A5040916254- Environmental Sustainability and Education
- Lichen and fungal ecology
- SARS-CoV-2 detection and testing
- Air Quality and Health Impacts
- Microbial Community Ecology and Physiology
- Science and Education Research
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Education Pedagogy and Practices
- Science and Science Education
- Botany and Plant Ecology Studies
- Atmospheric chemistry and aerosols
- Polysaccharides and Plant Cell Walls
- Water Quality and Pollution Assessment
- Maritime Transport Emissions and Efficiency
- Polysaccharides Composition and Applications
- Mycorrhizal Fungi and Plant Interactions
- Municipal Solid Waste Management
- Studies on Chitinases and Chitosanases
- Climate Change Communication and Perception
- Coastal and Marine Management
- Advanced biosensing and bioanalysis techniques
- Vehicle emissions and performance
- Enzyme Production and Characterization
- COVID-19 Clinical Research Studies
Universidade Federal do Paraná
2010-2024
Centro de Estudos e Pesquisas em Educação, Cultura e Ação Comunitária
2022-2023
Universidade Tecnológica Federal do Paraná
2021
Instituto de Desenvolvimento Sustentável Mamirauá
2020
Consejería de Educación de la Junta de Castilla y León
2017
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important track COVID-19 cases and the humoral response infections immunization future vaccines. The aim of this work was develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, quantitative detection human antibodies against serum, plasma, or blood. Recombinant 6xHis-tagged Nucleocapsid protein mobilized on surface Ni2+ beads challenged with serum blood samples obtained from...
The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which characterised by methylation analysis and 1D 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) (1-->6) linkages, structure is more typical basidiomycetes rather than ascomycetes, have linear glucans. other an alpha-glucan with alternating (1-->4) found for first time in Nature. This can be classified as pullulan, has been...
In this study, hypotheses concerning the use of beach ashtrays were experimentally tested. Results indicated that mean rate abandonment equipment was low (1.5%). The amount cigarette stubs (3.4 items/ashtray) greater than amounts other types litter. People with different socioeconomic profiles had perceptions regarding issues associated debris. These results could be useful to prevent contamination these environments and differences in characteristics beachgoers partially explain presence...
A light microscopic and molecular analysis of photobionts in Ramalina Cladonia from coastal habitats Brazil is presented. Bayesian phylogenetic ITS rDNA sequences suggests a Trebouxia lineage which preferentially tropical geographic distribution. This highly diverse clade also includes the morphological similar species higginsiae galapagensis. Within predominantly we distinguish several subclades, three are represented our samples species. Since sexuality has not been recognized coccal...
A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont lichen Ramalina gracilis was carried out in order to compare them with those previously found symbiotic thallus. The isolated thallus fragments, following method Yamamoto, and cultivated a liquid nutrient medium. Freeze-dried cells were defatted, successively water aq. 10% KOH, each at 100 °C. After purification, soluble fractions provided polysaccharide containing (1 ? 5)-linked...
RESUMO A família Annonaceae possui representantes de grande interesse medicinal e o gênero Xylopia é um dos que merecem destaque. Composta por aproximadamente 160 espécies distribuídas na América do Sul, central, África Ásia, as desse podem ser arbustivas ou arbóreas. No Brasil são encontradas nas regiões Norte, Nordeste, Centro-Oeste Centro Sul. Este produz uma variedade metabólitos incluindo alcalóides, amidas, lignóides, acetogeninas terpenóides têm sido investigados como fonte potencial...
Esta pesquisa, qualitativa do tipo documental, analisou o perfil das bolsistas de Produtividade em Pesquisa Conselho Nacional Desenvolvimento Científico e Tecnológico (CNPq), no Brasil, Divulgação Científica. Os dados foram constituídos pelos editais (2013 a 2021) Currículos “Lattes”. resultados apontaram que as mulheres são maioria entre bolsistas; maior parte possui bolsa PQ-2; estão concentradas Sudeste; têm formações diversas. O campo não segue tendência sistema PQ, revelando uma...
Fatty acid components, in both the free and combined form of intact tropical lichen Teloschistes flavicans, its isolated photobiont mycobiont, were analyzed by GC-MS derived methyl esters. Its rDNA analysis confirmed that cultured symbionts belong to genera Trebouxia Teloschistes, respectively. The fatty composition did not correspond those found symbionts, suggesting metabolism is markedly influenced symbiosis. Differences observed during summer (27°C), when main acids saturated winter...
Plant secondary metabolites have attracted considerable attention from the industry as consumers are increasing their interest for natural products over chemically synthesized reagents to be used additives in food, cosmetics or pharmaceuticals. Some plant essential oils show significant antimicrobial properties and this could exploited produce new food preservatives novel formulations. Here we describe extraction, chemical analysis of grass lemon Cymbopogon citratus flexuosus oils. Essential...
Abstract Here we describe a novel immunogenic method to detect COVID-19. The is chromogenic magnetic bead-based ELISA which allows inexpensive and quantitative detection of human IgG or IgM antibodies against SARS-CoV-2 in serum whole blood samples just 12 minutes. As proof concept, compared the performance our new classical ELISA. Person correlation between optical densities obtained using two methods was 0.98, color intensity observed correlated with antibody titers determined by correctly...
Several studies have shown that tree barks can absorb air contaminants, therefore, trees be used as biomonitors to identify the distribution of atmospheric pollutants. The city Paranaguá, located at coast Paraná State in Brazil, hosts largest bulk cargo port Latin America and an elevated number fertilizer processing industries. In this study we coupled X-ray fluorescence spectrometer analysis biomonitor pollutants Paranaguá. We identified a visual correlation between level elements K Cl,...
The cultured photobiont Trebouxia sp. of Ramalina celastri was successively extracted at 100°C with hot water, 2% aqueous KOH, and 10% KOH to give polysaccharide-containing fractions A (2.9%), B (3.9%), C (0.9% yield) respectively. intact biont contained 3.8% amylose, which present in each fraction, identified by a blue color formed iodine solution. In fraction A, following retrogradation from solution, it characterized 13C-NMR spectroscopy. Fraction treated α-amylase water-soluble...