A5026921228- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Phytoplasmas and Hemiptera pathogens
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Plant Stress Responses and Tolerance
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Signaling Pathways in Disease
- Sexual Differentiation and Disorders
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Sperm and Testicular Function
- Plant Virus Research Studies
- Toxin Mechanisms and Immunotoxins
- Banana Cultivation and Research
- Cancer Research and Treatments
- Forest Insect Ecology and Management
- SARS-CoV-2 and COVID-19 Research
- Herbal Medicine Research Studies
- Polysaccharides and Plant Cell Walls
Brazilian Biosciences National Laboratory
2014-2025
Brazilian Center for Research in Energy and Materials
2013-2023
Universidade Estadual de Campinas (UNICAMP)
1998-2018
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2010-2018
Piedmont International University
2013
Brazilian Synchrotron Light Laboratory
2002-2013
GTx (United States)
2013
Agruicultural Research Institute
2011
University of Parma
2008
General Department of Preventive Medicine
2000
The phytotoxin coronatine and the plant growth regulator methyl jasmonate (MeJA) caused similar growth-inhibitory effects on Arabidopsis seedlings. To test whether these two compounds have action, 14 independent coi1 (coronatine-insensitive) mutants of were selected. segregated as single recessive Mendelian markers, all alleles at locus. All also insensitive to MeJA male sterile. Both inhibited root growth, stimulated anthocyanin accumulation, increased level proteins ~31 ~29 kD detected in...
A cDNA clone (AtPUMP) encoding a plant uncoupling mitochondrial protein was isolated from Arabidopsis thaliana . The contains an open reading frame of 921 nucleotides 306 amino acids (predicted molecular weight 32 708). predicted polypeptide is 81% identical and 89% similar to the potato UCP‐like protein, includes energy transfer motif common transporters. AtPUMP gene exists as single copy in genome. corresponding transcript expressed all tissues strongly induced by cold treatment. We...
The phytotoxin coronatine and the plant growth regulator methyl jasmonate (MeJA) inhibit of Arabidopsis seedlings. Coronatine MeJA induced accumulation an approximately 29-kD protein in wild-type seedlings but not coi1 mutant, which is insensitive to both compounds. was recognized only by antibodies raised against partially purified polypeptide, also vegetative storage proteins (VSPs) from soybean (29 kD) poplar (32 kD). In absence added MeJA/coronatine, VSP-like highly expressed flowers...
Abstract Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that diverse economically relevant Xanthomonas bacteria endowed with xyloglucan depolymerization machinery is linked to pathogenesis. Using citrus canker pathogen model organism, show this system encompasses distinctive glycoside hydrolases, modular acetylesterase specific membrane transporters, demonstrating...
The nucleocapsid (N) protein of the SARS-CoV-2 virus, causal agent COVID-19, is a multifunction phosphoprotein that plays critical roles in virus life cycle, including transcription and packaging viral RNA. To play such diverse roles, N has two globular RNA-binding modules, N- (NTD) C-terminal (CTD) domains, which are connected by an intrinsically disordered region. Despite wealth structural data available for isolated NTD CTD, how these domains arranged full-length oligomerization...
Xanthomonas axonopodis pv. citri (Xac) and aurantifolii pathotype C (Xaa) are responsible for citrus canker disease; however, while Xac causes on all varieties, Xaa is restricted to Mexican lime, in sweet oranges it triggers a defence response. To gain insights into the differential pathogenicity exhibited by survey early molecular events leading development, detailed transcriptional analysis of orange plants infected with pathogens was performed. Using display, suppressed subtractive...
Winged-helix transcriptional factors play important roles in the control of gene expression many organisms. In plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens, winged-helix protein BigR, a member ArsR/SmtB family metal sensors, regulates transcription bigR operon involved bacterial biofilm growth. Previous studies showed that BigR represses its own through occupation RNA polymerase-binding site; however, signals modulate activity biological function are still poorly...
Transcriptional activator-like (TAL) effectors, formerly known as the AvrBs3/PthA protein family, are DNA-binding effectors broadly found in Xanthomonas spp. that transactivate host genes upon injection via bacterial type three-secretion system. Biologically relevant targets of TAL i.e. whose induction is vital to establish a compatible interaction, have been reported for xanthomonads colonize rice and pepper; however, citrus modulated by PthA"s" PthC"s" canker bacteria citri (Xc)...
The Citrus sinensis LATERAL ORGAN BOUNDERIES 1 (CsLOB1) gene, which is directly induced by the Xanthomonas citri subsp. effector PthA4, functions as a transcription factor and citrus canker susceptibility (S) gene. Genome editing of CsLOB1 has been shown to confer resistance disease. Previous studies revealed that CsLOB1-INDUCED EXPANSIN gene (CsLIEXP1) highly upregulated in citri-infected plants. Because expansins are associated with cell wall loosening, potentially facilitating bacterial...
Coronatine is a phytotoxin produced by some plant-pathogenic bacteria. It has been shown that coronatine mimics the action of methyl jasmonate (MeJA) in plants. MeJA plant-signaling molecule involved stress responses such as wounding and pathogen attack. In Arabidopsis thaliana, essential for pollen grain development. The coi1 (for coronatine-insensitive) mutant Arabidopsis, which insensitive to MeJA, produces sterile male flowers shows an altered response wounding. When differential display...
Abstract The Arabidopsis gene ATHCOR1, which encodes the CORI1 (coronatine-induced) protein, was expressed in bacterial cells. Soluble recombinant purified and shown to possess chlorophyllase (Chlase) activity vitro. To determine its vivo, wild-type coi1 mutant, lacksATHCOR1 transcripts, were transformed with sense antisense forms of gene. Wild-type plants overexpressing ATHCOR1 showed increased contents chlorophyllide (Chlide) without a substantial change total amount extractable...
Xanthomonas axonopodis pv. citri utilizes the type III effector protein PthA to modulate host transcription promote citrus canker. proteins belong AvrBs3/PthA family and carry a domain comprising tandem repeats of 34 amino acids that mediates protein-protein protein-DNA interactions. We show here variants PthAs from single bacterial strain localize nucleus plant cells form homo- heterodimers through association their repeat regions. hypothesize might also interact with distinct targets....
Transcription activator-like (TAL) effectors from Xanthomonas species pathogens act as transcription factors in plant cells; however, how TAL activate host is unknown. We found previously that of the citrus canker pathogen citri, known PthAs, bind carboxyl-terminal domain sweet orange (Citrus sinensis) RNA polymerase II (Pol II) and inhibit activity CsCYP, a cyclophilin associated with Pol functions negative regulator cell growth. Here, we show PthA4 specifically interacted MAF1 (CsMAF1)...
Many plant pathogenic bacteria rely on effector proteins to suppress defense and manipulate host cell mechanisms cause disease. The protein PthA modulates the transcriptome promote citrus canker. possesses unusual architecture with an internal region encompassing variable numbers of near-identical tandem repeats 34 amino acids termed repeat domain. This domain mediates protein-protein protein-DNA interactions, two polymorphic residues in each unit determine DNA specificity. To gain insights...
Plant pathogenic bacteria utilize an array of effector proteins to cause disease. Among them, transcriptional activator-like (TAL) effectors are unusual in the sense that they modulate transcription host. Although target genes and DNA specificity TAL have been elucidated, how control host is poorly understood. Previously, we showed Xanthomonas citri effectors, PthAs 2 3, preferentially targeted a citrus protein complex associated with repair. To extend our knowledge on mode action PthAs,...
Mitogen-activated protein kinases (MAPK) play crucial roles in plant immunity. We previously identified a citrus MAPK (CsMAPK1) as differentially expressed response to infection by Xanthomonas aurantifolii, bacterium that causes canker Mexican lime but hypersensitive reaction sweet oranges. Here, we confirm that, orange, CsMAPK1 is rapidly and preferentially induced X. aurantifolii relative citri. To investigate the role of resistance, plants under control PR5 gene promoter, which wounding....
Citrus canker, caused by Xanthomonas citri, affects most commercial citrus varieties. All X. citri strains possess at least one transcription activator-like effector of the PthA family that activates host disease susceptibility (S) genes. The strain 306 encodes four effectors; nevertheless, only PthA4 is known to elicit cankers on citrus. As none PthAs act as avirulence factors citrus, we hypothesized 1-3 might also contribute pathogenicity certain hosts. Here, show that, although...
To cope with toxic levels of H
Salicylic acid (SA) and its methyl ester, salicylate (MeSA) are well known inducers of local systemic plant defense responses, respectively. MeSA is a major mediator acquired resistance (SAR) conversion back into SA thought to be required for SAR. In many species, mediated by esterases the SABP2 family. Here we show that Citrus sinensis homologue protein CsMES1 catalyzes hydrolysis SA. Molecular modeling studies suggest shares same structure SA-binding mode with tobacco SABP2. However, an...