A5065170627- Animal Virus Infections Studies
- Virus-based gene therapy research
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Synthesis and biological activity
- RNA modifications and cancer
- Synthesis and Characterization of Heterocyclic Compounds
- Adenosine and Purinergic Signaling
- Herpesvirus Infections and Treatments
- Cancer-related gene regulation
- Phytochemicals and Medicinal Plants
- Microbial infections and disease research
- Phytochemical compounds biological activities
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Essential Oils and Antimicrobial Activity
- Proteins in Food Systems
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Aquaculture disease management and microbiota
Universidade Federal de Viçosa
2016-2025
Cornell University
2021
Universidade Unigranrio
2021
Universidade Estadual de Campinas (UNICAMP)
2006-2014
Brazilian Center for Research in Energy and Materials
2010-2014
Brazilian Biosciences National Laboratory
2014
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2010
Brazilian Synchrotron Light Laboratory
2006-2009
Universidade de São Paulo
2008
Istituto Zooprofilattico Sperimentale delle Venezie
1990
Dysregulation of pre-mRNA splicing machinery activity has been related to the biogenesis several diseases. The serine/arginine-rich protein kinase family (SRPKs) plays a critical role in regulating events through extensive phosphorylation factors from proteins (SR proteins). Previous investigations have described overexpression SRPK1 and SRPK2 leukemia other cancer types, suggesting that they would be useful targets for developing novel antitumor strategies. Herein, we evaluated effect...
Melanoma is the most aggressive skin cancer, and its incidence has continued to rise during past decades. Conventional treatments present severe side effects in cancer patients, melanoma can be refractory commonly used anticancer drugs, which justify efforts find new potential anti-melanoma drugs. An alternative promote discovery of pharmacological substances would modifying chemical groups from a bioactive compound. Here we describe synthesis seventeen compounds derived cinnamic acid their...
Identifying binding sites is crucial for expanding our knowledge of various biological processes, supporting drug discovery, and repositioning strategies, particularly in the early research phases target hopping. This especially important neglected diseases, which predominantly impact vulnerable populations developing regions. To address this, we created BENDER DB, a database designed to map predicted protein within proteomes pathogens associated with diseases. Utilizing AlphaFold-predicted...
Porcine circovirus 3 (PCV3) is an emerging virus that was first identified in the United States 2016. Since its detection, PCV3 has already been found America, Asia, and Europe. Although described Brazil, knowledge of detection sequence variation before 2016 limited, as well distribution main swine producing regions Brazil. In this study, 67 porcine clinical samples collected from nine states Brazil between 2006 2007 were analyzed for infection by PCR. Results showed 47.8% positive, across...
Three porcine circovirus-2 strains were isolated from pigs on a Brazilian farm during an outbreak, indicating vaccine failure. They present identical genomic sequences, with high identities to other isolates that also related vaccination failures, supporting the recent theory about antigen drift being associated failures throughout world.
Abstract The fasciculation and elongation protein Zeta 1 (FEZ1) is the mammalian orthologue of Caenorhabditis elegans UNC‐76, which necessary for axon growth. Human FEZ1 interacts with Protein Kinase C (PKC) several regulatory proteins involved in functions ranging from microtubule associated transport to transcriptional regulation. Theoretical prediction, circular dichroism, fluorescence spectroscopy, limited proteolysis recombinant suggest that it contains disordered regions, especially...
A series of thirteen C-3 functionalized isobenzofuran-1(3H)-ones (phtalides) was synthesized via condensation, aromatization, and acetylation reactions. NMR (one two dimensional experiments), IR, mass spectrometry analysis allowed confirmation the identity compounds. The substances were submitted to in vitro bioassays against U937 (lymphoma) K562 (myeloid leukemia) cancer cell lines using MTT cytotoxicity assay. Some derivatives inhibited 90% viability at 100 µM. Also, phtalides presented...
Background Visceral leishmaniasis is an important tropical disease, and Leishmania infantum chagasi (synonym of infantum) the main pathogenic agent visceral in New World. Recently, ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases) were identified as enablers infection virulence factors many pathogens. Two putative E-NTPDases (∼70 kDa ∼45 kDa) have been found L. genome. Here, we studied E-NTPDase from to describe its natural occurrence, biochemical characteristics influence on...
The study of protein interactions has generated great interest in the food industry. Therefore, research on new supramolecular structures shows promise. Supramolecular whey proteins α-lactalbumin and glycomacropeptide were produced under varying heat treatments (25 to 75°C) acidic conditions (pH3.5 6.5). Isothermal titration calorimetry experiments showed demonstrated that this is an enthalpically driven process. aqueous solutions characterized by circular dichroism intrinsic fluorescence...
The human 57 kDa Ki‐1 antigen (Ki‐1/57) is a cytoplasmic and nuclear protein, associated with Ser/Thr protein kinase activity, phosphorylated at the serine threonine residues upon cellular activation. We have shown that Ki‐1/57 interacts chromo‐helicase DNA‐binding domain 3 adaptor/signaling receptor of activated 1 in nucleus. Among identified proteins interacted yeast two‐hybrid system was arginine‐methyltransferase‐1 (PRMT1). Most interestingly, when PRMT1 used as bait we were able to...
The Serine/arginine-rich protein kinases (SRPK) are involved in pre-mRNA splicing control through the phosphorylation of SR family factors. Over last years, several studies have shown relevance SRPK for human cancers and their potential as promising drug targets. In this context, we previously selected three trifluoromethyl arylamides (named here SRVIC24, SRVIC30 SRVIC36) with improved vitro antileukemia effect ability impairing cellular activity SRPK. Given increasing amount reports on...
Previous work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 evaluated its nucleotidase activity, cellular localization role parasite adhesion to host cells. was able utilize broad range triphosphate diphosphate nucleosides. The enzyme's Km ATP (0.096 mM) capability influence host's ATP-dependent purinergic...
The human protein Ki-1/57 was first identified through the cross reactivity of anti-CD30 monoclonal antibody Ki-1, in Hodgkin lymphoma cells. expression diverse cancer cells and its phosphorylation peripheral blood leukocytes after mitogenic activation suggested possible role cell signaling. interacts with several other regulatory proteins involved cellular signaling, transcriptional regulation RNA metabolism, suggesting it may have pleiotropic functions. In a previous spectroscopic...
Abstract The human SFRS9/SRp30c belongs to the SR family of splicing regulators. Despite evidence that members this protein may be targeted by arginine methylation, has yet experimentally addressed. In study, we found SFRS9 is a target for PRMT1-mediated methylation in vitro, and it immunoprecipitated from HEK-293 lysates antibodies recognize both mono- dimethylated arginines. We further observed upon treatment with inhibitor Adox, fluorescent EGFP-SFRS9 re-localizes dot-like structures cell...
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression fluorescent protein GFP by Leishmania braziliensis METHODS L. braziliensis-GFP was generated using tarentolae pLEXSY-egfp GFP. Fluorescent were selected and subjected to standardisation tests anti-promastigote anti-intracellular amastigote assays. FINDINGS Our results showed that is faster more sensitive than Allamar Blue-resazurin. MAIN...