A5063803005- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Amphibian and Reptile Biology
- Protein purification and stability
- Insect Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- vaccines and immunoinformatics approaches
- Advanced Proteomics Techniques and Applications
- Molecular Junctions and Nanostructures
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Neuropeptides and Animal Physiology
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Protein Hydrolysis and Bioactive Peptides
- Glycosylation and Glycoproteins Research
- Genetically Modified Organisms Research
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- Skin and Cellular Biology Research
Centro Científico Tecnológico Patagónico
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2008-2022
Centro Científico Tecnológico - Tucumán
2022
Universidade do Porto
2017
Rede de Química e Tecnologia
2017
University of Buenos Aires
2004-2011
Universitat de Barcelona
2007
Institute for Research in Biomedicine
2007
Antimicrobial resistance is a global health threat, which has been worsened by the slow development of new antibiotics. The rational design natural-derived antimicrobial peptides (AMPs) offers promising alternative for enhancing efficacy AMPs and accelerating drug discovery. This paper describes improved peptide derivatives starting from hylin-Pul3, previously isolated frog Boana pulchella, optimizing its hydrophobicity, cationicity, amphipathicity. In silico screening identified six...
Eight new peptides were isolated from the skin secretion of frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing cDNA cloning. Structural similarities between them other antimicrobial genus frogs found. Ocellatins-PT1 to -PT5 (25 residues) are amidated at C-terminus, while ocellatins-PT6 -PT8 (32 have free carboxylates. Antimicrobial activity, hemolytic tests, cytotoxicity against a murine fibroblast cell line investigated. All peptides, except for...
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions compound beads are first mixed with a target molecule. The that interact this molecule then identified and isolated for structure determination. Here we describe an OBOC peptide library screening using streptavidin (SA) as probe protein, labeled red fluorescent dye the COPAS BIO-BEAD flow sorting equipment separate from nonfluorescent beads. dyes used were ATTO 590 Texas Red. After incubating SA-red...
Small peptides containing fewer than 10 amino acids are promising ligand candidates with which to build affinity chromatographic systems for industrial protein purification. The application of combinatorial peptide synthesis strategies greatly facilitates the discovery suitable ligands any given interest. Here we sought identify recombinant human erythropoietin (rhEPO), is used treatment anemia. A library octapeptides X-X-X-Phe-X-X-Ala-Gly, where X = Ala, Asp, Glu, Phe, His, Leu, Asn, Pro,...
The potential emergence of zoonotic diseases has raised significant concerns, particularly in light the recent pandemic, emphasizing urgent need for scientific preparedness. bioprospection and characterization new molecules are strategically relevant to research development innovative drugs viral bacterial treatment disease management. Amphibian species possess a diverse array compounds, including antimicrobial peptides. This study identified first bioactive peptide from Salamandra...
Although the mechanism of action antimicrobial peptides (AMPs) is not clear, they can interact electrostatically with cell membranes microorganisms. New ocellatin-PT were recently isolated from skin secretion Leptodactylus pustulatus. The secondary structure these AMPs and their effect on Leishmania infantum cells, different lipid surface models was characterized in this work. results showed that all have an α-helix five them (PT3, PT4, PT6 to PT8) leishmanicidal activity; PT1 PT2 affected...
Peptides from skin secretions of amphibians are considered important components their immune system and also play a relevant role in defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence 13 peptides gland secretion hylid tree frog, Boana punctata. Using situ matrix-assisted laser desorption ionization imaging MS transverse section tissue, show that some stored as longer molecules cleaved after being secreted, whereas others do not undergo...
Amphibians’ skin is a rich source of natural antimicrobial peptides (AMPs). These AMPs exhibit marked inter- and intraspecific sequence divergence linked to the arms race between host pathogens. Here, we combine peptidomics, molecular modeling, phylogenetic analyses understand evolution in Cophomantini, diverse clade neotropical tree frogs, investigate their interaction with bacterial membranes. Consistent results other amphibians, all species Cophomantini secrete mixture peptides. We...
The skin glands of amphibian species hold a major component their innate immunity, namely unique set antimicrobial peptides (AMPs). Although most them have common characteristics, differences in AMP sequences allow huge repertoire biological activity with varying degrees efficacy. We present the first study AMPs from Pleurodema somuncurence (Anura: Leptodactylidae: Leiuperinae). Among 11 identified mature peptides, three presented activity. Somuncurin-1 (FIIWPLRYRK), somuncurin-2...
For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation referred was performed. The main objective this study selection set peptides that could function potential immunogens for production novel antibodies against CP4-EPSPS protein. purpose, cleaved with trypsin/chymotrypsin and resultant were extensively analyzed further best candidates antibody production....
The one-bead-one-compound method gives access to millions of compounds that can be screened directly on the bead. Although characterization techniques are increasingly potent and reliable, problems still encountered in deciphering sequence active compound because sensitiveness or manipulation ChemMatrix, a totally PEG-based resin, has resolved synthesis peptides outstanding difficulty. Like other resins, it permits on-bead screening its compatibility aqueous media further advantage having...