A5003385846- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Herbal Medicine Research Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Banana Cultivation and Research
- Nuclear Structure and Function
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- thermodynamics and calorimetric analyses
- Legume Nitrogen Fixing Symbiosis
- Protist diversity and phylogeny
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Islanding Detection in Power Systems
- Cholinesterase and Neurodegenerative Diseases
Brazilian Center for Research in Energy and Materials
2011-2023
Brazilian Biosciences National Laboratory
2011-2023
Universidade Estadual Paulista (Unesp)
2017
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2011-2015
The nucleocapsid (N) protein of the SARS-CoV-2 virus, causal agent COVID-19, is a multifunction phosphoprotein that plays critical roles in virus life cycle, including transcription and packaging viral RNA. To play such diverse roles, N has two globular RNA-binding modules, N- (NTD) C-terminal (CTD) domains, which are connected by an intrinsically disordered region. Despite wealth structural data available for isolated NTD CTD, how these domains arranged full-length oligomerization...
Winged-helix transcriptional factors play important roles in the control of gene expression many organisms. In plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens, winged-helix protein BigR, a member ArsR/SmtB family metal sensors, regulates transcription bigR operon involved bacterial biofilm growth. Previous studies showed that BigR represses its own through occupation RNA polymerase-binding site; however, signals modulate activity biological function are still poorly...
Transcription activator-like (TAL) effectors from Xanthomonas species pathogens act as transcription factors in plant cells; however, how TAL activate host is unknown. We found previously that of the citrus canker pathogen citri, known PthAs, bind carboxyl-terminal domain sweet orange (Citrus sinensis) RNA polymerase II (Pol II) and inhibit activity CsCYP, a cyclophilin associated with Pol functions negative regulator cell growth. Here, we show PthA4 specifically interacted MAF1 (CsMAF1)...
Plant pathogenic bacteria utilize an array of effector proteins to cause disease. Among them, transcriptional activator-like (TAL) effectors are unusual in the sense that they modulate transcription host. Although target genes and DNA specificity TAL have been elucidated, how control host is poorly understood. Previously, we showed Xanthomonas citri effectors, PthAs 2 3, preferentially targeted a citrus protein complex associated with repair. To extend our knowledge on mode action PthAs,...
Abstract The nucleocapsid (N) protein plays critical roles in coronavirus genome transcription and packaging, representing a key target for the development of novel antivirals, which structural information on ligand binding is scarce. We used fluorescence polarization assay to identify small molecules that disrupt N RNA derived from SARS-CoV-2 packaging signal. Several phenolic compounds, including L-chicoric acid (CA), were identified as high-affinity N-protein ligands. CA was confirmed by...
Abstract Citrus cancer, caused by strains of Xanthomonas citri (Xc) and aurantifolii (Xa), is one the most economically important citrus diseases. Although our understanding molecular mechanisms underlying canker development has advanced remarkably in recent years, exactly how plants fight against these pathogens remains largely unclear. Using a Xa pathotype C strain that infects Mexican lime only sweet oranges as pathosystem to study immune response triggered this bacterium hosts, we herein...
Abstract The nucleocapsid (N) protein plays critical roles in coronavirus genome transcription and packaging, representing a key target for the development of novel antivirals, which structural information on ligand binding is scarce. We used fluorescence polarization assay to identify small molecules that disrupt N RNA derived from SARS-CoV-2 packaging signal. Several phenolic compounds, including L-chicoric acid (CA), were identified as high-affinity N-protein ligands. CA was confirmed by...
Abstract The nucleocapsid (N) protein of the SARS-CoV-2 virus, causal agent COVID-19, is a multifunction phosphoprotein that plays critical roles in virus life cycle, including transcription and packaging viral RNA. To play such diverse roles, N has two globular RNA-binding modules, N-(NTD) C-terminal (CTD) domains, which are connected by an intrinsically disordered region. Despite wealth structural data available for isolated NTD CTD, how these domains arranged full-length oligomerization...