A5010474113- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- Renal and Vascular Pathologies
- Neurological Complications and Syndromes
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- Heme Oxygenase-1 and Carbon Monoxide
- Acute Kidney Injury Research
- Transplantation: Methods and Outcomes
- Renal Diseases and Glomerulopathies
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Eicosanoids and Hypertension Pharmacology
- Dialysis and Renal Disease Management
- Tissue Engineering and Regenerative Medicine
- Adenosine and Purinergic Signaling
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Inflammatory mediators and NSAID effects
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- MicroRNA in disease regulation
Hospital Israelita Albert Einstein
2015-2024
Universidade Federal de São Paulo
2015-2024
Sociedade de Nefrologia do Estado de São Paulo
2023
Hospital do Rim e Hipertensão
2002-2020
Universidade de São Paulo
2006-2018
Hospital São Paulo
2001-2017
Brigham and Women's Hospital
1992-2011
Jean Mayer Human Nutrition Research Center on Aging
2011
National Institute of Diabetes and Digestive and Kidney Diseases
2011
Rhode Island Hospital
2011
Abstract Mesenchymal stem cells (MSCs) have regenerative properties in acute kidney injury, but their role chronic diseases is still unknown. More specifically, it not known whether MSCs halt fibrosis. The purpose of this work was to investigate the fibrogenesis using a model renal failure. were obtained from tibias and femurs male Wistar-EPM rats. Female Wistar rats subjected remnant model, 2|×|105 intravenously administrated each rat every other week for 8 weeks or only once followed 12...
Background— Kidney transplant recipients, like other patients with chronic kidney disease, experience excess risk of cardiovascular disease and elevated total homocysteine concentrations. Observational studies suggest increased is a factor for disease. The impact lowering levels in recipients unknown. Methods Results— In double-blind controlled trial, we randomized 4110 stable to multivitamin that included either high dose (n=2056) or low (n=2054) folic acid, vitamin B6, B12 determine...
Adipose-derived mesenchymal stem cells (ADMSCs) display immunosuppressive properties, suggesting a promising therapeutic application in several autoimmune diseases, but their role type 1 diabetes (T1D) remains largely unexplored. The aim of this study was to investigate the immune regulatory properties allogeneic ADMSC therapy T cell–mediated NOD mice. treatment reversed hyperglycemia early-onset 78% diabetic mice, and effect associated with higher serum insulin, amylin, glucagon-like...
The aim of this study was to investigate the role TLR2, TLR4 and MyD88 in sepsis-induced AKI. C57BL/6 TLR2−/−, TLR4−/− MyD88−/− male mice were subjected sepsis by cecal ligation puncture (CLP). Twenty four hours later, kidney tissue blood samples collected for analysis. that CLP had preserved renal morphology, fewer areas hypoxia apoptosis compared with wild-type (WT). completely protected WT mice. We also observed reduced expression proinflammatory cytokines kidneys knockout those...
Inflammation contributes to the pathogenesis of chronic kidney disease (CKD). Molecules released by inflamed injured tissue can activate toll-like receptors (TLRs), thereby modulating macrophage and CD4(+) T-cell activity. We propose that in renal fibrogenesis, M2 macrophages are recruited activated a T helper subset 2 cell (T(H)2)-prone inflammatory milieu MyD88-dependent manner. Mice submitted unilateral ureteral ligation (UUO) demonstrated an increase infiltration with collagen deposition...
Background Kidney transplant (KT) recipients are considered a high-risk group for unfavorable outcomes in the course of coronavirus disease 2019 (COVID-19). Aim To describe clinical aspects and COVID-19 among KT recipients. Methods This multicenter cohort study enrolled 1,680 diagnosed with between March November 2020, from 35 Brazilian centers. The main outcome was 90-day cumulative incidence death, entire according to acute kidney injury (AKI) renal replacement therapy (RRT) requirement....
Background. Cardiovascular disease is the leading cause of death in renal transplant recipients. The purpose this study was to determine effects a successful transplantation on left ventricular morphology and function 24-hr blood pressure profile. Methods. Twenty-four patients with end-stage were prospectively studied by ambulatory monitoring echocardiography before at 3, 6, 12 months after transplantation. Patients also analyzed according their Results. We observed significant drop mean...
Polymerase chain reaction-assisted reverse transcription was used to study the temporal course of rejection in unmodified recipients murine pancreatic islet cell allografts (DBA/2-->B6AF1) by using syngeneic tissues as controls. The histologic appearance grafts analyzed parallel. Preproinsulin and constant region TCR-beta transcripts were studied markers graft integrity infiltrating T mass, respectively. participation certain cytokines CTL detection IL-2, IFN-gamma, IL-4, CTL-specific serine...
Tubule-interstitial nephritis (TIN) results in decreased renal function and interstitial inflammation, which ultimately leads to fibrosis. Excessive adenine intake can cause TIN because xanthine dehydrogenase (XDH) convert this purine into an insoluble compound, precipitates the tubuli. Innate immune sensors, such as Toll-like receptors (TLR) inflammasome complex, play a crucial role initiation of inflammation. The aim study was evaluate roles TLR-2 -4, Myd88 complex experimental model TIN....
Adipose tissue-derived stem cells (ASCs) are an attractive source of with regenerative properties that similar to those bone marrow cells. Here, we analyze the role ASCs in reducing progression kidney fibrosis. Progressive renal fibrosis was achieved by unilateral clamping pedicle mice for 1 h; after that, reperfused immediately. Four hours surgery, 2 × 10(5) were intraperitoneally administered, and followed 24 h posttreatment then at some other time interval next 6 weeks. Also, animals...
Background The tubule-interstitial fibrosis is the hallmark of progressive renal disease and strongly associated with inflammation this compartment. Heme-oxygenase-1 (HO-1) a cytoprotective molecule that has been shown to be beneficial in various models injury. However, role HO-1 reversing an established scar not yet addressed. Aim We explored ability halt reverse establishment experimental model chronic disease. Methods Sprague-Dawley male rats were subjected unilateral ureteral obstruction...
Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and vivo. Their use for therapy the transplant field is attractive as they could render of drugs unnecessary. The aim this study was to investigate effect ADSC on prolonging skin allograft survival. Animals that were treated with a single injection donor allogeneic one day after transplantation showed an increase graft survival by approximately week. This improvement associated preserved...
Acute kidney injury (AKI) is considered an inflammatory disease in which toll-like receptors (TLRs) signaling pathways play important role. The activation of TLRs results production several cytokines leading to further renal damage. In contrast, are key players on autophagy induction, associated with a protective function cisplatin-induced AKI. Hence, the present study aimed evaluate specific participation TLR2 and TLR4 molecules development Complementarily, we also investigated link between...
Mesenchymal stromal cells (MSCs) are multipotent with abilities to exert immunosuppressive response promoting tissue repair. Studies have shown that MSCs can secrete extracellular vesicles (MVs-MSCs) similar regulatory functions the parental cells. Furthermore, strong evidence suggesting MVs-MSCs modulate several immune (i.e., Th1, Th17, and Foxp3+ T cells). However, their precise effect on macrophages (Mϕs) remains unexplored. We investigated immunoregulatory of activated M1-Mϕs in vitro...
Acute and chronic kidney injuries (AKI CKI) constitute syndromes responsible for a large part of renal failures, are today still associated with high mortality rates. Given the lack more effective therapies, there has been intense focus on use stem cells organ protective regenerative effects. Mesenchymal (MSCs) have shown great potential in treatment various diseases immune character, although is debate its mechanism action. Thus, greater understanding role MSCs, we evaluated effect adipose...
Abstract: Background: The widespread use of prophylactic ganciclovir and anti‐lymphocyte/thymocyte therapies are associated with increased induction ganciclovir‐resistant cytomegalovirus (CMV) strains. sirolimus has been a lower incidence CMV infection in transplant recipients. We questioned whether it could also be effective as therapeutic treatment resistant infection. Methods: Patients infections determined clinically by DNA sequencing analysis were enrolled. Antigenaemia used to...
Ischemia and reperfusion injury (IRI) contributes to the development of chronic interstitial fibrosis/tubular atrophy in renal allograft patients. Cyclooxygenase (COX) 1 2 actively participate acute ischemic by activating endothelial cells inducing oxidative stress. Furthermore, blockade COX has been associated with organ improvement after damage. The aim this study was evaluate role fibrosis performing a immediately before IRI. We subjected C57Bl/6 male mice 60 min unilateral pedicle...
Ischemia/reperfusion injury (IRI) is a leading cause of acute renal failure. The definition the molecular mechanisms involved in IRI and counter protection promoted by ischemic pre-conditioning (IPC) or Hemin treatment an important milestone that needs to be accomplished this research area. We examined, through oligonucleotide microarray protocol, differential transcriptome profiles mice submitted IRI, IPC treatment. After identifying differentially expressed genes observed for each...
Mesenchymal stromal cells (MSCs) can generate immunological tolerance due to their regulatory activity in many immune cells. Extracellular vesicles (EVs) release is a pivotal mechanism by which MSCs exert actions. In this study, we evaluate whether mesenchymal cell extracellular (MSC-EVs) modulate T response. were expanded and EVs obtained differential ultracentrifugation of the supernatant. The incorporation MSC-EVs was detected confocal microscopy. Expression surface markers flow cytometry...