A5024294809- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- ATP Synthase and ATPases Research
- Corneal Surgery and Treatments
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Genetics, Bioinformatics, and Biomedical Research
- bioluminescence and chemiluminescence research
- Biochemical and Molecular Research
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- Shoulder Injury and Treatment
- RNA Research and Splicing
- IL-33, ST2, and ILC Pathways
- Developmental Biology and Gene Regulation
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
Universidade de São Paulo
2015-2025
Broad Institute
2022-2025
Institute of Biomedical Science
2016-2021
Hospitais da Universidade de Coimbra
2018
Universidade Federal de São Paulo
2008-2017
University of Liège
2013
// Thaiomara A. Silva 1, * , Basílio Smuczek Iuri C. Valadão Luciana M. Dzik 1 Rebeca P. Iglesia Mário Cruz 2 André Zelanis 3, 4 Adriane S. de Siqueira Solange M.T. Serrano Gary Goldberg 5 Ruy G. Jaeger Vanessa Freitas Department of Cell and Developmental Biology, Institute Biomedical Sciences (ICB), University Sao Paulo, Brazil Center Facilities Support Research, 3 Science Technology, Federal Paulo (ICT-UNIFESP), Jose dos Campos, Special Laboratory Applied...
Mesenchymal stromal cells (MSCs) are multipotent with abilities to exert immunosuppressive response promoting tissue repair. Studies have shown that MSCs can secrete extracellular vesicles (MVs-MSCs) similar regulatory functions the parental cells. Furthermore, strong evidence suggesting MVs-MSCs modulate several immune (i.e., Th1, Th17, and Foxp3+ T cells). However, their precise effect on macrophages (Mϕs) remains unexplored. We investigated immunoregulatory of activated M1-Mϕs in vitro...
Abstract Image‐based profiling quantitatively assesses the effects of perturbations on cells by capturing a breadth changes via microscopy. Here, we provide two complementary protocols to help explore and interpret data from image‐based experiments. In first protocol, examine similarity among perturbed cell samples using compounds that cluster their mechanisms action. The protocol includes steps feature‐driving differences between visualize correlations features treatments create...
Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence codes a 21kDa protein selected. After cloning this sequence, the results it ubiquitous by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells dose-dependent manner. Pretreatment host with P21-His(6) inhibited cell invasion amastigotes from G CL strains. On other...
Background P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim this study was to determine the effect recombinant based on (P21-His6) inflammatory macrophages during phagocytosis. Findings Our results showed that P21-His6 acts as phagocytosis inducer by binding CXCR4 chemokine receptor and activating actin polymerization way dependent onthe PI3-kinase signaling pathway. Conclusions Thus, our shed light notion native component related T. cruzi evasion...
Mesenchymal stromal cells (MSCs) can generate immunological tolerance due to their regulatory activity in many immune cells. Extracellular vesicles (EVs) release is a pivotal mechanism by which MSCs exert actions. In this study, we evaluate whether mesenchymal cell extracellular (MSC-EVs) modulate T response. were expanded and EVs obtained differential ultracentrifugation of the supernatant. The incorporation MSC-EVs was detected confocal microscopy. Expression surface markers flow cytometry...
Trypanosoma cruzi is a protozoan parasite that comprises different phylogenetic groups and the causative agent of Chagas' disease. Different T. strains present differences in infectivity vitro vivo experimental models, which are likely related to expression virulence factors. Amastin surface glycoprotein abundantly expressed on intracellular mammalian amastigote form parasite. In this study, we showed highly infective strain (G strain) extracellular (EA) invasive forms reduced RNA levels...
Abstract The role of autophagy in cell death is still controversial and a lot debate has concerned the transition from its pro-survival to pro-death roles. similar structure triterpenoids Betulinic (BA) Oleanolic (OA) acids allowed us prove that this involves parallel damage mitochondria lysosome. After treating immortalized human skin keratinocytes (HaCaT) with either BA or OA, we evaluated viability, proliferation mechanism death, function morphology lysosomes status flux. We also...
Abstract Mevalonate kinase (MVK) is an essential enzyme acting in early steps of sterol isoprenoids biosynthesis, such as cholesterol humans or ergosterol trypanosomatids. MVK conserved from bacteria to mammals and localizes glycosomes During the course T. cruzi characterization, we found that, addition glycosomes, this may be secreted modulate cell invasion. To evaluate role TcMVK parasite-host interactions, recombinant protein was produced anti-TcMVK antibodies were raised mice. detected...
Abstract Uric acid (UA), a product of purine nucleotide degradation able to initiate an immune response, represents breakpoint in the evolutionary history humans, when uricase, enzyme required for UA cleavage, was lost. Despite being inert human cells, its soluble form (sUA) can increase level interleukin-1β (IL-1β) murine macrophages. We, therefore, hypothesized that recognition sUA is achieved by Naip1-Nlrp3 inflammasome platform. Through structural modelling predictions and transcriptome...
Laminin peptides influence tumor behavior. In this study, we addressed whether laminin peptide C16 (KAFDITYVRLKF, γ1 chain) would increase invadopodia activity of cells from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). We found that stimulates over time in both lines. Rhodamine-conjugated decorates the edge cells, suggesting a possible binding to membrane receptors. Flow cytometry showed increases activated β1 integrin, integrin miRNA-mediated depletion diminishes C16-induced...
Until 2015, loss-of-function studies to elucidate protein function in Leishmania relied on gene disruption through homologous recombination. Then, the CRISPR/Cas9 revolution reached these protozoan parasites allowing efficient genome editing with one round of transfection. In addition, development LeishGEdit, a PCR-based toolkit for generating knockouts and tagged lines using CRISPR/Cas9, allowed more straightforward effective editing. this system, plasmid pTB007 is delivered episomal...
Cisplatin is an antineoplastic agent used to treat various tumors. In mammals, it can cause nephrotoxicity, tissue damage, and inflammation. The release of inflammatory mediators leads the recruitment infiltration immune cells, particularly neutrophils, at site often as inducer acute kidney injury (AKI) in experimental models, including zebrafish (Danio rerio), due its accumulation cells. Current protocols larval focus on studying effect AKI but ignore other systematic outcomes. this study,...
ASCL1 is a transcription factor that directs neural progenitors towards lineage differentiation. Although many of the molecular mechanisms underlying its action have been described, several targets remain unidentified. We identified in chick genome putative enhancer (cE1) upstream Scratch2 ( Scrt2 ) locus with predicted heterodimerization motif for and POU3F2. In this study, we investigated role regulating expression embryonic spinal cord. confirmed cE1 region interacted promoter. was...
Abstract We herein describe a postdoctoral training program designed to train biologists with microscopy experience in bioimage analysis. detail the rationale behind program, various components of and outcomes terms works produced career effects on past participants. analyze results an anonymous survey distributed present participants, indicating overall high value all 12 rated aspects but significant heterogeneity which were most important each participant. Finally, we propose this model as...
Abstract Autofluorescence microscopy uses intrinsic sources of molecular contrast to provide cellular-level information without extrinsic labels. However, traditional cell segmentation tools are often optimized for high signal-to-noise ratio (SNR) images, such as fluorescently labeled cells, and unsurprisingly perform poorly on low SNR autofluorescence images. Therefore, new needed microscopy. Cellpose is a deep learning network that generalizable across diverse images automatically segments...
In Brazil, cutaneous leishmaniasis is caused predominantly by L. (V.) braziliensis. The few therapeutic drugs available exhibit several limitations, mainly related to drug toxicity and reduced efficacy in some regions. Miltefosine (MF), the only oral for treatment, not widely has yet been approved human use Brazil. Our group previously reported existence of differential susceptibility among braziliensis clinical isolates. this work, we further characterized three these isolates chosen...