative cell seeding. It not defined if the storage produces changes in scaffold's biomechanical properties. We tested three protocols porcine preservation immersed PBS and alcohol 70%, fridge under cryopreservation. Ninety-six tracheas (12 natura, 84 decellularized) were divided into groups (PBS, alcohol, cryopreservation). Twelve analyzed after...
Abstract Objective: To compare two different wavelengths of the surgical Contact Diode Laser (CDL) for producing a posterior laryngofissure in live pigs. Methods: Anesthetized pigs underwent tracheostomy and an anterior through cervicotomy. They were randomly selected CDL wavelength power (980nm wavelength: Ppeak 10W, 15W, 20W, or 1470nm 3W, 5W, 7W, 10W). At end experiment, laryngotracheal specimen was extracted sent histology morphometry measurements (incision size, depth, area, lateral...
Alteration of airway neuronal function and density bidirectional interaction between immune cells sensory peripheral nerves have been proposed to trigger perpetuate inflammation that contribute asthma severity. To date, few studies analysed neuroplasticity neuroinflammation in tissue asthmatic individuals. We hypothesized the presence these phenomena would be a pathological feature fatal asthma. quantified expression pan-neuronal marker PGP9.5 sensory-derived neuropeptide calcitonin...
Introduction: Humanization in nursing care has gained prominence as an essential strategy to improve the quality of healthcare services. The practice humanization considers not only technical aspects but also emotional, social, and spiritual elements patient, promoting a more comprehensive personcentered approach. Objectives: To analyze contributions emergency, urgent care, intensive services, highlighting its positive impacts on patients’ lives practice. Methodology: This is type...
Abstract To compare two different wavelengths of the surgical contact diode laser (CDL) for producing a posterior laryngofissure in in-vivo pigs. Anesthetized pigs underwent tracheostomy and an anterior through cervicotomy. They were randomly selected CDL wavelength Power, according to peak Power set at device (980nm wavelength: Ppeak power 10 W, 15 20 or 1470 nm 3 5 7 W). At end experiment, laryngotracheal specimen was extracted sent histology morphometry measurements (incision size, depth,...
<b>Background:</b> The alarmin interleukin-33 (IL-33) and its receptor ST2 are suggested contributors to the immunopathology of COVID-19. However, little is known about distribution this pathway in diseased lung tissues. <b>Aims:</b> To reveal spatiotemporal expression patterns IL-33 soluble (sST2) membrane (mST2) variants across COVID-19-inflicted pathologies. <b>Methods:</b> Distal tissue from 20 fatal COVID-19 cases were obtained by minimally invasive autopsy technique. Non-diseased...