A5025026489- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Parkinson's Disease Mechanisms and Treatments
- COVID-19 epidemiological studies
- Hepatitis B Virus Studies
- Long-Term Effects of COVID-19
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- CAR-T cell therapy research
- Immune Response and Inflammation
- Vaccine Coverage and Hesitancy
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Animal Virus Infections Studies
- Protein Kinase Regulation and GTPase Signaling
- Tuberculosis Research and Epidemiology
- NF-κB Signaling Pathways
Natural and Medical Sciences Institute
2016-2025
University of Tübingen
2016-2025
Medizinische Hochschule Hannover
2022
Helmholtz Centre for Infection Research
2022
Center for Experimental and Clinical Infection Research
2022
Deutsche Post (Germany)
2022
German Center for Infection Research
2022
Johns Hopkins University
2017
Johns Hopkins Medicine
2017
Reutlingen University
2013
Abstract Psoriasis is an inflammatory skin disease with strong neutrophil (PMN) infiltration and high levels of the antimicrobial peptide, LL37. LL37 in complex DNA RNA thought to initiate exacerbation via plasmacytoid dendritic cells. However, source nucleic acids supposed start this initial event remains unknown. We show here that primary murine human PMNs mount a fulminant self-propagating extracellular trap (NET) cytokine response, but independently canonical NET component, DNA....
SARS-CoV-2 is evolving with mutations in the receptor binding domain (RBD) being of particular concern. It important to know how much cross-protection offered between strains following vaccination or infection. Here, we obtain serum and saliva samples from groups vaccinated (Pfizer BNT-162b2), infected uninfected individuals characterize antibody response RBD mutant strains. Vaccinated have a robust humoral after second dose high IgG titers saliva. Antibody responses however show...
The COVID-19 pandemic has resulted in significant morbidity and mortality worldwide. To prevent severe infection, mass vaccination campaigns with several vaccine types are currently underway. We report pathological immunological findings 8 patients who developed vaccine-induced immune thrombotic thrombocytopenia (VITT) after administration of SARS-CoV-2 ChAdOx1 nCoV-19. analyzed patient material using enzyme assays, flow cytometry heparin-induced platelet aggregation assay performed...
The quality and persistence of children's humoral immune response following SARS-CoV-2 infection remains largely unknown but will be crucial to guide pediatric vaccination programs. Here, we examine 548 children 717 adults within 328 households with at least one member a previous laboratory-confirmed infection. We assess serological 3-4 months 11-12 after using bead-based multiplex immunoassay for 23 human coronavirus antigens including its Variants Concern (VOC) endemic coronaviruses...
Abstract Parkinson’s disease-associated kinase LRRK2 has been linked to IFN type II (IFN-γ) response in infections and dopaminergic neuronal loss. However, whether how synergizes with IFN-γ remains unclear. In this study, we employed neurons microglia differentiated from patient-derived induced pluripotent stem cells carrying G2019S, the most common mutation. We show that enhances G2019S-dependent negative regulation of AKT phosphorylation NFAT activation, thereby increasing vulnerability...
Abstract The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis required understand the manifestation progression of COVID-19, monitor seroconversion within general population, support vaccine development. majority currently available commercial serological assays only quantify antibody against individual antigens, limiting our understanding response. To overcome this, we have developed multiplex immunoassay (MultiCoV-Ab) including spike nucleocapsid...
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important track COVID-19 cases and the humoral response infections immunization future vaccines. The aim of this work was develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, quantitative detection human antibodies against serum, plasma, or blood. Recombinant 6xHis-tagged Nucleocapsid protein mobilized on surface Ni2+ beads challenged with serum blood samples obtained from...
BackgroundPatients with chronic renal insufficiency on maintenance haemodialysis face an increased risk of COVID-19 induced mortality and impaired vaccine responses. To date, only a few studies have addressed SARS-CoV-2 elicited immunity in this immunocompromised population.MethodsWe assessed immunogenicity the mRNA BNT162b2 at-risk dialysis patients characterised systemic cellular humoral immune responses serum saliva using interferon γ release assay multiplex-based cytokine immunoglobulin...
Article27 April 2021Open Access Transparent process NeutrobodyPlex—monitoring SARS-CoV-2 neutralizing immune responses using nanobodies Teresa R Wagner orcid.org/0000-0002-1050-5948 Pharmaceutical Biotechnology, Eberhard Karls University, Tuebingen, Germany Natural and Medical Sciences Institute, University of Reutlingen, Search for more papers by this author Elena Ostertag Interfaculty Institute Biochemistry, Philipp D Kaiser Marius Gramlich Natalia Ruetalo Virology Epidemiology Viral...
We report a novel experimental immunotherapeutic approach in patient with metastatic intrahepatic cholangiocarcinoma. In the 5year course of disease, initial tumor mass, two local recurrences and lung metastasis were surgically removed. Lacking alternative treatment options, aiming at induction anti-tumor T cells responses, we initiated personalized multi-peptide vaccination, based on in-depth analysis antigens (immunopeptidome) sequencing.Tumors characterized by immunohistochemistry,...
There is evidence for a relevant role of inflammation in the pathogenesis Parkinson's disease (PD). Mutations LRRK2 gene represent most frequent genetic cause autosomal dominant PD. highly expressed macrophages and microglia suggesting an involvement inflammatory pathways. The objectives are to test (1) whether idiopathic PD LRRK2-associated share common pathways or present distinct profiles (2) non-manifesting mutation carriers with similar aspects as seen PD-affected patients. We assessed...
<h3>Background</h3> We previously showed that the bacterial lipopeptide Pam<sub>3</sub>Cys-Ser-Ser, meanwhile established as a toll-like receptor (TLR) 1/2 ligand, acts strong adjuvant for induction of virus specific CD8<sup>+</sup> T cells in mice, when covalently coupled to synthetic peptide. <h3>Case presentation</h3> now designed new water-soluble Pam<sub>3</sub>Cys-derivative, named XS15 and characterized it vitro by TLR2 NF-κB luciferase reporter assay. Further, capacity activate...
The importance of pre-existing immune responses to seasonal endemic coronaviruses (HCoVs) for the susceptibility SARS-CoV-2 infection and course COVID-19 is subject an ongoing scientific debate. Recent studies postulate that previous HCoV infections can either have a slightly protective or no effect on pathogenesis and, consequently, be neglected risk stratification. Challenging this notion, we provide evidence pre-existing, anti-nucleocapsid antibodies against α-coronaviruses S2...
Abstract SARS-CoV-2 variants accumulating immune escape mutations provide a significant risk to vaccine-induced protection against infection. The novel variant of concern (VoC) Omicron BA.1 and its sub-lineages have the largest number amino acid alterations in Spike protein date. Thus, they may efficiently recognition by neutralizing antibodies, allowing breakthrough infections convalescent vaccinated individuals particular those who only received primary immunization scheme. We analyzed...
Background and purpose The presentation of Parkinson's disease patients with mutations in the LRRK 2 gene ( PD ) is highly variable, suggesting a strong influence modifying factors. In this context, inflammation potential candidate inducing clinical subtypes. Methods An extensive battery peripheral inflammatory markers was measured human serum multicentre cohort 142 from MJFF Consortium, stratified by three different subtypes as recently proposed for idiopathic disease: diffuse/malignant,...
Significance Macrophage plasticity and activation dynamics are under intense investigation because their full complexity cannot be captured by the few predefined markers that commonly used. Macrophages highly adherent respond to surface microstructures in a sensitive manner. Here, we use Raman microspectroscopy imaging study macrophage response defined stimuli transfer these findings macrophages cultured on titanium with varying roughness. We show Raman-based methods can discriminate between...
The SARS-CoV-2 spike (S) glycoprotein is synthesized as a large precursor protein and must be activated by proteolytic cleavage into S1 S2. A recombinant modified vaccinia virus Ankara (MVA) expressing native, full-length S (MVA-SARS-2-S) currently under investigation candidate vaccine in phase I clinical studies. Initial results from immunogenicity monitoring revealed induction of S-specific antibodies binding to S2, but low-level antibody responses the domain. Follow-up investigations...
An involvement of the central-nervous and peripheral, innate adaptive immune system in pathogenesis Parkinson's disease (PD) is nowadays well established.We face several open questions preparation clinical trials aiming at disease-modification by targeting system: Do peripheral (blood) inflammatory profiles reflect central (CSF) processes? Are blood/CSF markers associated with CSF levels neurodegenerative/PD-specific biomarkers?Using a multiplex assay we assessed 41 CSF/serum pairs 453...