A5052151643- Neuropeptides and Animal Physiology
- Cell Adhesion Molecules Research
- Renin-Angiotensin System Studies
- Nitric Oxide and Endothelin Effects
- Peptidase Inhibition and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroscience and Neuropharmacology Research
- Asthma and respiratory diseases
- Protein Hydrolysis and Bioactive Peptides
- Cellular transport and secretion
- Epilepsy research and treatment
- Cardiomyopathy and Myosin Studies
- Immune Response and Inflammation
- Muscle Physiology and Disorders
- Cerebrovascular and Carotid Artery Diseases
- Chemokine receptors and signaling
- Glycosylation and Glycoproteins Research
- Neuroendocrine regulation and behavior
- Neonatal Respiratory Health Research
- Protease and Inhibitor Mechanisms
- Receptor Mechanisms and Signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fetal and Pediatric Neurological Disorders
- Pain Mechanisms and Treatments
- Nuclear Receptors and Signaling
Yale University
2024
Hospital Prof. Dr. Fernando Fonseca
2007-2023
Universidade de São Paulo
2004-2020
Stockholm University
2018-2019
Instituto de Neurociências e Comportamento
2018
Universidade de Ribeirão Preto
1989-2013
Universidade Federal do Triângulo Mineiro
2011-2013
Clinics Hospital of Ribeirão Preto
2003
Universidade Estadual de Campinas (UNICAMP)
1997
Two thiol-activated endopeptidases with pH optima near 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively ion-exchange chromatography an amino acid analyzer. Brain kininase A hydrolyzes Phe5-Ser6 peptide in bradykinin (Bk), Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9. It is isoelectric 5.2 has a molecular weight approximately 71 000. The...
Our hypothesis is that oxytocin (OT) causes natriuresis by activation of renal NO synthase releases followed cGMP mediates the natriuresis. To test this hypothesis, an inhibitor synthase, l -nitroarginine methyl ester (NAME), was injected into male rats. Blockade release NAME had no effect on induced atrial natriuretic peptide (ANP). This presumably caused because ANP also activates guanylyl cyclase, which synthesizes from GTP. The 18-fold increase in sodium (Na + ) excretion OT (1 μg)...
To establish the correlation between quantitative analysis based on B-mode ultrasound images of vulnerable carotid plaque and histological examination surgically removed plaque, basis a videodensitometric digital texture characterization. Twenty-five patients (18 males, mean age 67 ± 6.9 years) admitted for endarterectomy extracranial high-grade internal artery stenosis (≥ 70% luminal narrowing) underwent to ultrasonic tissue characterization before surgery. A computer software (Carotid...
Eosinophil migration in vivo is markedly attenuated rats treated chronically with the NO synthase (NOS) inhibitor N ω-nitro- l -arginine methyl ester ( -NAME). In this study, we investigated existence of a NOS system eosinophils. Our results demonstrated that rat peritoneal eosinophils strongly express both type II (30.2 ± 11.6% counted cells) and III (24.7 7.4% NOS, as detected by immunohistochemistry using affinity purified mouse mAbs. vitro was evaluated 48-well microchemotaxis chambers...
Expression of nitric oxide synthase isoforms and nitrotyrosine immunoreactivity by B‐cell non‐Hodgkin’s lymphomas multiple myeloma Aims : Nitric synthases (NOS) are isoenzymes that catalyse the synthesis (NO). The three main NOS are: NOS1 or neuronal, NOS2 inducible, NOS3 endothelial. NO plays both physiological pathological roles, depending on its rate concentration, cellular source microenvironment. Apoptosis is an important biological factor in low‐grade lymphomas, able to prevent...
The Panayiotopoulos type of occipital lobe epilepsy has generated great interest, but the particular brain areas involved in peculiar seizure manifestations have not been established. We studied a patient with syndrome, using high-resolution EEG and simultaneous functional magnetic resonance imaging (fMRI). Resolution scalp was improved realistic spline Laplacian algorithm, produced complex distribution current sinks sources over lobe. spike-related blood oxygen level dependent (BOLD) effect...
There is accumulating evidence that autonomic dysfunction occurs in HIV infection. While many studies have demonstrated abnormalities on clinical basis, only one has studied the morphology of sympathetic ganglia. The superior ganglia 12 randomly selected AIDS patients and those 6 controls were examined morphologically order to determine frequency severity their involvement. Although they had not been investigated for dysfunction, 5 suffered from non-infectious diarrhoea, showed bilateral...
Nitric oxide (NO) has been implicated in a variety of functions, including the control synaptic plasticity and sensory signaling. Current evidence suggests that this unconventional neurotransmitter mediates N-methyl-d-aspartate (NMDA) receptor-linked excitotoxicity. This study describes expression neuronal NO synthase (nNOS) immunoreactivity (IR) hippocampi from patients with temporal lobe epilepsy (TLE).Hippocampi clinical symptoms, neuroimaging, EEG typical hippocampal sclerosis (HS; n =...
The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and steam cooker.Myosin-V, a molecular motor involved in vesicle transport, was as neuronal marker honeybee Apis mellifera brains fixed formalin.Overall, cooker showed most satisfactory AR results.At 100 o C, tissue morphology maintained revealed epitope recovery, while evaporation of solution markedly reduced; this is important stabilizing sodium citrate molarity...
To analyse histological composition and progression of carotid plaque.Thirty-one patients (22 males, mean age 68.03 +/- 7.3 years) admitted for endarterectomy extracranial high-grade internal artery stenosis (>or= 70% luminal narrowing) were enrolled. The divided into 2 groups according to symptomatology (group I, 17 symptomatic patients; group II, 14 asymptomatic patients). A analysis inflammatory cell quantification each excised plaque was made. Nine arteries removed from human cadavers...