A5028510255- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Biochemical and Molecular Research
- Cellular transport and secretion
- Thyroid Disorders and Treatments
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Neuroscience and Neuropharmacology Research
- Cardiomyopathy and Myosin Studies
- Synthesis and Biological Evaluation
- Adenosine and Purinergic Signaling
- Genetic Neurodegenerative Diseases
- Glycosylation and Glycoproteins Research
- Genetics, Bioinformatics, and Biomedical Research
- Neurobiology and Insect Physiology Research
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Nuclear Structure and Function
- Lysosomal Storage Disorders Research
- RNA Research and Splicing
- Redox biology and oxidative stress
- Congenital heart defects research
- Extracellular vesicles in disease
Universidade de São Paulo
2009-2024
Clinics Hospital of Ribeirão Preto
2016
Universidade de Ribeirão Preto
2008-2014
Harvard University
2008
Hospital Universitario Santa Cristina
2008
Brigham and Women's Hospital
2003
Institut National de Recherche et de Sécurité
2001
Harvard University Press
2000
The type 3 iodothyronine selenodeiodinase (D3) is an integral membrane protein that inactivates thyroid hormones. By using immunofluorescence cytochemistry confocal microscopy of live or fixed cells transiently expressing FLAG-tagged human D3 monkey hepatocarcinoma endogenous D3, we identified in the plasma membrane. It co-localizes with Na,K-ATPase alpha, early endosomal marker EEA-1 and clathrin, but not two endoplasmic reticulum resident proteins. Most molecule extracellular can be...
We compared the subcellular localization of FLAG-epitope tagged Types 1 and 2 deiodinases (D1 D2) transiently expressed in human embryonic kidney (HEK-293) mouse neuroblastoma (NB2A) cells. D2 is an integral membrane protein based on resistance to extraction at pH 11 with NH2 terminus endoplasmic reticulum (ER). Immunofluorescence confocal microscopy using anti-FLAG anti-GRP78/BiP antibodies showed FLAG-D1 signal was found periphery cells not co-localized ER specific marker GRP78/BiP. On...
Types 1 and 3 iodothyronine deiodinases are known to be selenocysteine-containing enzymes. Although a putative human type 2 deiodinase (D2) gene (hDio2) encoding similar selenoprotein has been identified, basal D2 activity is not selenium (Se)-dependent nor labeled with75Se. A mesothelioma cell line (MSTO-211H) recently shown have ∼40-fold higher levels ofhDio2 mRNA than mesothelial cells. Mesothelioma lysates activate thyroxine (T4) 3,5,3′-triiodothyronine with typical characteristics of...
Abstract Nuclear actin and nuclear myosins have been implicated in the regulation of gene expression vertebrate cells. Myosin V is a class actin‐based motor proteins involved cytoplasmic vesicle transport anchorage, spindle‐pole alignment mRNA translocation. In this study, myosin‐Va, phosphorylated on conserved serine tail domain (phospho‐ser 1650 MVa), was localized to subnuclear compartments. A monoclonal antibody, 9E6, raised against peptide corresponding phosphoserine flanking regions...
Kinases can recognize consensus motifs that are noncontiguous in folded proteins.
In the past years, extracellular vesicles (EVs) have become an important field of research since EVs been found to play a central role in biological processes. pathogens, are involved several events during host-pathogen interaction, including invasion, immunomodulation, and pathology as well parasite-parasite communication. this report, we summarised infections caused by viruses, bacteria, fungi, protozoa, helminths based on talks discussions carried out International Society for...
Background Visceral leishmaniasis is an important tropical disease, and Leishmania infantum chagasi (synonym of infantum) the main pathogenic agent visceral in New World. Recently, ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases) were identified as enablers infection virulence factors many pathogens. Two putative E-NTPDases (∼70 kDa ∼45 kDa) have been found L. genome. Here, we studied E-NTPDase from to describe its natural occurrence, biochemical characteristics influence on...
ABSTRACT The flagellum and attachment zone (FAZ) are important cytoskeletal structures in trypanosomatids, being required for motility, cell division morphogenesis. Trypanosomatid cytoskeletons contain abundant high molecular mass proteins (HMMPs), but many of their biological functions still unclear. Here, we report the characterization giant FAZ protein, FAZ10, Trypanosoma brucei, which, using immunoelectron microscopy, show localizes to intermembrane staples intracellular domain. Our data...
We compared the subcellular localization of FLAG-epitope tagged Types 1 and 2 deiodinases (D1 D2) transiently expressed in human embryonic kidney (HEK-293) mouse neuroblastoma (NB2A) cells. D2 is an integral membrane protein based on resistance to extraction at pH 11 with NH2 terminus endoplasmic reticulum (ER). Immunofluorescence confocal microscopy using anti-FLAG anti-GRP78/BiP antibodies showed FLAG-D1 signal was found periphery cells not co-localized ER specific marker GRP78/BiP. On...
Previous work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 evaluated its nucleotidase activity, cellular localization role parasite adhesion to host cells. was able utilize broad range triphosphate diphosphate nucleosides. The enzyme's Km ATP (0.096 mM) capability influence host's ATP-dependent purinergic...
We report the production and availability of over 7000 fully sequence verified plasmid ORF clones representing 3400 unique human genes. These were derived using MGC collection as template produced in two formats: with without stop codons. Thus, this supports either native protein or proteins fusion tags added to both ends. The used generate enriched three ways. First, gene redundancy was removed. Second, selected represent best available GenBank reference sequence. Finally, a...
Skp1, Cul1, Rbx1, and the FBXO25 protein form a functional ubiquitin ligase complex. Here, we investigate cellular distribution of its colocalization with some nuclear proteins by using immunochemical biochemical approaches. was monitored affinity-purified antibodies raised against recombinant fragment spanning residues 2-62 sequence. expressed in all mouse tissues tested except striated muscle, as indicated immunoblot analysis. Confocal analysis revealed that endogenous partially...
FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family ubiquitin ligases composed s-phase-kinase associated protein 1, really interesting new gene-box Cullin and (SCF1) are involved in targeting destruction across proteasome system. We recently reported accumulates novel subnuclear structures named FBXO25-associated nuclear domains (FAND). Combining two-step affinity purification followed by MS with classical two-hybrid screen, we identified 132 potential...
Host manipulation is a common strategy for invading pathogens. Trypanosoma cruzi, the causative agent of Chagas Disease, lives intracellularly within host cells. During infection, parasite-associated modifications occur to cell metabolism and morphology. However, little known about effect T. cruzi infection on nucleus nuclear functionality. Here, we show that can modulate transcription splicing machinery in non-professional phagocytic cells during infection. We found regulates RNA polymerase...
Drebrins are crucial for synaptic function and dendritic spine development, remodeling, maintenance. In temporal lobe epilepsy (TLE) patients, a significant hippocampal reorganization occurs, has been associated with hyperexcitability. This study aimed to evaluate, in TLE the expression of drebrin using immunohistochemistry DAS2 or M2F6 antibodies that recognize adult (drebrin A) embryonic (pan-drebrin) isoforms, respectively.Hippocampal sections from drug-resistant patients sclerosis (HS;...
Promastigote forms of Phytomonas serpens, Leptomonas samueli, and Leishmania tarentolae express cytoskeletal giant proteins with apparent molecular masses 3,500 kDa (Ps 3500), 2,500 (Ls 2500), 1,200 (Lt 1200), respectively. Polyclonal antibodies to Lt 1200 Ps 3500 specifically recognize similar polypeptides the same genera parasite. In addition reacting species, anti-Ls 2500 also cross reacts 3500, a 500-kDa polypeptide Leishmania. Confocal immunofluorescence immunogold electron microscopy...
Trypanosoma cruzi, the etiological agent of Chagas disease, invades many cell types affecting numerous host-signalling pathways. During T. cruzi infection, we demonstrated modulations in host RNA polymerase II activity with downregulation ribonucleoproteins transcription and splicing machinery. These alterations could be a result initial damage to DNA caused by presence parasite, however, mechanisms are not well understood. Herein, examined whether infection coincided enhanced cell. We...