A5067888096- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Parasites and Host Interactions
- Insect Pest Control Strategies
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- SARS-CoV-2 detection and testing
- Synthesis and biological activity
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- E-Learning and Knowledge Management
- Insect and Pesticide Research
- Scientific Research and Technology
- Synthesis and Characterization of Heterocyclic Compounds
- Vector-borne infectious diseases
- Biosensors and Analytical Detection
- Mosquito-borne diseases and control
- Bioactive Compounds and Antitumor Agents
- Leprosy Research and Treatment
- Poxvirus research and outbreaks
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Science and Education Research
Universidade Federal de Minas Gerais
2016-2025
Petrobras (Brazil)
2024
Fundação Faculdade de Medicina
2024
Universidade do Extremo Sul Catarinense
2024
Catholic University of Santa María
2022-2023
Universidade Federal do Pará
2019
RELX Group (Netherlands)
2018
University of Florida
2016
Columbus Oncology and Hematology Associates
2016
Reckitt Benckiser (United States)
2016
Leishmania amazonensis is one of the major etiologic agents a broad spectrum clinical forms leishmaniasis and has wide geographical distribution in Americas, which overlaps with areas transmission many other species. The LACK A2 antigens are shared by various was previously shown to induce potent Th1 immune response protection against L. donovani infection BALB/c mice. effective infection, but no significant observed, spite induction response. In an attempt select candidate for American...
Background The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies the sera dogs with asymptomatic symptomatic visceral leishmaniasis (VL). Methodology/Principal Findings Proteins samples were separated two-dimensional electrophoresis (2DE) identified mass spectrometry. A total 550 spots observed 2DE gels, approximately 104 proteins identified. Several stage-specific...
Abstract: The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity – chitosan (Cs) and chondroitin sulfate (ChS) were used: Cs as positively polymer ChS negatively polymer. (NQ) nanoparticles, chitosan-chondroitin (NQC) sulfate-amphotericin (NQC-AmpB) nanoparticles presented mean particle size of 79, 104, 136 nm,...
ABSTRACT The serodiagnosis of human tegumentary leishmaniasis (TL) presents some problems, such as the low level antileishmanial antibodies found in most patients, well cross-reactivity subjects infected by other trypanosomatids. In present study, an immunoproteomic approach was performed aimed at identification antigens total extracts stationary-phase promastigote and amastigote-like forms Leishmania ( Viannia ) braziliensis using sera from TL patients. With purpose reducing identified...
Serum-based ELISA (enzyme-linked immunosorbent assay) has been widely used to detect anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. However, date, no study investigated patient urine as a biological sample SARS-CoV-2 virus-specific An in-house urine-based was developed using recombinant nucleocapsid protein. The presence of antibodies in established, with 94% sensitivity and 100% specificity for the detection anti-SARS-CoV-2 88% paired serum-based ELISA. that...
Abstract: Amphotericin B (AmpB) is active against leishmaniasis, but its use hampered due to high toxicity observed in patients. In this study, a nanoparticles-delivery system for AmpB (NQC-AmpB), containing chitosan and chondroitin sulfate molecules, was evaluated BALB/c mice Leishmania amazonensis . An vivo biodistribution including biochemical toxicological evaluations, performed evaluate the of AmpB. Nanoparticles were radiolabeled with technetium-99m injected mice. The products...
Background The present study analyzed whether or not the in vitro cultivation for long periods of time pre-isolated Leishmania amazonensis from lesions chronically infected BALB/c mice was able to interfere parasites' infectivity using vivo and experiments. In addition, proteins that presented a significant decrease increase their protein expression content were identified applying proteomic approach. Methodology/Principal Findings Parasites cultured 150 days. Aliquots collected on day 0...
Abstract Leishmaniases are neglected diseases caused by infection with Leishmania parasites and there currently no prophylactic vaccines. In this study, we designed in silico a synthetic recombinant vaccine against visceral leishmaniasis (VL) called ChimeraT, which contains specific T-cell epitopes from Prohibitin, Eukaryotic Initiation Factor 5a the hypothetical LiHyp1 LiHyp2 proteins. Subcutaneous delivery of ChimeraT plus saponin stimulated Th1 cell-mediated immune response protected mice...
Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide the most diverse diseases, this review article aims to survey use of these antigens diagnosis, as well discuss main points surrounding antigens. RMPs usually consisting linear, immunodominant, and phylogenetically conserved epitopes, has been applied experimental diagnosis various human animal such leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis,...
Leishmaniasis, a neglected tropical disease caused by Leishmania species, presents serious public health challenges due to limited treatment options, toxicity, high costs, and drug resistance. In this study, the in vitro potential of malvidin echioidinin is examined as antileishmanial agents against L. amazonensis, braziliensis, infantum, comparing their effects amphotericin B (AmpB), standard drug. Malvidin demonstrated greater potency than across all parasite stages species. Against...