A5090834203- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Venomous Animal Envenomation and Studies
- Amphibian and Reptile Biology
- Essential Oils and Antimicrobial Activity
- Coffee research and impacts
- Aquaculture disease management and microbiota
- Transgenic Plants and Applications
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Aquaculture Nutrition and Growth
- Glycosylation and Glycoproteins Research
- Polydiacetylene-based materials and applications
- Biochemical effects in animals
- Invertebrate Immune Response Mechanisms
- Carbohydrate Chemistry and Synthesis
- Bioactive natural compounds
- Nicotinic Acetylcholine Receptors Study
- Insect Utilization and Effects
- Neuropeptides and Animal Physiology
- Traditional and Medicinal Uses of Annonaceae
- Phytochemistry Medicinal Plant Applications
- Insect and Pesticide Research
- Animal and Plant Science Education
Universidade de Brasília
2015-2025
Brazilian Agricultural Research Corporation
1998-2021
State University of Norte Fluminense
2014
Antimicrobial resistance is a global health threat, which has been worsened by the slow development of new antibiotics. The rational design natural-derived antimicrobial peptides (AMPs) offers promising alternative for enhancing efficacy AMPs and accelerating drug discovery. This paper describes improved peptide derivatives starting from hylin-Pul3, previously isolated frog Boana pulchella, optimizing its hydrophobicity, cationicity, amphipathicity. In silico screening identified six...
Eight new peptides were isolated from the skin secretion of frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing cDNA cloning. Structural similarities between them other antimicrobial genus frogs found. Ocellatins-PT1 to -PT5 (25 residues) are amidated at C-terminus, while ocellatins-PT6 -PT8 (32 have free carboxylates. Antimicrobial activity, hemolytic tests, cytotoxicity against a murine fibroblast cell line investigated. All peptides, except for...
Abstract Cutaneous secretions of amphibians have bioactive compounds, such as peptides, with potential for biotechnological applications. Therefore, this study aimed to determine the primary structure and investigate peptides obtained from cutaneous amphibian, Leptodactylus vastus , a source molecules. The possessed amino acid sequences, GVVDILKGAAKDLAGH GVVDILKGAAKDLAGHLASKV, monoisotopic masses [M + H] ± = 1563.8 Da 2062.4 Da, respectively. molecules were characterized class ocellatins...
Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC de novo sequenced MALDI-TOF MS/MS. Next, we used an silico platform to screen antioxidant molecules framework density functional theory. One identified peptides, salamandrin-I, [M + H]+ = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity...
The anti-inflammatory properties of Turnera subulata have been evaluated as an alternative drug approach to treating several inflammatory processes. Accordingly, in this study, aqueous and hydroalcoholic extracts T. flowers leaves were analyzed regarding their phytocomposition by ultrafast liquid chromatography coupled mass spectrometry, assessed vitro inflammation model, using LPS-stimulated RAW-264.7 macrophages. phytochemical profile indicated vitexin-2-O-rhamnoside important constituent...
Abstract Tryptophyllins constitute a heterogeneous group of peptides that are one the first classes identified from amphibian’s skin secretions. Here, we report structural characterization and antioxidant properties novel tryptophyllin‐like peptide, named PpT‐2, isolated Iberian green frog Pelophylax perezi . The secretion P was obtained by electrical stimulation fractionated using RP‐HPLC. De novo peptide sequencing conducted MALDI MS/MS. primary structure PpT‐2 (FPWLLS‐NH 2 ) confirmed...
Skin secretion of Hypsiboas punctatus is the source a complex mixture bioactive compounds where peptides and small proteins prevail, similarly to many other amphibians. Among dozens molecules isolated from H. in proteomic based approach, we report here structural functional studies novel peptide named Phenylseptin (FFFDTLKNLAGKVIGALT-NH2) that was purified as two naturally occurring D- L-Phes configurations. The amino acid epimerization C-terminal amidation for both were confirmed by...
Colorectal cancer (CRC) ranks second as the leading cause of cancer-related deaths worldwide. N-glycosylation is one most common posttranslational protein modifications. Therefore, we studied total serum N-glycome (TSNG) 13 colon patients compared to healthy controls using MALDI-TOF/MS and LC-MS. tumor samples from same cohort were further quantified a similar methodology. In total, 23 N-glycan compositions down-regulated in patients, mostly galactosylated forms whilst mannose-rich...
Spider venom toxins have raised interest in prospecting new drugs and pesticides. Nevertheless, few studies are conducted with tarantula toxins, especially species found Brazil. This study aims to characterize chemically biologically the first toxin isolated from Acanthoscurria paulensis venom. Ap1a consists of 48 amino acid residues has a molecular mass 5457.79 Da. The cloned gene encodes putative sequence 23 for signal peptide 27 pro-peptide. mature is 60–84% identical those HWTX-II...
The skin glands of amphibian species hold a major component their innate immunity, namely unique set antimicrobial peptides (AMPs). Although most them have common characteristics, differences in AMP sequences allow huge repertoire biological activity with varying degrees efficacy. We present the first study AMPs from Pleurodema somuncurence (Anura: Leptodactylidae: Leiuperinae). Among 11 identified mature peptides, three presented activity. Somuncurin-1 (FIIWPLRYRK), somuncurin-2...
Snake venoms contain various bradykinin-potentiating peptides (BPPs). First studied for their vasorelaxant properties due to angiotensin converting enzyme (ACE) inhibition, these molecules present a range of binding partners, among them the argininosuccinate synthase (AsS) enzyme. This has renewed interest in characterization from biological sources and evaluation pharmacological activities. In work, low molecular weight fraction Bothrops moojeni venom was obtained BPPs were characterized by...
Nematodes are devastating pests that infect most cultivated plant species and cause considerable agricultural losses worldwide. The understanding of metabolic adjustments induced during plant–nematode interaction is crucial to generate resistant plants or select more efficient molecules fight against this pest. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used herein for in situ detection mapping endogenous polypeptides secondary metabolites from...
Type II Congenital Disorders of Glycosylation (CDG-II) are a group diseases with challenging diagnostics characterized by defects in the processing glycans Golgi apparatus. Mass Spectrometry (MS) has been valuable tool definition CDG-II subtypes. While some subtypes associated specific N-glycan structures, others only produce changes relative levels, reinforcing demand for quantification methods.Plasma samples from control individuals were pooled, derivatized deuterated iodomethane (I-CD3),...
In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when shift from water land. We found that Pithecopus azureus secretes single peptide ([M + H]+ = 658.38 Da) at developmental stage precedes onset terrestrial behaviour. De novo and cDNA sequencing revealed peptide, named PaT-2, is expressed in tandem member tryptophyllins family. silico studies allowed us identify position...
Rationale Amphibians can produce a large amount of bioactive peptides over the skin. In order to map precise tissue localization these compounds and evaluate their functions, mass spectrometry imaging (MSI) gene expression studies were used investigate possible correlation between molecules involved in antimicrobial defense mechanisms anti‐predatory behavior by Physalaemus nattereri . Methods Total skin secretion P. was analyzed classical Protein Chemistry proteomic techniques. Intact...