A5102909677- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Parasites and Host Interactions
- vaccines and immunoinformatics approaches
- Insect Pest Control Strategies
- Transgenic Plants and Applications
- SARS-CoV-2 detection and testing
- Monoclonal and Polyclonal Antibodies Research
- Bioactive Compounds and Antitumor Agents
- Leprosy Research and Treatment
- Mycobacterium research and diagnosis
- Phytochemical compounds biological activities
- Pharmacological Effects of Natural Compounds
- SARS-CoV-2 and COVID-19 Research
- Synthesis and biological activity
- Diverse Scientific Research Studies
- Occupational and environmental lung diseases
- Tuberculosis Research and Epidemiology
- Herbal Medicine Research Studies
- Leptospirosis research and findings
- Food Allergy and Anaphylaxis Research
- Blood Coagulation and Thrombosis Mechanisms
- Phytochemistry and Bioactivity Studies
Universidade Federal de Minas Gerais
2016-2025
Abstract: The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity – chitosan (Cs) and chondroitin sulfate (ChS) were used: Cs as positively polymer ChS negatively polymer. (NQ) nanoparticles, chitosan-chondroitin (NQC) sulfate-amphotericin (NQC-AmpB) nanoparticles presented mean particle size of 79, 104, 136 nm,...
Abstract: Amphotericin B (AmpB) is active against leishmaniasis, but its use hampered due to high toxicity observed in patients. In this study, a nanoparticles-delivery system for AmpB (NQC-AmpB), containing chitosan and chondroitin sulfate molecules, was evaluated BALB/c mice Leishmania amazonensis . An vivo biodistribution including biochemical toxicological evaluations, performed evaluate the of AmpB. Nanoparticles were radiolabeled with technetium-99m injected mice. The products...
Background The present study analyzed whether or not the in vitro cultivation for long periods of time pre-isolated Leishmania amazonensis from lesions chronically infected BALB/c mice was able to interfere parasites' infectivity using vivo and experiments. In addition, proteins that presented a significant decrease increase their protein expression content were identified applying proteomic approach. Methodology/Principal Findings Parasites cultured 150 days. Aliquots collected on day 0...
Abstract Leishmaniases are neglected diseases caused by infection with Leishmania parasites and there currently no prophylactic vaccines. In this study, we designed in silico a synthetic recombinant vaccine against visceral leishmaniasis (VL) called ChimeraT, which contains specific T-cell epitopes from Prohibitin, Eukaryotic Initiation Factor 5a the hypothetical LiHyp1 LiHyp2 proteins. Subcutaneous delivery of ChimeraT plus saponin stimulated Th1 cell-mediated immune response protected mice...
The development of cost-effective prophylactic strategies to prevent leishmaniasis has become a high-priority. present study used the phage display technology identify new immunogens, which were evaluated as vaccines in murine model visceral (VL). Epitope-based represented by phage-fused peptides that mimic Leishmania infantum antigens, selected according their affinity antibodies from asymptomatic and symptomatic VL dogs' sera.Twenty clones after three selection cycles, means vitro assays...
In this study, a recombinant chimeric protein (RCP), which was composed of specific CD4+ and CD8+ T-cell epitopes to murine human haplotypes, evaluated as an immunogen against Leishmania infantum infection in model. BALB/c mice received saline were immunized with saponin or RCP without adjuvant. The results showed that RCP/saponin-vaccinated presented significantly higher levels antileishmanial IFN-γ, IL-12 GM-CSF before after challenge, associated the reduction IL-4 IL-10 mediated...
Currently, there is no licensed vaccine to protect against human visceral leishmaniasis (VL), a potentially fatal disease caused by infection with Leishmania parasites. In the current study, recombinant chimeric protein ChimT was developed based on T-cell epitopes identified from immunogenic amastigote proteins LiHyp1, LiHyV, LiHyC and LiHyG. associated adjuvants saponin (Sap) or monophosphoryl lipid A (MPLA) used immunize mice, their immunogenicity protective efficacy were evaluated. Both...
Leishmania chagasi and amazonensis are the etiologic agents of different clinical forms human leishmaniasis in South America. In an attempt to select candidate antigens for a vaccine protecting against species, efficacy vaccination using ribosomal proteins saponin as adjuvant was examined BALB/c mice challenge infection with both parasite species. Mice vaccinated purified from infantum plus showed specific production IFN-γ, IL-12 GM-CSF after vitro stimulation L. proteins. Vaccinated...
LiHyV is an antigenic hypothetical protein present in both promastigote and amastigote stages of Leishmania infantum, which was recently identified by immunoproteomic approach. A recombinant version this (rLiHyV) evaluated as a diagnostic marker for canine VL (CVL). In addition, the prophylactic efficacy rLiHyV protein, two its CD8+ T cell epitopes, has been analyzed murine model visceral leishmaniasis (VL). Initially, ELISA technique serodiagnosis CVL. Secondly, vaccines composed chemically...
Summary In this work, the effect of vaccination a newly described Leishmania infantum antigenic protein has been studied in BALB /c mice infected with parasite species. The LiHyD was characterized after proteomic screening performed sera from dogs suffering visceral leishmaniasis ( VL ). Its recombinant version expressed, purified and administered to combination saponin. As result 10 weeks challenge using an infective dose L. stationary promastigotes, vaccinated showed lower burdens...
The development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, antileishmanial activity Strychnos pseudoquina St. Hil. was investigated pure compounds that presented this biological effect were isolated. An ethyl acetate extract prepared, it proved be effective against Leishmania amazonensis. A bioactivity-guided fractionation performed, two flavonoids identified, quercetin 3-O-methyl ether...
In the present study, two Leishmania infantum hypothetical proteins in amastigote stage, LiHyp1 and LiHyp6, were combined with a promastigote protein, IgE-dependent histamine-releasing factor (HRF); to compose polyproteins vaccine be evaluated against L. infection. Also, antigenicity of three was analyzed, their use for serodiagnosis canine visceral leishmaniasis (CVL) evaluated. The LiHyp1, HRF DNA coding sequences cloned prokaryotic expression vectors recombinant purified. When employed...