A5069284557- Research on Leishmaniasis Studies
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Trypanosoma species research and implications
- Electrochemical Analysis and Applications
- Biosensors and Analytical Detection
- Dendrimers and Hyperbranched Polymers
- Monoclonal and Polyclonal Antibodies Research
- Heat shock proteins research
- Toxin Mechanisms and Immunotoxins
- Analytical Chemistry and Sensors
- Synthesis and Biological Evaluation
- Insect and Pesticide Research
- Conducting polymers and applications
- Cannabis and Cannabinoid Research
- Molecular Junctions and Nanostructures
- Advanced Biosensing Techniques and Applications
- Protein Structure and Dynamics
- SARS-CoV-2 detection and testing
- Neurotransmitter Receptor Influence on Behavior
- Pharmacology and Obesity Treatment
- 3D Printing in Biomedical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Veterinary medicine and infectious diseases
- Parasitic Diseases Research and Treatment
Universidade Federal do Paraná
2019-2024
Universidade Estadual de Campinas (UNICAMP)
2015-2022
Delft University of Technology
2017
Brazilian Biosciences National Laboratory
2017
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2013-2015
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2012-2013
Abstract The use of biosensors in point‐of‐care (POC) testing devices has attracted considerable attention the past few years, mainly because their high specificity, portability, and relatively low cost. Coupling these with miniaturized electrochemical transducers shown great potential toward simple, rapid, cost‐effective analysis that can be performed field, especially for healthcare, environmental monitoring, food quality control. For this reason, number publications field grown...
Electrically active field-effect transistors (FET) based biosensors are of paramount importance in life science applications, as they offer direct, fast, and highly sensitive label-free detection capabilities several biomolecules specific interest. In this work, we report a detailed investigation on surface functionalization covalent immobilization biomarkers using biocompatible ethanolamine poly(ethylene glycol) derivate coatings, compared to the conventional approaches silica monoliths,...
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important track COVID-19 cases and the humoral response infections immunization future vaccines. The aim of this work was develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, quantitative detection human antibodies against serum, plasma, or blood. Recombinant 6xHis-tagged Nucleocapsid protein mobilized on surface Ni2+ beads challenged with serum blood samples obtained from...
Abstract This work presents a simple, fast and low‐cost method for simultaneous determination of acetylsalicylic acid (ASA), without alkaline hydrolysis caffeine (CF) in pharmaceutical formulations using boron‐doped diamond as the working electrode through differential pulse voltammetry. A good repeatability was reached 20 measurements, with low relative standard deviation less than 1.0 %. The calibration curves presented great linear correlation coefficient both drugs ( R =0.999) limit...
This work describes the synthesis of 1,2,3-triazole amino acid-derived-3-O-galactosides 1-6 and di-lactose-derived glycoconjugate 7 as potential galectin-3 inhibitors. The target compounds were synthesized by Cu(I)-catalyzed azide-alkyne cycloaddition reaction ('click chemistry') between azido-derived acids N3-ThrOBn, N3-PheOBn, N3-N-Boc-TrpOBn, N3-N-Boc-LysOBn, N3-O-tBu-AspOBn N3-l-TyrOH, corresponding alkyne-based sugar 3-O-propynyl-GalOMe, well click chemistry azido-lactose 2-propynyl...
Abstract Uric acid (UA), a product of purine nucleotide degradation able to initiate an immune response, represents breakpoint in the evolutionary history humans, when uricase, enzyme required for UA cleavage, was lost. Despite being inert human cells, its soluble form (sUA) can increase level interleukin-1β (IL-1β) murine macrophages. We, therefore, hypothesized that recognition sUA is achieved by Naip1-Nlrp3 inflammasome platform. Through structural modelling predictions and transcriptome...
Poly(3,4-ethylenedioxythiophene) nanotubes (PEDOT-NTs) were electrochemically synthesized onto a stainless steel mesh electrode followed by one-pot electrodeposition of gold nanoparticles (AuNPs) at the nanotubular surface. The hybrid nanomaterial has shown remarkable electrochemical properties with diminishment charge transfer resistance besides possibility further modification thiol bonding AuNPs. modified electrodes deeply characterized techniques, in special impedance spectroscopy, which...
This work describes the highly sensitive detection of organophosphorus pesticides employing cobalt(II) 4,4,4,4-tetrasulfo-phthalocyanine (CoTSPc) macrocycle complex, carbon nanotubes (CNT), and 1-methyl-3-octylimidazolium tetrafluoroborate (OMIM[BF4]). The technique is based on enzyme acetylcholinesterase (AChE) inhibition. composite was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, amperometry. AChE immobilized electrode surface...
An immunosensor was developed using a SAM of an alkanethiol associated with PAMAM(G4) dendrimers based on surface plasmon resonance (SPR) to enhance the sensitivity for troponin T detection in blood samples. The feasibility three-dimensional platforms development immunosensors demonstrated by evaluating three different generations these (G3, G4, and G5) detect T. results showed efficiency 3D anchoring biomolecules, amplifying sandwich assay good performance detection, secondary monoclonal...
In the present study, surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies for immunodiagnostics visceral leishmaniasis (VL). This was formed by union in same artificial coding DNA ten different peptides, which showed themselves reactive positive canine serum VL. Using CP10 enzyme-linked immunosorbent assays (ELISA), it...
In this study, polypyrrole nanotubes (PPy-NT) and gold nanoparticles (AuNPs) were electrochemically synthesized to form a hybrid material used as an electroactive layer for the attachment of proteins construction high-performance biosensor. Besides enhancement intrinsic conductivity PPy-NT, AuNPs act anchor group formation self-assembly monolayers (SAMs) from gold-sulfur covalent interaction between Mercaptopropionic acid (MPA). This was evaluate viability performance platform developed...
Abstract Opioid use disorder (OUD) is a public health problem that includes symptoms such as withdrawal syndrome and opioid-induced hyperalgesia (OIH). Currently, drugs to treat side effects of opioids also have undesirable effects, which lead limitations. This study investigated the effect treatment with cannabidiol (CBD) in morphine-induced signs morphine-dependent rats. Male female rats were submitted physical dependence protocol consisting twice daily morphine (7.89 mg/kg, 1ml/kg, s.c.)...
In this work we demonstrate the use of a dielectric barrier discharge plasma for treatment SU-8. The resulting hydrophilic surface displays 5° contact angle and (0.40 ± 0.012) nm roughness. Using technique also present proof concept IgG prostate specific antigen biodetection on thin layer SU-8 over gold via plasmon resonance detection.