A5002279455- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Leptospirosis research and findings
- T-cell and Retrovirus Studies
- vaccines and immunoinformatics approaches
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Hepatitis Viruses Studies and Epidemiology
- Reproductive System and Pregnancy
Universidade Federal de Minas Gerais
2016-2021
Abstract Leishmaniases are neglected diseases caused by infection with Leishmania parasites and there currently no prophylactic vaccines. In this study, we designed in silico a synthetic recombinant vaccine against visceral leishmaniasis (VL) called ChimeraT, which contains specific T-cell epitopes from Prohibitin, Eukaryotic Initiation Factor 5a the hypothetical LiHyp1 LiHyp2 proteins. Subcutaneous delivery of ChimeraT plus saponin stimulated Th1 cell-mediated immune response protected mice...
Background: Leishmaniases are neglected diseases caused by infection with Leishmania parasites and there no human vaccines in use routinely. The purpose of this study was to examine the immunogenicity ChimeraT, a novel synthetic recombinant vaccine against visceral leishmaniasis (VL), incorporated into human-compatible liposome formulation. Methods: BALB/c mice were immunized subcutaneously ChimeraT/liposome vaccine, ChimeraT/saponin adjuvant, or ChimeraT/saline immune responses examined...
The development of a vaccine for the prevention visceral leishmaniasis (VL) still represents significant unmet medical need. A human can be found if one takes into consideration that many people living in endemic areas disease are infected but do not develop active VL, including those subjects with subclinical or asymptomatic infection. In this study, phage display was used to select phage-exposed peptides were specific immunoglobulin G (IgG) antibodies from and symptomatic VL patients,...
Abstract In the current study, phage-exposed mimotopes as targets against tegumentary leishmaniasis (TL) were selected by means of bio-panning cycles employing sera TL patients and healthy subjects, besides immune stimulation peripheral blood mononuclear cells (PBMCs) collected from untreated treated subjects. The clones evaluated regarding their specific interferon- γ (IFN- ) interleukin-4 (IL-4) production in vitro cultures, selectivity specificity values calculated, those presenting best...
The co-infection between visceral leishmaniasis (VL) and human immunodeficiency virus (HIV) has increased in several countries the world. current serological tests are not suitable since they present low sensitivity to detect most of VL/HIV cases, a more precise diagnosis should be performed. In this context, study, an immunoproteomics approach was performed using Leishmania infantum antigenic extracts VL, HIV patients sera, besides healthy subjects samples; aiming identify markers for these...