A5073176841- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Parasites and Host Interactions
- Insect Pest Control Strategies
- vaccines and immunoinformatics approaches
- Synthesis and biological activity
- Bioactive Compounds and Antitumor Agents
- Leprosy Research and Treatment
- Insect and Pesticide Research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Phytochemistry and biological activities of Ficus species
- Mycobacterium research and diagnosis
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Leptospirosis research and findings
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Diverse Scientific Research Studies
- Vector-Borne Animal Diseases
- Glutathione Transferases and Polymorphisms
- Advanced Glycation End Products research
Universidade Federal de Minas Gerais
2016-2025
RELX Group (Netherlands)
2018
Abstract: The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity – chitosan (Cs) and chondroitin sulfate (ChS) were used: Cs as positively polymer ChS negatively polymer. (NQ) nanoparticles, chitosan-chondroitin (NQC) sulfate-amphotericin (NQC-AmpB) nanoparticles presented mean particle size of 79, 104, 136 nm,...
Background The present study analyzed whether or not the in vitro cultivation for long periods of time pre-isolated Leishmania amazonensis from lesions chronically infected BALB/c mice was able to interfere parasites' infectivity using vivo and experiments. In addition, proteins that presented a significant decrease increase their protein expression content were identified applying proteomic approach. Methodology/Principal Findings Parasites cultured 150 days. Aliquots collected on day 0...
Abstract Leishmaniases are neglected diseases caused by infection with Leishmania parasites and there currently no prophylactic vaccines. In this study, we designed in silico a synthetic recombinant vaccine against visceral leishmaniasis (VL) called ChimeraT, which contains specific T-cell epitopes from Prohibitin, Eukaryotic Initiation Factor 5a the hypothetical LiHyp1 LiHyp2 proteins. Subcutaneous delivery of ChimeraT plus saponin stimulated Th1 cell-mediated immune response protected mice...
Leishmaniasis, a neglected tropical disease caused by Leishmania species, presents serious public health challenges due to limited treatment options, toxicity, high costs, and drug resistance. In this study, the in vitro potential of malvidin echioidinin is examined as antileishmanial agents against L. amazonensis, braziliensis, infantum, comparing their effects amphotericin B (AmpB), standard drug. Malvidin demonstrated greater potency than across all parasite stages species. Against...
In this study, a recombinant chimeric protein (RCP), which was composed of specific CD4+ and CD8+ T-cell epitopes to murine human haplotypes, evaluated as an immunogen against Leishmania infantum infection in model. BALB/c mice received saline were immunized with saponin or RCP without adjuvant. The results showed that RCP/saponin-vaccinated presented significantly higher levels antileishmanial IFN-γ, IL-12 GM-CSF before after challenge, associated the reduction IL-4 IL-10 mediated...
In this study, a quinoline derivate, clioquinol (5-chloro-7-iodoquinolin-8-ol), was evaluated against Leishmania amazonensis and infantum promastigotes amastigotes. The cytotoxicity in murine macrophages human red blood cells, as well the efficacy treating infected inhibition of infection using pre-treated parasites were also evaluated. Results showed that inhibited L. with effective concentration 50% (EC50 ) values 2.55 ± 0.25 1.44 0.35 μg/mL, respectively, 1.88 0.13 0.98 0.17 μg/mL axenic...
Currently, there is no licensed vaccine to protect against human visceral leishmaniasis (VL), a potentially fatal disease caused by infection with Leishmania parasites. In the current study, recombinant chimeric protein ChimT was developed based on T-cell epitopes identified from immunogenic amastigote proteins LiHyp1, LiHyV, LiHyC and LiHyG. associated adjuvants saponin (Sap) or monophosphoryl lipid A (MPLA) used immunize mice, their immunogenicity protective efficacy were evaluated. Both...
Summary In this work, the effect of vaccination a newly described Leishmania infantum antigenic protein has been studied in BALB /c mice infected with parasite species. The LiHyD was characterized after proteomic screening performed sera from dogs suffering visceral leishmaniasis ( VL ). Its recombinant version expressed, purified and administered to combination saponin. As result 10 weeks challenge using an infective dose L. stationary promastigotes, vaccinated showed lower burdens...
Introduction: Leishmaniasis is a disease with high mortality rates and approximately 1.5 million new cases each year. Despite the approaches advances to fight disease, there are no effective therapies. Methods: Hence, this study aims screen for natural products' structural analogs as drug candidates against leishmaniasis. We applied Computer-aided design (CADD) approaches, such virtual screening, molecular docking, dynamics simulation, mechanics-generalized Born surface area (MM-GBSA)...
The development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, antileishmanial activity Strychnos pseudoquina St. Hil. was investigated pure compounds that presented this biological effect were isolated. An ethyl acetate extract prepared, it proved be effective against Leishmania amazonensis. A bioactivity-guided fractionation performed, two flavonoids identified, quercetin 3-O-methyl ether...
In the present study, two Leishmania infantum hypothetical proteins in amastigote stage, LiHyp1 and LiHyp6, were combined with a promastigote protein, IgE-dependent histamine-releasing factor (HRF); to compose polyproteins vaccine be evaluated against L. infection. Also, antigenicity of three was analyzed, their use for serodiagnosis canine visceral leishmaniasis (CVL) evaluated. The LiHyp1, HRF DNA coding sequences cloned prokaryotic expression vectors recombinant purified. When employed...