A5014412302- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Neurogenetic and Muscular Disorders Research
- Plant Virus Research Studies
- Nuclear Structure and Function
- Protein Structure and Dynamics
- Amyotrophic Lateral Sclerosis Research
- Developmental Biology and Gene Regulation
- Signaling Pathways in Disease
- Plant Pathogenic Bacteria Studies
- Microtubule and mitosis dynamics
- Enzyme Structure and Function
- Plant-Microbe Interactions and Immunity
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Wnt/β-catenin signaling in development and cancer
- Viral Infections and Immunology Research
University of Helsinki
2015-2019
University of Massachusetts Chan Medical School
2010-2015
Brazilian Center for Research in Energy and Materials
2012
Brazilian Biosciences National Laboratory
2012
Brazilian Synchrotron Light Laboratory
2006-2009
Universidade Estadual de Campinas (UNICAMP)
2006-2008
Amyotrophic lateral sclerosis (ALS)-linked fused in sarcoma/translocated liposarcoma (FUS/TLS or FUS) is concentrated within cytoplasmic stress granules under conditions of induced stress. Since only the mutants, but not endogenous wild-type FUS, are associated with most reported to date, relationship between FUS and represents a mutant-specific phenotype thus may be significance mutant-induced pathogenesis. While association mutant-FUS well established, effect mutant protein on has been...
DNA damage response (DDR) involves dramatic transcriptional alterations, the mechanisms of which remain ill defined. Here, we show that following genotoxic stress, RNA-binding motif protein 7 (RBM7) stimulates RNA polymerase II (Pol II) transcription and promotes cell viability by activating positive elongation factor b (P-TEFb) via its release from inhibitory 7SK small nuclear ribonucleoprotein (7SK snRNP). This is mediated activation p38MAPK, triggers enhanced binding RBM7 with core...
Plant pathogenic bacteria utilize an array of effector proteins to cause disease. Among them, transcriptional activator-like (TAL) effectors are unusual in the sense that they modulate transcription host. Although target genes and DNA specificity TAL have been elucidated, how control host is poorly understood. Previously, we showed Xanthomonas citri effectors, PthAs 2 3, preferentially targeted a citrus protein complex associated with repair. To extend our knowledge on mode action PthAs,...
The mRNAs that encode certain cytokines and proto-oncogenes frequently contain a typical AUrich motif is located in their 3'-untranslated region. protein AUF1 the first factor identified binds to AU rich regions mediates fast degradation of target mRNAs. exists as four different isoforms (p37, p40, p42 p45) are generated by alternative splicing. fact does not degrade mRNA itself had led suggestion other interacting proteins might be involved process selective degradation. Here we used yeast...
Abstract The fasciculation and elongation protein Zeta 1 (FEZ1) is the mammalian orthologue of Caenorhabditis elegans UNC‐76, which necessary for axon growth. Human FEZ1 interacts with Protein Kinase C (PKC) several regulatory proteins involved in functions ranging from microtubule associated transport to transcriptional regulation. Theoretical prediction, circular dichroism, fluorescence spectroscopy, limited proteolysis recombinant suggest that it contains disordered regions, especially...
NIP7 is one of the many trans-acting factors required for eukaryotic ribosome biogenesis, which interacts with nascent pre-ribosomal particles and dissociates as they complete maturation are exported to cytoplasm. By using conditional knockdown, we have shown previously that yeast Nip7p primarily 60S subunit synthesis while human involved in biogenesis 40S subunit. This raised possibility a different set proteins compared protein. two-hybrid system identified FTSJ3, putative ortholog Spb1p,...
Cancer cells exhibit modifications in nuclear architecture and transcriptional control. Tumor growth metastasis are supported by RUNX family scaffolding proteins, which mediate the assembly of nuclear-matrix-associated gene-regulatory hubs. We used proteomic analysis to identify RUNX2-dependent protein-protein interactions associated with matrix bone, breast prostate tumor cell types found that RUNX2 interacts three distinct proteins respond DNA damage - RUVBL2, INTS3 BAZ1B. Subnuclear foci...
Abstract The nuclear matrix bound transcription factor RUNX2 is a lineage‐specific developmental regulator that linked to cancer. We have previously shown controls of both RNA polymerase II genes and I‐dependent ribosomal genes. epigenetically retained through mitosis on classes target in condensed chromosomes. used fluorescence recovery after photobleaching measure the relative binding kinetics enhanced green fluorescent protein (EGFP)‐RUNX2 at sites nucleus nucleoli during interphase, as...
UAP56, ALY/REF, and NXF1 are mRNA export factors that sequentially bind at the 5' end of a nuclear but also reported to associate with exon junction complex (EJC). To screen for signal transduction pathways regulating assembly, we used fluorescence recovery after photobleaching measure binding EJC core proteins in complexes. The fraction tightly bound complexes was reduced by drug inhibition phosphatidylinositide 3-kinase (PI3 kinase)/AKT pathway, as eIF4A3, MAGOH, Y14. Inhibition mTOR...
The cytoplasmic and nuclear protein Ki-1/57 was first identified in malignant cells from Hodgkin's lymphoma. Despite studies showing its phosphorylation, arginine methylation, interaction with several regulatory proteins, the functional role of human remains to be determined. Here, we investigated relationship RNA functions. Through immunoprecipitation assays, verified association endogenous splicing proteins hnRNPQ SFRS9 HeLa cell extracts. We also found that recombinant able bind a poly-U...
mRNA export from the nucleus is critical for gene expression, but its regulation not well understood. We studied of by PI3Kinase/AKT pathway, a controller proliferation and survival often deregulated in cancer. UAP56, ALY/REF, NXF1 are factors that sequentially bind at TRanscription/EXport (TREX) complex 5′ end nuclear mRNA. subsequently binds pores mRNP export. used Fluorescence Recovery after Photobleaching (FRAP) to measure binding these complexes an integrated array actively transcribed...