A5016808090- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Biochemical and Molecular Research
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Galectins and Cancer Biology
- Adenosine and Purinergic Signaling
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Polyamine Metabolism and Applications
- Helminth infection and control
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Nitric Oxide and Endothelin Effects
- Calcium signaling and nucleotide metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Insect symbiosis and bacterial influences
- Cardiomyopathy and Myosin Studies
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Insect Pest Control Strategies
- Lipid metabolism and biosynthesis
Universidade de São Paulo
2015-2025
Universidade Cidade de São Paulo
2014
National Council for Scientific and Technological Development
2012
Instituto Biológico
2011
Universidade Brasil
2000-2009
Instituto de Botânica
2006-2008
Universidade Federal de São Paulo
1999
New York University
1983
Columbia University Irving Medical Center
1983
Fundação de Amparo à Pesquisa do Estado de São Paulo
1974
Trypomastigote forms of Trypanosoma cruzi, the causative agent Chagas Disease, shed extracellular vesicles (EVs) enriched with glycoproteins gp85/trans-sialidase (TS) superfamily and other α-galactosyl (α-Gal)-containing glycoconjugates, such as mucins. Here, purified from T. cruzi strains (Y, Colombiana, CL-14 YuYu) were quantified according to size, intensity concentration. Qualitative analysis revealed differences in their protein contents. Later, those polymorphisms evaluated modulation...
Trypanosoma cruzi causing Chagas' disease needs to invade host cells complete its life cycle. Macromolecules on cell surfaces such as laminin, thrombospondin, heparan sulfate, and fibronectin are believed be important in mediating parasite-host adhesions the invasion process of by parasite. The SELEX technique (systematic evolution ligands byexponential enrichment) was used evolve nuclease-resistant RNA (aptamer = fit) that bind with affinities 40–400 nm parasite receptors for matrix...
Using as the host cell, a proline-requiring mutant of Chinese hamster ovary cell (CHO-K1), it was possible to arrest differentiation amastigote forms Trypanosoma cruzi at intermediate intracellular epimastigote-like stage. Complete trypomastigote stage obtained by addition l-proline medium. This effect more pronounced using T. CL-14 clone that differentiates fully 33°C (permissive temperature) and poorly 37°C (restrictive temperature). A synchronous inside host-cell is then temperature...
ABSTRACT The serodiagnosis of human tegumentary leishmaniasis (TL) presents some problems, such as the low level antileishmanial antibodies found in most patients, well cross-reactivity subjects infected by other trypanosomatids. In present study, an immunoproteomic approach was performed aimed at identification antigens total extracts stationary-phase promastigote and amastigote-like forms Leishmania ( Viannia ) braziliensis using sera from TL patients. With purpose reducing identified...
The infective trypomastigote stage ofTrypanosoma cruzi expresses a set of surface glycoproteins that are known collectively as Tc85 and belong to the gp85/trans-sialidase supergene family. A member this family, Tc85–11, with adhesive properties laminin cell surfaces was recently cloned. In report, Tc85–11 domain for binding its corresponding receptor on epithelial LLC-MK2are described. Using synthetic peptides carboxyl-terminal segment, we show mammalian cell-binding colocalizes most...
Trypanosoma cruzi, the agent of Chagas' disease, alternates between different morphogenetic stages that face distinct physiological conditions in their invertebrate and vertebrate hosts, likely availability glucose. While glucose transport is well characterized epimastigotes T. nothing known about how mammalian acquire this molecule. Herein activity expression were analyzed three developmental present cycle cruzi. The infective trypomastigotes showed highest (V(max)=5.34+/-0.54 nmol/min per...
SYNOPSIS Epimastigotes of Trypanosoma cruzi obtained in culture agglutinate readily with low concentrations concanavalin A (Con A). Agglutination was linear time up to 10 min providing that the initial cell density greater than 1 × 8 cells/ml. Under these conditions, percentage agglutination dependent on Con concentration. inhibited by α‐methyl D‐mannoside, α‐D‐mannose, and α‐D‐glucose. Pretreatment cells trypsin had no effect epimastigote agglutinations. Blood forms (trypomastigotes) T. did...
Background The present study analyzed whether or not the in vitro cultivation for long periods of time pre-isolated Leishmania amazonensis from lesions chronically infected BALB/c mice was able to interfere parasites' infectivity using vivo and experiments. In addition, proteins that presented a significant decrease increase their protein expression content were identified applying proteomic approach. Methodology/Principal Findings Parasites cultured 150 days. Aliquots collected on day 0...
Muscle metaboreflex control in hypertensive subjects has not been described yet. We investigated the integrity of muscle sympathetic nerve activity (MSNA) and blood pressure (BP) never-treated subjects. Eighteen (42 ± 1 years) 22 normotensive (38 were studied. The MSNA was measured by microneurography forearm flow (FBF) venous occlusion plethysmography. BP noninvasively monitored. Baseline significantly increased when compared with normal (34 2 v bursts/min, P < .001). FBF decreased (2.66...
In the past years, extracellular vesicles (EVs) have become an important field of research since EVs been found to play a central role in biological processes. pathogens, are involved several events during host-pathogen interaction, including invasion, immunomodulation, and pathology as well parasite-parasite communication. this report, we summarised infections caused by viruses, bacteria, fungi, protozoa, helminths based on talks discussions carried out International Society for...
Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal spatial triphosphate (ATP) buffering. Here we demonstrate that homologous overexpression of Trypanosoma cruzi arginine improves ability transfectant cells to grow resist nutritional pH stress conditions. The stable transfected parasites showed an increased cell density since day 10 culture, when carbon sources became scarce, resulted 2.5‐fold...