A5072323984- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Insect Resistance and Genetics
- Biofuel production and bioconversion
- Venomous Animal Envenomation and Studies
- Enzyme Production and Characterization
- Phytase and its Applications
- Bacteriophages and microbial interactions
- Agricultural pest management studies
- Silk-based biomaterials and applications
- Insect and Pesticide Research
- Microbial Metabolic Engineering and Bioproduction
- Antifungal resistance and susceptibility
- Protein Interaction Studies and Fluorescence Analysis
- Protein Hydrolysis and Bioactive Peptides
- Peptidase Inhibition and Analysis
- Botanical Research and Chemistry
- Nanoparticle-Based Drug Delivery
- RNA and protein synthesis mechanisms
- Trypanosoma species research and implications
- Catalysis for Biomass Conversion
- Microbial Metabolites in Food Biotechnology
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
Universidade de Brasília
2015-2024
Brazilian Agricultural Research Corporation
2020
Utah State University
2020
Museu da Amazonia
2014
Proteasome inhibitors have been described as an important target for cancer therapy due to their potential regulate the ubiquitin-proteasome system in degradation pathway of cellular proteins. Here, we reported effects a Bowman-Birk-type protease inhibitor, Black-eyed pea Trypsin/Chymotrypsin Inhibitor (BTCI), on proteasome 20S MCF-7 breast cells and catalytic activity purified from horse erythrocytes, well structural analysis BTCI-20S complex. In vitro experiments confocal microscopy showed...
The incidence of fungal infections has been increasing in the last decades, while number available antifungal classes remains same. natural and acquired resistance some species to therapies, associated with high toxicity these drugs on present scenario makes an imperative search for new, more efficient less toxic therapeutic choices. Antimicrobial peptides (AMPs) are a potential class antimicrobial consisting evolutionarily conserved multifunctional molecules both microbicidal...
Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide the most diverse diseases, this review article aims to survey use of these antigens diagnosis, as well discuss main points surrounding antigens. RMPs usually consisting linear, immunodominant, and phylogenetically conserved epitopes, has been applied experimental diagnosis various human animal such leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis,...
Recently, defense peptides that are able to act against several targets have been characterized. The present work focuses on structural and functional evaluation of the peptide analogue Pa-MAP, previously isolated as an antifreeze from Pleuronectes americanus. Pa-MAP showed activities different such tumoral cells in culture (CACO-2, MCF-7 HCT-116), bacteria (Escherichia coli ATCC 8739 Staphylococcus aureus 25923), viruses (HSV-1 HSV-2) fungi (Candida parapsilosis 22019, Trichophyton...
Oligopeptidase B, a processing enzyme of the prolyl oligopeptidase family, is considered as an important virulence factor in trypanosomiasis. Trypanosoma cruzi B (OPBTc) involved host cell invasion by generating Ca2+-agonist necessary for recruitment and fusion lysosomes at site parasite attachment. The underlying mechanism remains unknown further structural functional characterization OPBTc may help clarify its physiological function lead to development new therapeutic molecules treat...
In the present manuscript, a novel fluorescent chalcone derivative is synthesized and its photophysical properties are fully characterized. The designed fluorophore applied as probe to study protein-dye interactions with bovine serum albumin. Circular dichroism gave interesting results on thermodynamics of interaction. NMR spectroscopy, especially relaxation measurements, revealed atoms in that interacts protein upon binding. Molecular docking calculations indicate most favourable binding...
The kappa-KTx family of peptides, which is the newest K⁺-channel blocker from scorpion venom, present in scorpions families Scorpionidae and Liochelidae. Differently other KTx families, three-dimensional structure known kappa-KTxs toxins formed by two parallel α-helices linked disulfide bridges. Here, characterization a new peptide, designated 2.5, derived Liochelidae Opisthacanthus cayaporum, described. This peptide was purified HPLC found to be identical OcyC8, predicted mature sequence...
Amphibian skin secretions are abundant in bioactive compounds, especially antimicrobial peptides. These molecules generally cationic and rich hydrophobic amino acids, have an amphipathic structure adopt α-helical conformation when contact with microorganisms membranes. In this work, we purified characterized Figainin 1, a novel antiproliferative peptide from the cutaneous secretion of frog Boana raniceps. 1 is eighteen acid residues-rich leucine isoleucine, amidated C-terminus-and adopts...
Protease inhibitors (PIs) are present in a wide variety of living organisms including microorganisms, plants, and animals. These molecules act as regulators endogenous proteolytic activity, participants many developmental processes hosts defense components. Because their important role organisms, PIs have been extensively studied order to allow better understanding structural functional properties. Intensive works reported that proteinaceous potential compounds several distinct economical...
An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography. The enzyme activated <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msup><mml:msub><mml:mtext>NH</mml:mtext><mml:mn>4</mml:mn></mml:msub><mml:mo>+</mml:mo></mml:msup></mml:math> inhibited Cu +2 Hg . Significant biochemical structural discrepancies between wild-type...
Hepatitis B is a liver inflammation caused by hepatitis virus (HBV) and can be diagnosed in clinical stage core antibody from IgM class (anti-HBcIgM). IgG (Anti-HBcIgG) appears quickly after IgM, reaching high titers chronic hepatitis, remains even cure. Since (anti-HBc) the first identified sometimes only marker detected during course of infection, it used both to indicate HBV acute infection (anti-HBc-IgM) identify individuals who have come into contact with (anti-HBc-IgG). In this work we...
Commercial interest in plant cell wall degrading enzymes (PCWDE) is motivated by their potential for energy or bioproduct generation that reduced dependency on non-renewable (fossil-derived) feedstock. Therefore, underlying work analysed the Penicillium chrysogenum isolate PCWDE production employing different biomass as a carbon source. Among produced enzymes, three xylanase isoforms were observed culture filtrate containing sugarcane bagasse. Xylanase (PcX1) presenting 35 kDa molecular mass...
Hepatitis C virus (HCV) has emerged as the major pathogen of liver diseases in recent years leading to worldwide blood-transmitted chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Accurate diagnosis for differentiation hepatitis from other viruses is thus pivotal importance proper treatment. In this work we developed a recombinant multiepitope protein (rMEHCV) diagnostic purposes based on conserved immunodominant epitopes core , NS3, NS4A, NS4B, NS5 regions polyprotein genotypes...
S-layers commonly cover archaeal cell envelopes and are composed of proteins that self-assemble into a paracrystalline surface structure. Despite their detection in almost all archaea, there few reports investigating the structural properties these proteins, with no exploring this topic for halophilic S-layers. The objective present study was to investigate secondary tertiary organization Haloferax volcanii S-layer protein. Such investigations were performed using circular dichroism,...
In recent years, the number of new antimicrobial drugs launched on market has decreased considerably even though there been an increase in resistant microbial strains. Thus, resistance become a serious public health problem. Amphibian skin secretions are rich source host defense peptides, which generally cationic and hydrophobic molecules, with broad-spectrum activity. this study, one novel multifunctional peptide was isolated from secretion Chaco tree frog, Boana raniceps. Figainin 2...
Peptidase inhibitors (PIs) have been broadly studied due to their wide therapeutic potential for human diseases. A potent trypsin inhibitor from Tityus obscurus scorpion venom was characterized and named ToPI1, with 33 amino acid residues three disulfide bonds. The X-ray structure of the ToPI1:trypsin complex, in association mass spectrometry data, indicate a sequential set events: complex formation Lys32 S1 pocket, C-terminal residue Ser33 cleavage, cyclization ToPI1 via peptide bond...