A5062281474- Antimicrobial Peptides and Activities
- Protein Hydrolysis and Bioactive Peptides
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Marine Sponges and Natural Products
- Aquaculture disease management and microbiota
- Antifungal resistance and susceptibility
- Advanced Nanomaterials in Catalysis
- Immune Response and Inflammation
- Microbial Natural Products and Biosynthesis
- Antimicrobial agents and applications
- Invertebrate Immune Response Mechanisms
- Lipid Membrane Structure and Behavior
- Pediatric health and respiratory diseases
- Bacteriophages and microbial interactions
- Membrane-based Ion Separation Techniques
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Ion Transport and Channel Regulation
- Silk-based biomaterials and applications
- Computational Drug Discovery Methods
- Antibiotic Use and Resistance
- Insect Resistance and Genetics
- Nicotinic Acetylcholine Receptors Study
- Nanocluster Synthesis and Applications
University of Havana
2016-2025
Universität Ulm
2021
Faculty (United Kingdom)
2017
Universidade Católica de Brasília
2016
Cm-p5, as well its cyclic monomer and dimeric analogues are known for their antifungal, antibacterial, antiviral, antibiofilm activities. Previously, our cyclization method generated a mixture of peptides that were difficult to separate, which was then improved by selective synthesis the parallel dimer differentiation from antiparallel comparing retention times in RP-HPLC. Here, we developed more reliable identification method, comprising chymotrypsin proteolytic degradation sequencing...
Background/Objectives: Cm-p5 and its cyclic monomeric dimeric analogues are known for their antifungal, antibacterial, antiviral, antibiofilm activities. Previously, our cyclization method produced a mixture of peptides that were difficult to separate, which was then improved by selective synthesis the parallel dimer differentiation from antiparallel comparison retention times in RP-HPLC. Methods: Here, we developed more reliable identification identification, included chymotrypsin...
Antimicrobial peptides have been found in mollusks and other sea animals. In this report, a crude extract of the marine snail Cenchritis muricatus was evaluated against human pathogens responsible for multiple deleterious effects diseases. A peptide 1485.26 Da purified by reversed-phase HPLC functionally characterized. This trypsinized sequenced MS/MS technology, sequence (SRSELIVHQR), named Cm-p1 recovered, chemically synthesized demonstrated capacity to prevent development yeasts...
Antimicrobial peptides form part of the first line defense against pathogens for many organisms. Current treatments fungal infections are limited by drug toxicity and pathogen resistance. Cm-p5 (SRSELIVHQRLF), a peptide derived from marine mollusk Cenchritis muricatus Cm-p1, has significantly increased fungistatic activity pathogenic Candida albicans (minimal inhibitory concentration, 10 µg/ml; EC50, 1.146 µg/ml) while exhibiting low toxic effects cultured mammalian cell line. as...
Growth in biofilms as a fascinating and complex microbial lifestyle has become widely accepted one of the key features pathogenic microbes, to successfully express their full virulence potential environmental persistence. This also increases threat posed by Candida auris, which high intrinsic ability persist on abiotic surfaces including those surgical instruments medical tubing. In previous study, cyclic helical-stabilized analogues antifungal peptide Cm-p5 were designed synthetized, proved...
Mollusks have been widely investigated for antimicrobial peptides because their humoral defense against pathogens is mainly based on these small biomolecules. In this report, we describe the identification of three novel from marine mollusk Nerita versicolor. A pool N. versicolor was analyzed with nanoLC-ESI-MS-MS technology, and potential (Nv-p1, Nv-p2 Nv-p3) were identified bioinformatical predictions selected chemical synthesis evaluation biological activity. Database searches showed that...
A new solid-phase protocol for the synthesis of N-substituted and tetrazolo peptides is described. The strategy relies on combination aminocatalysis-mediated on-resin Ugi reactions peptide couplings N-alkylation at selected sites, including N-terminal double lipidation, simultaneous lipidation/biotinylation, steroid/lipid conjugation via tetrazole ring formation. four-component were enabled by transimination steps prior to addition acid isocyanide components. proved be suitable feasible...
Diseases caused by bacterial and fungal pathogens are among the major health problems in world. Newer antimicrobial therapies based on novel molecules urgently need to be developed, this includes peptides. In spite of potential peptides, very few them were able successfully developed into therapeutics. The they present molecule stability, toxicity host cells, production costs. A strategy overcome these obstacles is conjugation nanomaterial preparations. activity different types nanoparticles...
Following the information obtained by a rational design study, cyclic and helical-stabilized analogue of peptide Cm-p5 was synthetized. The monomer showed an increased activity in vitro against Candida albicans parapsilosis, compared to Cm-p5. Initially, 14 mutants were synthesized following improve antifungal pharmacological properties. Antimicrobial testing that lost each these analogues, suggesting, as main conclusion, Glu–His salt bridge could stabilize helical conformation during...
Antimicrobial peptides (AMPs) are biomolecules with antimicrobial activity against a broad group of pathogens. In the past few decades, AMPs have represented an important alternative for treatment infectious diseases. Their isolation from natural sources has been widely investigated. this sense, mollusks promising organisms identification given that their immune system mainly relies on innate response. report, we characterized peptide fraction Cuban freshwater snail Pomacea poeyana (Pilsbry,...
Two birds in one shot: oligopeptides can be cyclized and lipidated step with multicomponent reactions.
Recently two peptides isolated from the Cuban freshwater snail Pomacea poeyana (Pilsbry, 1927) were described to have antimicrobial activity against bacterial pathogens. Here we show considerable activities of Pom-1 and Pom-2 reduce viability C. albicans, parapsilosis less common species auris measured as decrease metabolic in resazurin reduction assay for planktonic cells. Although these low, turned out be highly potent inhibitors biofilm formation three Candida tested. Whereas was slightly...
The diminishing portfolio of mankind's available antibiotics urges science to develop novel potent drugs. Here, we present a peptide fitting the typical blueprint amphipathic and membrane-active antimicrobial peptides, denominated C14R. This 2 kDa consists 16 amino acid residues, with seven being either hydrophobic, aromatic, or non-polar, nine polar positively charged, strictly separated on opposite sides predicted α-helix. affinity C14R
<title>Abstract</title> <italic>Candida albicans</italic> infections are a global health thread and challenge healthcare environments due to acquired resistances against prominent antifungals like amphotericin B fluconazole, which additionally have severe adverse effects. The peptide Pom-1 originally isolated from the freshwater mollusk <italic>Pomacea poeyana</italic>, its derivatives Pom-1A-F proven their potential biofilms of clinical <italic>C. albicans </italic>isolates were suspected...
Candida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite clear difficulties of control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for control. In this report, different concentrations antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers drug delivery. The characterized by mass spectrometry confirming presence on scaffold. Atomic force microscopy and...
Abstract The pathogenic yeast Candida auris has received increasing attention due to its ability cause fatal infections, resistance toward important fungicides, and persist on surfaces including medical devices in hospitals. To brace health care systems for this considerable risk, alternative therapeutic approaches such as antifungal peptides are urgently needed. In clinical wound care, a significant focus been directed novel surgical (wound) dressings first defense lines against C. ....
The marine ecosystem is able to provide enormous biomolecule diversity that could be used for treatment of various diseases. In this highly competitive environment, organisms need chemical barriers reduce or avoid microorganism contamination. Among the molecules protect these animals antimicrobial peptides (AMPs) are included. present study, crude extracts coral specimens Carijoa riisei, Muriceopsis sulphurea, Neospongodes atlantica, Palythoa caribeorum, Phyllogorgia dilatata and Plexaurella...
Antimicrobial peptides (AMPs) are an alternative group for the therapy of infectious diseases, with activity against a wide range diverse pathogens. However, classical AMPs have significant side effects in human cells due to their unspecific pore formation biomembranes. Nevertheless, promising therapeutics and can be isolated from natural sources, which include sea freshwater molluscs. The identified these organisms show antimicrobial activities, as pathogens mainly fought by innate defence...