A5039031286- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- Phytase and its Applications
- Studies on Chitinases and Chitosanases
- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Microbial Community Ecology and Physiology
- Plant tissue culture and regeneration
- Pediatric health and respiratory diseases
- Oral microbiology and periodontitis research
- Essential Oils and Antimicrobial Activity
- Antibiotic Resistance in Bacteria
- Phosphorus and nutrient management
- Antifungal resistance and susceptibility
- Biofuel production and bioconversion
- Chromium effects and bioremediation
- Fungal and yeast genetics research
- Transgenic Plants and Applications
- Lichen and fungal ecology
- Plant pathogens and resistance mechanisms
Universidade Católica de Brasília
2017-2025
Brazilian Agricultural Research Corporation
2014-2019
Universidade de Brasília
2015
Universidade Federal de Goiás
2009-2014
The species of T. harzianum are well known for their biocontrol activity against plant pathogens. However, few studies have been conducted to further our understanding its role as a biological control agent S. sclerotiorum, pathogen involved in several crop diseases around the world. In this study, we used RNA-seq and quantitative real-time PCR (RT-qPCR) techniques order explore changes gene expression during growth on cell wall sclerotiorum (SSCW) or glucose. RT-qPCR was also examine genes...
Membrane‐active peptides are useful tools in the design of multifunctional molecules. For example, peptide chimeras may release, after proteolysis membrane‐adsorbed molecules, pharmacologically active fragments. In previous work, Chim2, an antimicrobial composed a membrane‐active module, enzymatic hydrolysis site, and agonist moiety for type 2 formyl receptors (FPR2), was conceptualized. Based on named Chim3 designed, adding consensus sequence bacterial signal peptidase I (Spase I). Spase is...
Antimicrobial resistance is a global health threat, which has been worsened by the slow development of new antibiotics. The rational design natural-derived antimicrobial peptides (AMPs) offers promising alternative for enhancing efficacy AMPs and accelerating drug discovery. This paper describes improved peptide derivatives starting from hylin-Pul3, previously isolated frog Boana pulchella, optimizing its hydrophobicity, cationicity, amphipathicity. In silico screening identified six...
The Trichoderma genus consists of a group free-living filamentous fungi, including species able to act as biological control agents (BCAs) against pathogenic fungi. It is believed that this ability due synergy between several mechanisms, the production wide variety secondary metabolites by these organisms. Among these, we highlight peptaibols, an antibiotic peptide characterized presence non-proteinogenic amino acids such α-aminoisobutyrate (Aib), well N-terminal modifications and alcohols...
Trichoderma harzianum is a fungus well known for its potential as biocontrol agent against many fungal phytopathogens. The aim of this study was to characterize the proteins secreted by T. ALL42 when spores were inoculated and incubated 48 h in culture media supplemented with glucose (GLU) or cell walls from Fusarium solani (FSCW), phytopathogen that causes severe losses common bean soy crops Brazil, other crop diseases around world. able grow Liquid Enzyme Production medium (TLE) Minimal...
HsAFP1, a plant defensin isolated from coral bells (Heuchera sanguinea), is characterized by broad-spectrum antifungal activity. Previous studies indicated that HsAFP1 binds to specific fungal membrane components, which had hitherto not been identified, and induces mitochondrial dysfunction cell permeabilization. In this study, we show reversibly interacts with the phospholipid phosphatidic acid (PA), precursor for biosynthesis of other phospholipids, lesser extent various phosphatidyl...
Examples of bioactive peptides derived from internal sequences proteins are known for decades. The great majority these findings appear to be fortuitous rather than the result a deliberate and methodological-based enterprise. In present work, we describe identification biological activities novel antimicrobial unveiled as fragments various plant founded on our hypothesis-driven search strategy. All putative encrypted were selected based upon their physicochemical properties that iteratively...
Public health problems are associated with device-associated biofilm infections, Candida albicans being the major fungal pathogen. We previously identified potent antibiofilm combination treatment in which antifungal plant defensin HsAFP1 is co-administered caspofungin, preferred antimycotic to treat such infections. In this study, we smallest linear HsAFP1-derived peptide that acts synergistically caspofungin or anidulafungin against C. as HsLin06_18, a 19-mer derived from C-terminal part...
Cationic and amphiphilic peptides can be used as homing devices to accumulate conjugated antibiotics bacteria-enriched sites promote efficient microbial killing. However, just important tackling bacterial infections, is the modulation of immune response in this complex microenvironment. In present report, we designed a peptide chimaera called Chim2, formed by membrane-active module, an enzyme hydrolysis site formyl receptor 2 (FPR2) agonist. This molecule was adsorb onto membranes, their...
Beta-1,3-glucanase is an important cell wall-degrading enzyme involved in mycoparasitism by Trichoderma spp. during antagonism against phytopathogenic fungi. A simple microplate-based method to assay beta-1,3-glucanase activity described here as alternative expensive tube-assay method. The reaction volume of the micro-assay was reduced 130 microl from 1150 used standard macro-assay. Statistical analyses showed significant difference sensitivity between micro- and micro-method optimized using...
Trichoderma asperellum produces two extracellular 1,3-β-d-glucanase upon induction with cell walls from Rhizoctonia solani. A minor was purified to homogeneity by ion exchange chromatography on Q-Sepharose and gel filtration Sephacryl S-100. typical procedure provided 13.8-fold purification 70% yield. SDS–PAGE of the enzyme showed a single protein band molecular weight 27 kDa. The exhibited optimum catalytic activity at pH 3.6 45 °C. It thermostable 40 °C, retained 75% after 60 min Km Vmax...
Abstract Mature proteins can act as potential sources of encrypted bioactive peptides that, once released from their parent proteins, might interact with diverse biomolecular targets. In recent work we introduced a systematic methodology to uncover intragenic antimicrobial (IAPs) within large protein sequence libraries. Given that such may membranes in different ways, resulting distinct observable outcomes, it is desirable develop predictive categorize membrane active and establish link...
Following the treads of our previous works on unveiling bioactive peptides encrypted in plant proteins from diverse species, present manuscript reports occurrence four proof-of-concept intragenic antimicrobial human proteins, named Hs IAPs. These IAPs were prospected using software Kamal, synthesized by solid phase chemistry, and had their interactions with model phospholipid vesicles investigated differential scanning calorimetry circular dichroism. Their activity against bacteria, yeasts...
Acid phosphatases (ACPases) are produced by a variety of fungi and have gained attention due their biotechnological potential in industrial, diagnosis bioremediation processes. These enzymes play specific role scavenging, mobilization acquisition phosphate, enhancing soil fertility plant growth. In this study, new ACPase from Trichoderma harzianum, named II, was purified characterized as glycoprotein belonging to the acid phosphatase family. II presents an optimum pH temperature 3.8 65°C,...
Abstract Bioactive plant peptides have received considerable interest as potential antihypertensive agents with potentially fewer side effects than drugs. Here, the blood pressure-lowering of Bowman-Birk protease inhibitor, BTCI, and its derived peptides, PepChy PepTry, were investigated using normotensive (Wistar-WR) spontaneously hypertensive rats (SHR). BTCI inhibited proteases trypsin chymotrypsin, respectively, at 6 µM 40 µM, a 10-fold greater inhibition observed PepTry (60 µM) (400...