A5043090360- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Amino Acid Enzymes and Metabolism
- Microbial Community Ecology and Physiology
- biodegradable polymer synthesis and properties
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
University of Tübingen
2020-2025
Czech Academy of Sciences, Institute of Microbiology
2025
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important track COVID-19 cases and the humoral response infections immunization future vaccines. The aim of this work was develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, quantitative detection human antibodies against serum, plasma, or blood. Recombinant 6xHis-tagged Nucleocapsid protein mobilized on surface Ni2+ beads challenged with serum blood samples obtained from...
The 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (iPGAM) has been identified as a regulating key point in the carbon storage metabolism of cyanobacteria. Upon nitrogen starvation, iPGAM is inhibited by PII-interacting regulator PirC, which released from its interaction partner PII due to elevated 2-oxoglutarate levels. In silico analysis 338 different iPGAMs revealed deep-rooted distinctive evolution Remarkably, cyanobacterial possess unique loop structure and an extended...
During phases of nitrogen starvation, the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 produces polyhydroxybutyrate (PHB). This polymer is high biotechnological relevance because its potential as biodegradable plastic. Analysis genome revealed an operon (slr0058-slr0061) containing several genes, which are putatively related to PHB metabolism. While Slr0058 show similarities with regulatory phasin PhaF, protein Slr0060 could serve intracellular depolymerase. Investigation...
The 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (iP-GAM) has been identified as a crucial regulating key point in the carbon storage metabolism of cyanobacteria. Upon nitrogen starvation, iPGAM is inhibited by PII-interacting regulator PirC, released from its interaction partner PII due to elevated 2-oxoglutarate levels. In-silico analysis 338 different iPGAMs revealed deep-rooted distinctive evolution Additionally, cyanobacterial possess unique loop structure and an extended...
ABSTRACT A technique allowing high throughput, fast and low-cost quantitative analysis of human IgG antibodies reacting to SARS-CoV-2 antigens will be required understand the levels protecting in population raised response infections and/or immunization. We described previously a fast, simple, inexpensive Ni 2+ magnetic bead immunoassay which allowed detection against nucleocapsid protein using minimal amount serum or blood. major drawback system was that it only processed 12 samples...