A5033847158- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Animal Genetics and Reproduction
- Escherichia coli research studies
- Sperm and Testicular Function
- Bioinformatics and Genomic Networks
- Reproductive Biology and Fertility
- BRCA gene mutations in cancer
- Genetic and phenotypic traits in livestock
- Chromosomal and Genetic Variations
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Genetics and Neurodevelopmental Disorders
- Diabetes and associated disorders
- Enterobacteriaceae and Cronobacter Research
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Gene Regulatory Network Analysis
- Immune Cell Function and Interaction
- Hormonal and reproductive studies
- Rural Development and Agriculture
- Cystic Fibrosis Research Advances
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2014-2023
Universidade de São Paulo
2011-2022
Hospital Universitário da Universidade de São Paulo
2008-2022
Baylor College of Medicine
2020
National Institutes of Health
2020
University of California, San Diego
2020
UPMC Health System
2020
Penn Presbyterian Medical Center
2020
Genomic (Brazil)
2016
Hospital Israelita Albert Einstein
2004-2014
Abstract We have performed cDNA microarray analyses to identify gene expression differences between highly invasive glioblastoma multiforme (GBM) and typically benign pilocytic astrocytomas (PA). Despite the significant clinical pathological 2 tumor types, only 63 genes were found exhibit 2‐fold or greater overexpression in GBM as compared PA. Forty percent of these are related regulation cell cycle mitosis. QT‐PCR validation 6 overexpressed genes: MELK , AUKB ASPM PRC1 IL13RA2 KIAA0101...
Aims: Cyanobacteria-deprived lichens of the species Canoparmelia caroliniana, crozalsiana, texana, Parmotrema sancti-angeli and tinctorum were screened for presence chemo-organotrophic nitrogen-fixing bacteria. Methods Results: Fifty-three lichen samples subjected to enrichment selection using a nitrogen-free minimal medium positive acetylene reduction. Seventeen isolates, able fix nitrogen, belonged Gamma-proteobacteria group identified as: Acinetobacter sp., Pantoea Pseudomonas stutzeri,...
A Gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium, designated ICB 89(T), was isolated from stems of a Brazilian sugar cane variety widely used in organic farming. 16S rRNA gene sequence analysis revealed that strain 89(T) belonged to the genus Stenotrophomonas most closely related maltophilia LMG 958(T), rhizophila 22075(T), nitritireducens L2(T), [Pseudomonas] geniculata ATCC 19374(T), hibiscicola 19867(T) beteli 19861(T). DNA-DNA hybridization together with...
Germline mutations in BRCA1, BRCA2 and TP53 genes have been identified as one of the most important disease-causing issues young breast cancer patients worldwide. The specific defective biological processes that trigger germline mutation-associated -negative tumors remain unclear. To delineate an initial portrait Brazilian early-onset cancer, we performed investigation combining both tumor analysis. screening BRCA2, CHEK2 (c.1100delC) was 54 unrelated <35 y; their were investigated with...
Abstract Sex differences in the prevalence of psychiatric disorders are well documented, with exposure to stress during gestation differentially impacting females and males. We explored sex-specific DNA methylation cord blood 39 32 males born at term appropriate weight birth regarding their potential connection outcomes. Mothers were interviewed gather information about environmental factors (gestational exposure) that could interfere profiles newborns. Bisulphite converted was hybridized...
The virulence profiles of most atypical enteropathogenic Escherichia coli (EPEC) strains are unknown. A total 118 typical and EPEC serotypes non-EPEC serogroups isolated from children with or without acute diarrhea who were different cities in Brazil examined for virulence-associated markers adherence to HEp-2 cells, also had random amplified polymorphic DNA (RAPD) analysis performed. Atypical identical regard the factors encoded on locus enterocyte effacement (LEE). In contrast strains,...
The Down syndrome (DS) immune phenotype is characterized by thymus hypotrophy, higher propensity to organ-specific autoimmune disorders, and susceptibility infections, among other features. Considering that AIRE (autoimmune regulator) located on 21q22.3, we analyzed protein gene expression in surgically removed thymuses from 14 DS patients with congenital heart defects, who were compared 42 age-matched controls anomaly as an isolated malformation. Immunohistochemistry revealed 70.48 ± 49.59...
Denys-Drash and Frasier syndromes are rare human disorders that associate nephropathy with gonadal genital abnormalities. In DDS there is a predisposition to Wilms' tumor. Heterozygous point mutations in the tumor, type1 gene (WT1), particularly those altering zinc finger (ZF) encoding exons, have been reported most patients, while intron 9 of same cause FS. This paper describes two cases DDS, one FS patient Wilm's tumor intersex genitalia, which were searched by sequencing exons 8 WT1 gene....
Ischemia/reperfusion injury (IRI) is a leading cause of acute renal failure. The definition the molecular mechanisms involved in IRI and counter protection promoted by ischemic pre-conditioning (IPC) or Hemin treatment an important milestone that needs to be accomplished this research area. We examined, through oligonucleotide microarray protocol, differential transcriptome profiles mice submitted IRI, IPC treatment. After identifying differentially expressed genes observed for each...
Abstract Biological networks display high robustness against random failures but are vulnerable to targeted attacks on central nodes. Thus, network topology analysis represents a powerful tool for investigating susceptibility node removal. Here, we built protein interaction associated with chemoresistance temozolomide, an alkylating agent used in glioma therapy and analyzed their modular structure intentional attack. These showed functional modules related DNA repair, immunity, apoptosis,...
Host population size, density, immune status, age structure, and contact rates are critical elements of virus epidemiology. Slum populations stand out from other settings may present differences in the epidemiology acute viral infections. We collected nasopharyngeal specimens 282 children aged ≤5 years with respiratory tract infection (ARI) during 2005 to 2006 one largest Brazilian slums. conducted real-time reverse transcription-polymerase chain reaction (RT-PCR) for 16 viruses, nested...
Fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. Ability to fix nitrogen was verified by the acetylene reduction assay. All potentially tested showed positive hybridization signals with a nifH probe derived Azospirillum brasilense. The characterized RAPD, ARDRA 16S rDNA sequence analysis. RAPD analyses revealed 8 unique genotypes, remaining 6...
Oral squamous cell carcinoma (OSCC) is a frequent neoplasm, which usually aggressive and has unpredictable biological behavior unfavorable prognosis. The comprehension of the molecular basis this variability should lead to development targeted therapies as well improvements in specificity sensitivity diagnosis. Samples primary OSCCs their corresponding surgical margins were obtained from male patients during surgery gene expression profiles screened using whole-genome microarray technology....
About 5-10% of breast and ovarian carcinomas are hereditary most these result from germline mutations in the BRCA1 BRCA2 genes. In women Ashkenazi Jewish ascendance, up to 30% may be attributable genes, where 3 founder mutations, c.68_69del (185delAG) c.5266dup (5382insC) c.5946del (6174delT) BRCA2, commonly encountered. It has been suggested by some authors that screening for should undertaken all Brazilian with cancer. Thus, goal this study was determine prevalence three identified...
Mesenchymal stromal cells (MSCs) orchestrate tissue repair by releasing cell-derived microvesicles (MVs), that presumably small RNA species, modulate global gene expression. The knowledge of miRNA/mRNA signatures linked to a reparative status may elucidate some the molecular events associated with MSC protection. Here, we used model cisplatin-induced kidney injury (AKI) assess how MSCs or MVs could restore function. and presented similar protective effects, which were evidenced in vivo vitro...
Parkinson’s disease (PD)—classically characterized by severe loss of dopaminergic neurons in the substantia nigra pars compacta—has a caudal-rostral progression, beginning dorsal motor vagal nucleus and, less extent, olfactory system, progressing to midbrain and eventually basal forebrain neocortex. About 90% cases are idiopathic. To study molecular mechanisms involved idiopathic PD we conducted comparative transcriptional interaction networks (VA), locus coeruleus (LC), (SN) Braak stages...
Background Changes in innate and adaptive immunity occurring in/around pancreatic islets had been observed peripheral blood mononuclear cells (PBMC) of Caucasian T1D patients by some, but not all researchers. The aim our study was to investigate whether gene expression patterns PBMC the highly admixed Brazilian population could add knowledge about pathogenic mechanisms. Methods We assessed global from two groups matched for age, sex BMI: 20 with recent-onset (≤ 6 months diagnosis, a time...
Abstract Two agonadic sisters, one with a 46,XY and the other 46,XX karyotype, both normal female external genitalia hypoplastic Müllerian derivatives, born to consanguineous marriage, were studied from clinical, endocrinological, histological, genetic perspective. Using PCR amplification, Southern hybridization, DGGE analysis, it was found that XY patient had no mutations in conserved sequence of SRY gene, putative testis‐determining gene mammals, whereas her XX affected sister is...