A5077594914- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Acute Myeloid Leukemia Research
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Metabolomics and Mass Spectrometry Studies
- Platelet Disorders and Treatments
- Chemokine receptors and signaling
- Lymphatic System and Diseases
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Metastasis and carcinoma case studies
- Multiple Myeloma Research and Treatments
- Protease and Inhibitor Mechanisms
- Bone health and treatments
- Sarcoma Diagnosis and Treatment
- Vascular Tumors and Angiosarcomas
Instituto de Medicina Molecular João Lobo Antunes
2006-2025
Instituto Português de Oncologia Francisco Gentil
2004-2024
University of Lisbon
2015-2024
Universidad ORT Uruguay
2024
Universidade Federal de Minas Gerais
2024
Escola Superior de Enfermagem do Porto
2020
Vale (Brazil)
2019
Faculdade São Leopoldo Mandic
2018
Universidade Federal de Santa Catarina
2018
National Legal Medicine Institute
2008-2014
Abstract —Bone marrow (BM)–derived circulating endothelial precursor cells (CEPs) are thought to play a role in postnatal angiogenesis. Emerging evidence suggests that angiogenic stress of vascular trauma may induce mobilization CEPs the peripheral circulation. In this regard, we studied kinetics CEP two groups patients who experienced acute insult secondary burns or coronary artery bypass grafting (CABG). both burn and CABG patients, there was consistent, rapid increase number CEPs,...
Tyrosine kinase receptors for angiogenic factors vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) are expressed not only by cells but also subsets of hematopoietic stem (HSCs). To further define their role in the regulation postnatal hematopoiesis vasculogenesis, VEGF Ang-1 plasma levels were elevated injecting recombinant protein or adenoviral vectors expressing soluble VEGF165, matrix-bound VEGF189, into mice. induced a rapid mobilization HSCs receptor (VEGFR)2+...
Emerging data suggest that VEGF receptors are expressed by endothelial cells as well hematopoietic stem cells. Therefore, we hypothesized functional may also be in malignant counterparts of such leukemias. We demonstrate certain leukemias not only produce but express VEGFR-2 vivo and vitro, resulting the generation an autocrine loop support leukemic cell survival proliferation. Approximately 50% freshly isolated mRNA protein for VEGFR-2. VEGF(165) induced phosphorylation increased...
Trypanosoma brucei is an extracellular parasite that causes sleeping sickness. In mammalian hosts, trypanosomes are thought to exist in two major niches: early infection, they populate the blood; later, breach blood-brain barrier. Working with a well-established mouse model, we discovered adipose tissue constitutes third reservoir for T. brucei. Parasites from tissue, here termed forms (ATFs), can replicate and were capable of infecting naive animal. ATFs transcriptionally distinct...
The chemokine stroma-derived factor-1 (SDF-1) is produced within the bone marrow and mediates chemokinesis chemotaxis on a variety of cell types that express CXCR4 receptor. SDF-1-responsive include monocytes macrophages, B T lymphocytes, platelets megakaryocytes, CD34+ cells, including both hematopoietic progenitors stem cells. We have used intravenous injection replication-incompetent adenovector expressing SDF-1 gene to elevate serum levels in Balb/c SCID mice. Within 3 5 days there was...
Antiangiogenic agents block the effects of tumor-derived angiogenic factors (paracrine factors), such as vascular endothelial growth factor (VEGF), on cells (EC), inhibiting solid tumors. However, whether inhibition angiogenesis also may play a role in liquid tumors is not well established. We recently have shown that certain leukemias only produce VEGF but selectively express functional receptors (VEGFRs), VEGFR-2 (Flk-1, KDR) and VEGFR1 (Flt1), resulting generation an autocrine loop. Here,...
Lipids and cholesterol in particular, have long been associated with breast cancer (BC) onset progression. However, the causative effects of elevated lipid levels remain largely undisclosed were subject present study.We took advantage well-established vitro vivo models enrichment to exploit mechanism involved LDL-cholesterol favouring BC growth invasiveness. We analyzed its that mimic different subtypes stages.Our data show (but not HDL-cholesterol) promotes cells proliferation, migration...
Among women, breast cancer (BC) is the leading and most common cause of cancer-related death between 30 69 years. Although lifestyle diet are considered to have a role in global BC incidence pattern, specific influence dyslipidemia onset progression not yet completely understood. Fasting lipid profile (total cholesterol, LDL-C, HDL-C, triglycerides) was prospectively assessed 244 women with who were enrolled according pre-set inclusion criteria: diagnosis non-hereditary invasive ductal...
In the last decade there have been multiple studies concerning contribution of endothelial progenitor cells (EPCs) to new vessel formation in different physiological and pathological settings. The process by which EPCs contribute adults is termed postnatal vasculogenesis occurs via four inter-related steps. They must respond chemoattractant signals mobilize from bone marrow peripheral blood; home on sites formation; invade migrate at same sites; differentiate into mature (ECs) and/or...
In a murine model of breast cancer, IL-12 therapy exerts potent anti-angiogenic effects which contribute to tumor regression. After 7 days treatment, levels VEGF protein decline markedly and are undetectable at 14 days. This is accompanied by fall in MMP-9 and, as the tumors regress, an increase its natural inhibitor, TIMP-1. A cell line established from primary produced vitro. IFN-γ reduced production over 24-hr period vitro, suggesting that IL-12-induced may be responsible for vivo. There...