A5071566613- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Monoclonal and Polyclonal Antibodies Research
- Long-Term Effects of COVID-19
- Vaccine Coverage and Hesitancy
- Inflammasome and immune disorders
- COVID-19 diagnosis using AI
- COVID-19 Impact on Reproduction
- Bacterial Infections and Vaccines
- Immunotherapy and Immune Responses
- Digestive system and related health
- Biosensors and Analytical Detection
- Child Nutrition and Feeding Issues
- Gut microbiota and health
- Receptor Mechanisms and Signaling
University of Tübingen
2021-2024
University Children's Hospital Tübingen
2021-2023
Universitätsklinikum Tübingen
2021
Klinikum Wilhelmshaven
2021
German Center for Infection Research
2021
SARS-CoV-2 is evolving with mutations in the receptor binding domain (RBD) being of particular concern. It important to know how much cross-protection offered between strains following vaccination or infection. Here, we obtain serum and saliva samples from groups vaccinated (Pfizer BNT-162b2), infected uninfected individuals characterize antibody response RBD mutant strains. Vaccinated have a robust humoral after second dose high IgG titers saliva. Antibody responses however show...
Capsid virus-like particles (cVLP) have proven safe and immunogenic can be a versatile platform to counter pandemics. We aimed clinically test modular cVLP COVID-19 vaccine in individuals who were naive SARS-CoV-2.In this phase 1, single-centre, dose-escalation, adjuvant-selection, open-label clinical trial, we recruited participants at the Radboud University Medical Center Nijmegen, Netherlands, sequentially assigned them seven groups. Eligible healthy, aged 18-55 years, tested negative for...
SARS-CoV-2 antibodies in saliva serve as first line of defense against the virus. They are present mucosa, more precisely saliva, after a recovered infection and also following vaccination. We report here antibody persistence plasma up to 15 months mild COVID-19. The IgG response was measured every two 72 participants using an established validated in-house ELISA assay. In addition, virus inhibitory activity assessed surrogate neutralization test before SARS-CoV-2-specific concentrations...
The rapid emergence of the Omicron variant and its large number mutations led to classification as a concern (VOC) by World Health Organization. Subsequently, evolved into distinct sublineages (eg, BA.1 BA.2), which currently represent majority global infections. Initial studies neutralizing response toward in convalescent vaccinated individuals showed substantial reduction.We assessed antibody (immunoglobulin G [IgG]) binding, ACE2 (angiotensin-converting enzyme 2) binding inhibition, IgG...
Saliva is a body fluid with hitherto unused potential for the assessment of SARS-CoV-2 antibodies. Specific antibodies can indicate past infection and allow to estimate proportion individuals protective immunity. First, we carefully characterized plasma samples obtained from adult control groups without prior using certified reference ELISAs. Simultaneously collected saliva confirmed convalescent negative where then used validate herein newly developed ELISA detection IgG in saliva. The was...
Abstract The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab rayon swab), reagents classical RNA extraction (phenol/guanidine-based protocol commercial kit), intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared gold...
Abstract The rapid emergence of the Omicron variant and its large number mutations has led to classification as a concern (VOC) by WHO(1). Initial studies on neutralizing response towards this within convalescent vaccinated individuals have identified substantial reductions(2-8). However many these sample sets used in were either small, uniform nature, or compared only wild-type (WT) or, at most, few other VOC. Here, we assessed IgG binding, (Angiotensin-Converting Enzyme 2) ACE2 binding...
Abstract The SARS-CoV-2 pandemic virus is consistently evolving with mutations within the receptor binding domain (RBD) 1 being of particular concern 2-4 . To date, there little research into protection offered following vaccination or infection against RBD mutants in emerging variants (UK 3 , South African 5 Mink 6 and Southern California 7 ). investigate this, serum saliva samples were obtained from groups vaccinated (Pfizer BNT-162b2 8 ), infected uninfected individuals. Antibody...
This cross-sectional study evaluates IgG antibody levels in children and adolescents Germany following SARS-CoV-2 infection.
The efficacy of immunization against an airborne pathogen depends in part on its ability to induce antibodies at the major entry site virus, mucosa. Recent studies have revealed that mucosal immunity is poorly activated after vaccination with messenger RNA vaccines, thus failing blocking virus acquisition upon initial exposure. Little information available about induction by inactivated and recombinant coronavirus disease 2019 (COVID-19) vaccines. This study aims investigate this topic.
Background: The efficacy of immunization against an airborne pathogen depends in part on its ability to induce antibodies at the major entry site virus, mucosa. Recent studies have revealed that mucosal immunity is poorly activated after vaccination with mRNA vaccines, thus failing blocking virus acquisition upon initial exposure. Little information available about induction by inactivated and recombinant COVID-19 vaccines. This study aims investigate this topic. Methods: Saliva plasma...
Abstract Short chain fatty acids (SCFAs) are known as metabolites produced from gut microbiome fermenting dietary fibers and resistant starch. They regulators of the interplay between its host may have a role in pathogenesis inflammatory bowel disease (IBD). Free SCFAs influence intestinal epithelial immune cells (e.g. macrophages primary monocytes) through activation free acid receptors FFAR2 FFAR3, inhibition histone deacetylases (HDAC) source energy. to impact cytokine production...
The world has been confronted with the COVID-19 pandemic for more than one year. Severe disease is often found among elderly people, whereas most young children and adolescents show mild symptoms or even remain asymptomatic, so that infection might be undiagnosed. Therefore, only limited epidemiological data on SARS-CoV-2 in adults are available.
<h3>Background</h3> The design of this study was intended to evaluate the use saliva as a reliable non-invasive tool for genomic and immunological surveillance SARS-CoV-2 infection in Republic Congo. <h3>Methods</h3> During cross-sectional study, active determined by detecting RNA using RT-PCR 220 paired oropharyngeal samples (OPS), sequencing genome Oxford nanopore technology. detection anti-SARS-CoV-2 IgG antibody done 148 pair plasma an in-house developed ELISA, reproductivity assay based...
<sec> <title>BACKGROUND</title> The world has been confronted with the COVID-19 pandemic for more than one year. Severe disease is often found among elderly people, whereas most young children and adolescents show mild symptoms or even remain asymptomatic, so that infection might be undiagnosed. Therefore, only limited epidemiological data on SARS-CoV-2 in adults are available. </sec> <title>OBJECTIVE</title> This study aims to determine prevalence of antibodies from city Tübingen, Germany,...