A5045761284- Antimicrobial Peptides and Activities
- Essential Oils and Antimicrobial Activity
- Advanced biosensing and bioanalysis techniques
- Biochemical and Structural Characterization
- Nanoparticles: synthesis and applications
- Insect Resistance and Genetics
- Psidium guajava Extracts and Applications
- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Ginkgo biloba and Cashew Applications
- Molecular Junctions and Nanostructures
- Biosensors and Analytical Detection
- Protein Hydrolysis and Bioactive Peptides
- Advanced Chemical Sensor Technologies
- Inflammatory mediators and NSAID effects
- Phytochemicals and Medicinal Plants
- Pesticide Residue Analysis and Safety
- Polydiacetylene-based materials and applications
- Antioxidant Activity and Oxidative Stress
- Photodynamic Therapy Research Studies
- Analytical chemistry methods development
- Neuropeptides and Animal Physiology
- Bioactive natural compounds
- Phytochemicals and Antioxidant Activities
- Amphibian and Reptile Biology
Rede de Química e Tecnologia
2016-2025
Universidade do Porto
2012-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2019-2020
Polytechnic Institute of Porto
2012-2020
Instituto de Biologia Molecular e Celular
2019-2020
Instituto Superior de Engenharia do Porto
2012
Silver nanoparticles have been shown to possess considerable antibacterial activity, but in vivo applications limited due the inherent, low, toxicity of silver. On other hand, silver could provide cutaneous protection against infection, their ability liberate ions via a slow release mechanism, and broad-spectrum antimicrobial action. Thus, this work, we describe development carboxymethyl cellulose-based hydrogel containing nanoparticles. The were prepared situ, utilizing two variants cashew...
Silver nanoparticles have been studied as an alternative for treatment of microbial infections and leishmaniasis, without promoting induction or parasite resistance. In this study, chitosan-based silver were synthesized from nitrate (AgNO 3 ), sodium borohydride a reducing agent, the biopolymer chitosan capping agent. The characterized by ultraviolet–visible, Fourier transform infrared, dynamic light scattering, zeta potential, atomic force microscopy, transmission electron microscope....
Aim: To test ocellatin peptides (ocellatins-PT2–PT6) for antibacterial and antibiofilm activities synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays microscopic analysis by confocal laser scanning microscopy atomic force microscopy. Results: Ocellatins more active multidrug-resistant isolates of P. aeruginosa than susceptible strains. Ocellatin-PT3...
The aims of this study were to produce poly-ɛ-caprolactone lipid-core nanocapsules containing lycopene-rich extract from red guava (LEG), characterize those nanoparticles and evaluate their cytotoxic effects on human breast cancer cells. Lipid-core the (nanoLEG) produced by method interfacial deposition preformed polymer. characterized Dynamic Light Scattering (DLS), Polydispersity Index, Zeta Potential, pH, Encapsulation Efficiency, Nanoparticle Tracking Analysis (NTA), Atomic Force...
Abstract Cutaneous secretions of amphibians have bioactive compounds, such as peptides, with potential for biotechnological applications. Therefore, this study aimed to determine the primary structure and investigate peptides obtained from cutaneous amphibian, Leptodactylus vastus , a source molecules. The possessed amino acid sequences, GVVDILKGAAKDLAGH GVVDILKGAAKDLAGHLASKV, monoisotopic masses [M + H] ± = 1563.8 Da 2062.4 Da, respectively. molecules were characterized class ocellatins...
Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC de novo sequenced MALDI-TOF MS/MS. Next, we used an silico platform to screen antioxidant molecules framework density functional theory. One identified peptides, salamandrin-I, [M + H]+ = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity...
Snake venoms are important sources of bioactive molecules, including those with antiparasitic activity. Cathelicidins form a class such which produced by variety organisms. Batroxicidin (BatxC) is cathelicidin found in the venom common lancehead (
Abstract Tryptophyllins constitute a heterogeneous group of peptides that are one the first classes identified from amphibian’s skin secretions. Here, we report structural characterization and antioxidant properties novel tryptophyllin‐like peptide, named PpT‐2, isolated Iberian green frog Pelophylax perezi . The secretion P was obtained by electrical stimulation fractionated using RP‐HPLC. De novo peptide sequencing conducted MALDI MS/MS. primary structure PpT‐2 (FPWLLS‐NH 2 ) confirmed...
Although the mechanism of action antimicrobial peptides (AMPs) is not clear, they can interact electrostatically with cell membranes microorganisms. New ocellatin-PT were recently isolated from skin secretion Leptodactylus pustulatus. The secondary structure these AMPs and their effect on Leishmania infantum cells, different lipid surface models was characterized in this work. results showed that all have an α-helix five them (PT3, PT4, PT6 to PT8) leishmanicidal activity; PT1 PT2 affected...
The potential emergence of zoonotic diseases has raised significant concerns, particularly in light the recent pandemic, emphasizing urgent need for scientific preparedness. bioprospection and characterization new molecules are strategically relevant to research development innovative drugs viral bacterial treatment disease management. Amphibian species possess a diverse array compounds, including antimicrobial peptides. This study identified first bioactive peptide from Salamandra...
Copper nanoparticles stabilized with cashew (CG-CuNPs) were synthesized by reduction reaction using ascorbic acid and sodium borohydride, the gum (CG) as a natural polymer stabilizer. Dynamic light scattering, atomic force microscopy, Fourier-transform infrared spectroscopy, UV-Vis spectrophotometry, x-ray diffraction used to characterize (CG-CuNPs), copper was quantified electrochemical measurement. The UV-vis spectra of CG-CuNPs confirmed formation appearance surface plasmon band at 580 nm...