A5039465705- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Metabolomics and Mass Spectrometry Studies
- Electrochemical Analysis and Applications
- Advanced Chemical Sensor Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Methods in Pharmaceuticals
- Mass Spectrometry Techniques and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Analytical Chemistry and Sensors
- Trypanosoma species research and implications
- Biochemical Analysis and Sensing Techniques
- Dye analysis and toxicity
- Research on Leishmaniasis Studies
- Analytical chemistry methods development
- Electrostatics and Colloid Interactions
- Water Quality Monitoring and Analysis
- Pesticide Residue Analysis and Safety
- Forensic Toxicology and Drug Analysis
- Biosensors and Analytical Detection
- Protein Interaction Studies and Fluorescence Analysis
- Advanced biosensing and bioanalysis techniques
- Paraquat toxicity studies and treatments
- Phytochemistry Medicinal Plant Applications
Universidade de São Paulo
2016-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2022
ZHAW Zurich University of Applied Sciences
2019
Hospital Universitário da Universidade de São Paulo
2012
Imperial College London
2012
Universidade Federal de São Paulo
2010
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2010
Universidade Cruzeiro do Sul
2008
Universidade Federal de Santa Catarina
2008
Universidade de Brasília
2000
The prevalence of marijuana use among pregnant women is high. However, the effects on gestation and fetal development are not well known. Epidemiological experimental studies present conflicting results because route administration, dose, time exposure, species used, how Cannabis toxicity tested (prepared extracts, specific components, or by pyrolysis). In this study, we experimentally investigated maternal inhalation sativa smoke representing as nearly possible real world conditions human...
"Chimarrão" and "tererê" are maté (dried, toasted milled Ilex paraguariensis leaves stemlets) beverages widely consumed in South America. This paper describes the application of HPLC-DAD-ESI/MS method for identification quantification caffeoylquinic acids (CQA), flavonol glycosides purine alkaloids these beverages. The beverage samples were prepared from commercial lots Southern Brazil. acids, 4,5-diCQA, 3-CQA, 5-CQA, 4-CQA major compounds, having 238-289, 153-242, 183-263, 123-188 μg/mL,...
Este ensaio teórico problematiza a contratação de auxiliares para Educação Infantil, considerando indissociabilidade entre cuidar e educar desvalorização que esses profissionais enfrentam. A pesquisa questiona por há distinção docentes em uma mesma turma, quando ambos desempenham funções interligadas. discussão é embasada autores abordam relação professor auxiliar (Conceição, 2010; Chamarelli & Kramer, 2013; Pinheiro, 2017) o binômio cuidar-educar (Tiriba, 2005; Sayão, 2010). Além disso,...
Metabolomics has become an invaluable tool to unveil biology of pathogens, with immediate application chemotherapy. It is currently accepted that there not one single technique capable obtaining the whole metabolic fingerprint a biological system either due their different physical‐chemical properties or concentrations. In this work, we have explored capability capillary electrophoresis mass spectrometry sheathless interface electrospray ionization ( CE ‐ ESI TOF MS ) separate metabolites in...
There is a rising resistance against antimony drugs, the gold-standard for treatment until some years ago. That serious problem due to paucity of drugs in current clinical use. In research reveal how these affect parasite during and unravel underlying basis their resistance, we have employed metabolomics study Leishmania infantum promastigotes. This was accomplished first through untargeted analysis metabolic snapshots treated untreated parasites both resistant responders, utilizing...
A CE-ESI-MS method was developed and validated for the separation quantitative analysis of amino acids (AA) in urine. Experimental parameters related to CE-MS interface, BGE, mass spectrometer (MS) settings were optimized providing a good 27 AA, including isomers L-leucine, L-isoleucine, L-alloisoleucine, less than 30 min. The sheath liquid composed by 0.50% formic acid 60% (v,v) methanol-water delivered at flow rate 5 μL/min. BGE consisted 0.80 mol/L pH 1.96 15% methanol. stacking procedure...
Abstract In this work, a CE equipment, online hyphenated to an IT MS analyzer by linear sheath liquid interface promoting ESI, was used develop method for quantitative determination of amino acids. Under appropriate conditions (BGE composition, 0.8% HCOOH, 20% CH 3 OH; 60% methanol; V ESI , +4.50 kV), analytical curves all acids from 80 mg/L were recorded presenting acceptable linearity ( r >0.99). LODs in the range 16–172 μmol/L obtained. BSA, model protein, submitted different...
The aim of this work was to develop a simple and fast method for the detection paraquat in oral fluid, plasma, urine by capillary electrophoresis with diode-array (CE-DAD), diagnose acute poisoning related herbicide. use fluid analytical toxicology has been established drugs abuse, but not diagnosis pesticides poisoning. Oral collected without stimulation using absorbent cotton swabs. Sample preparation included diluting, vortex mixing, centrifuging biological ethyl as internal standard....
Combining data from multiple analytical platforms is essential for comprehensive study of the molecular phenotype (metabotype) a given biological sample. The metabolite profiles generated are intrinsically dependent on platforms, each requiring optimization instrumental parameters, separation conditions, and sample extraction to deliver maximal information. An in-depth evaluation protocols characterizing metabolome hepatobiliary fluke Fasciola hepatica , using ultra performance liquid...
Abstract In this work, two methods were developed to determine herbicides paraquat, glyphosate, and aminomethylphosphonic acid ( AMPA ) in marijuana samples by capillary electrophoresis. For paraquat analysis, sample was extracted with aqueous acetic solution analyzed zone electrophoresis direct UV detection. The running electrolyte 50 mmol/L phosphate buffer (p H 2.50). glyphosate , indirect / VIS detection used, as these substances do not present chromophoric groups. Samples 5 hydrochloric...
A novel green ultra high performance liquid chromatography (UHPLC) method was validated and estimated the measurement uncertainty for simultaneous determination of metoprolol tartrate (MET) hydrochlorothiazide (HCT) in binary tablet. Zorbax® SB-C18 column with isocratic elution (flow rate 0.9 mL min−1) at 25°C used. Analytes HCT degradation product were separated approximately 1 min using acetonitrile:water:triethylamine (17:83:0.2, v/v) as mobile phase. Analytical curves linear (R2 > 0.99...
SEPARATION MECHANISMS IN CAPILLARY ELECTROPHORESIS.Since its inception in the 80's, capillary electrophoresis has matured into a well established technique for separation and analysis of complex samples.One strongest aspects is ability to handle materials from diversity chemical classes, ranging few millions Daltons.This only possible because several modes can be performed single format.In this work, relevant zone three (free solution, micellar gel), isoelectric focusing isotachophoresis are...