A5091861193- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- vaccines and immunoinformatics approaches
- Synthesis and Biological Evaluation
- Parasites and Host Interactions
- Genomics and Phylogenetic Studies
- Toxin Mechanisms and Immunotoxins
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Synthesis and biological activity
- Vector-Borne Animal Diseases
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Toxoplasma gondii Research Studies
- Machine Learning in Bioinformatics
- Bacterial Identification and Susceptibility Testing
- Iron Metabolism and Disorders
- Diphtheria, Corynebacterium, and Tetanus
- Vector-borne infectious diseases
- Bioactive Compounds and Antitumor Agents
- Signaling Pathways in Disease
- Neutropenia and Cancer Infections
- Vibrio bacteria research studies
- T-cell and Retrovirus Studies
- Acute Lymphoblastic Leukemia research
Fundação Oswaldo Cruz
2014-2025
Universidade Federal de Ouro Preto
2011-2014
Companhia Energética de Minas Gerais (Brazil)
2014
Universidade Federal de Minas Gerais
2008
Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such goats and sheep; it also causes infections in humans, though rarely. This species distributed worldwide, but has most serious economic impact Oceania, Africa South America. Although C. pseudotuberculosis major health productivity problems for livestock, little about molecular basis...
Polymerase chain reaction (PCR) and its variations represent highly sensitive specific methods for Leishmania DNA detection subsequent canine visceral leishmaniasis (CVL) diagnosis. The aim of this work was to compare three different molecular diagnosis techniques (conventional PCR [cPCR], seminested [snPCR], quantitative [qPCR]) in samples skin spleen from 60 seropositive dogs by immunofluorescence antibody test enzyme-linked immunosorbent assay. Parasitological analysis conducted culture...
Chagas disease (CD) is a neglected tropical caused by Trypanosoma cruzi. The current drugs used to treat these diseases have limited efficacy and produce severe side effects. 4-aminoquinoline derivatives were shown be promising class of inhibitors cysteine proteases cruzain TbrCATL. To evaluate the trypanocidal activity new series aminoquinolines as potential Three synthesised their in vitro was evaluated against TbrCATL well amastigotes trypomastigotes forms T. In silico studies also...
Many vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them efficacious for the human or canine disease. On this basis, design vaccines has constantly changing, and use approaches select specific epitopes seems be crucial in scenario. The ability predict T cell-specific makes immunoinformatics an even more necessary approach, as VL efficient immune response parasite is triggered by lymphocytes Leishmania spp. immunogenic antigens. Moreover,...
The Trypanosomatids parasites Leishmania braziliensis, major and infantum are important human pathogens. Despite of years study genome availability, effective vaccine has not been developed yet, the chemotherapy is highly toxic. Therefore, it clear just interdisciplinary integrated studies will have success in trying to search new targets for developing vaccines drugs. An essential part this rationale related protein-protein interaction network (PPI) which can provide a better understanding...
Desulfovibrio gigas is a model organism of sulfate-reducing bacteria which energy metabolism and stress response have been extensively studied. The complete genomic context this was however, not yet available. sequencing the D. genome provides insights into integrated network conserving complexes structures present in bacterium. Comparison with genomes other spp. reveals presence two different CRISPR/Cas systems gigas. Phylogenetic analysis using conserved protein sequences (encoded by rpoB...
Abstract Background One of the major challenges to leishmaniasis treatment is emergence parasites resistant antimony. To study differentially expressed genes associated with drug resistance, we performed a comparative transcriptomic analysis between wild-type and potassium antimonyl tartrate (Sb III )-resistant Leishmania infantum lines using high-throughput RNA sequencing. Methods All cDNA libraries were constructed from promastigote forms each line, sequenced analyzed STAR for mapping...
Diagnosing canine visceral leishmaniasis (CVL) in Brazil faces challenges due to the limitations regarding sensitivity and specificity of current diagnostic protocol. Therefore, it is urgent map new antigens or enhance existing ones for future techniques. Immunoinformatic tools are promising identification potential epitopes antigen candidates. In this study, we evaluated peptides selected by epitope prediction CVL serodiagnosis ELISA assays. Ten B-cell were immunogenic silico, but two...
Abstract Background Epitope prediction using computational methods represents one of the most promising approaches to vaccine development. Reduction time, cost, and availability completely sequenced genomes are key points highly motivating regarding use reverse vaccinology. Parasites genus Leishmania widely spread they etiologic agents leishmaniasis. Currently, there is no efficient against this pathogen drug treatment toxic. The lack sufficiently large datasets experimentally validated...
Leishmaniasis is a wide-spectrum disease caused by parasites from Leishmania genus. There no human vaccine available and it considered many studies as apotential effective tool for control. To discover novel antigens, computational programs have been used in reverse vaccinology strategies. In this work, we developed validation antigen approach that integrates prediction of B T cell epitopes, analysis Protein-Protein Interaction (PPI) networks metabolic pathways. We selected twenty candidate...
Reverse vaccinology or immunoinformatics is a computational methodology which integrates data from in silico epitope prediction, associated to other important information as, for example, the predicted subcellular location of proteins used design context vaccine development. This approach has potential search new targets development proteome pathogenic organisms. To date, there no effective employed vaccination campaigns against visceral leishmaniasis (VL). For first time, herein, an silico,...
In order to effectively control and monitor schistosomiasis, new diagnostic methods are essential. Taking advantage of computational approaches provided by immunoinformatics considering the availability Schistosoma mansoni predicted proteome information, candidate antigens schistosomiasis were selected used in immunodiagnosis tests based on Enzime-linked Immunosorbent Assay (ELISA). The selection strategy was signal peptide prediction; low similarity human proteins; B- T-cell epitope...
Abstract Chagas disease is a neglected tropical disease, endemic in Latin America and caused by the protozoan parasite Trypanosoma cruzi . Available treatments show low cure efficacy during chronic phase of cause series side effects, reinforcing need to develop new drugs against disease. In this work, we describe optimization trypanocidal hit compound recently reported phenotypic high‐throughput screening studies A hit‐to‐lead process was initiated structure‐activity relationship obtained...
Chagas disease, caused by the protozoan Trypanosoma cruzi , is a serious chronic parasitic currently treated with Nifurtimox (NFX) and Benznidazole (BZ). In addition to high toxicity, these drugs have low healing efficacy, especially in phase of disease. The existence drug-resistant T . strains occurrence cross-resistance between BZ NFX also been described. this context, it urgent study metabolism better understand mechanisms resistance. Prostaglandin F2α synthase (PGFS) an enzyme that has...
Campylobacter fetus subsp. venerealis is the etiologic agent of bovine genital campylobacteriosis, a sexually transmitted disease cattle that worldwide importance. The complete sequencing and annotation genome type strain C. NCTC 10354(T) are reported.
Malaria, Chagas disease, and leishmaniasis are tropical diseases caused by protozoan parasites of the genera Plasmodium, Trypanosoma Leishmania, respectively. These constitute a major burden on public health in several regions worldwide, mainly affecting low-income populations economically poor countries. Severe side effects currently available drug treatments emergence resistant need to be addressed development novel candidates. Natural 2,5-Diketopiperazines (2,5-DKPs) N-heterocyclic...
Triatoma infestans is an insect of subfamily Triatominae (Hemiptera: Reduviidae) and important vector Trypanosoma cruzi, the etiologic agent human Chagas disease. In this work we reported a transcriptome assembly annotation T. heads obtained by Next Generation Sequencing (NGS) technologies.